| Literature DB >> 8690760 |
T P Leary1, A S Muerhoff, J N Simons, T J Pilot-Matias, J C Erker, M L Chalmers, G G Schlauder, G J Dawson, S M Desai, I K Mushahwar.
Abstract
Recently, sequences from a putative member of the Flaviviridae, GB virus C (GBV-C), were isolated from the serum of patients with cryptogenic hepatitis. These sequences were 83-99% identical at the nucleotide level. Because of the divergence between these GBV-C isolates, it is likely that the PCR-based detection assay yields false negatives, underestimating dramatically the true prevalence of GBV-C in human hepatitis. We report the design of a GBV-C consensus oligonucleotide primer pair that is superior to those originally described. These primers identify GBV-C sequences in cases of cryptogenic hepatitis, allowing a better estimation of the prevalence of this virus in human populations.Entities:
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Year: 1996 PMID： 8690760 DOI： 10.1016/0166-0934(95)01956-1
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014