Literature DB >> 9350774

Evaluation of the automated COBAS AMPLICOR hepatitis C virus PCR system.

M Poljak1, K Seme, S Koren.   

Abstract

To evaluate the reliability and feasibility of the automated Roche COBAS AMPLICOR PCR system for routine detection of hepatitis C virus (HCV) RNA, a total of 405 serum samples previously tested by an in-house nested PCR and manual Roche AMPLICOR microwell plate HCV test were examined. Complete concordance was found between the results with the HCV COBAS AMPLICOR system and the previously determined HCV RNA status. The automated HCV COBAS AMPLICOR system provides the clinical microbiology laboratory with a specific and sensitive PCR method for rapid and reliable detection of HCV RNA.

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Year:  1997        PMID： 9350774      PMCID： PMC230102          DOI： 10.1128/jcm.35.11.2983-2984.1997

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  12 in total

1.  COBAS AMPLICOR: fully automated RNA and DNA amplification and detection system for routine diagnostic PCR.

Authors:  N DiDomenico; H Link; R Knobel; T Caratsch; W Weschler; Z G Loewy; M Rosenstraus
Journal:  Clin Chem       Date:  1996-12       Impact factor: 8.327

2.  Use of a commercial PCR kit for detection of hepatitis C virus.

Authors:  K Seme; M Poljak
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1995-06       Impact factor: 3.267

3.  Commercial assay for hepatitis C virus RNA.

Authors:  P Tilston; D J Morris; P E Klapper; G Corbitt
Journal:  Lancet       Date:  1994-07-16       Impact factor: 79.321

Review 4.  Overview of international PCR standardization efforts.

Authors:  L E Bockstahler
Journal:  PCR Methods Appl       Date:  1994-04

5.  Determination of hepatitis C virus RNA in the serum by the Amplicor HCV PCR kit.

Authors:  D Prati; C Capelli; P Bosoni; F Mozzi; A Zanella; G Sirchia
Journal:  Vox Sang       Date:  1994       Impact factor: 2.144

6.  Second-generation line probe assay for hepatitis C virus genotyping.

Authors:  L Stuyver; A Wyseur; W van Arnhem; F Hernandez; G Maertens
Journal:  J Clin Microbiol       Date:  1996-09       Impact factor: 5.948

7.  Clinical characterization of a competitive PCR assay for quantitative testing of hepatitis C virus.

Authors:  E P Miskovsky; A V Carrella; K Gutekunst; C A Sun; T C Quinn; D L Thomas
Journal:  J Clin Microbiol       Date:  1996-08       Impact factor: 5.948

Review 8.  The detection, transmission, and outcome of hepatitis C virus infection.

Authors:  M J Alter
Journal:  Infect Agents Dis       Date:  1993-06

9.  Clinical evaluation of a new polymerase chain reaction assay (Amplicor HCV) for detection of hepatitis C virus.

Authors:  S Zeuzem; B Rüster; W K Roth
Journal:  Z Gastroenterol       Date:  1994-06       Impact factor: 2.000

10.  Evaluation of the Amplicor HCV test: experiences after 1 year of routine use in a diagnostic laboratory.

Authors:  K Seme; M Poljak
Journal:  Infection       Date:  1996 Mar-Apr       Impact factor: 3.553
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  10 in total

1.  Hepatitis C virus quantitation: optimization of strategies for detecting low-level viremia.

Authors:  W T Hofgärtner; J A Kant; K E Weck
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

2.  Fully automated detection of hepatitis C virus RNA in serum and whole-blood samples.

Authors:  Harald H Kessler; Alexandra M K Clarici; Evelyn Stelzl; Gerhard Mühlbauer; Elisabeth Daghofer; Brigitte I Santner; Egon Marth; Rudolf E Stauber
Journal:  Clin Diagn Lab Immunol       Date:  2002-11

3.  Novel approach to reduce the hepatitis C virus (HCV) window period: clinical evaluation of a new enzyme-linked immunosorbent assay for HCV core antigen.

Authors:  G Icardi; F Ansaldi; B M Bruzzone; P Durando; S Lee; C de Luigi; P Crovari
Journal:  J Clin Microbiol       Date:  2001-09       Impact factor: 5.948

4.  Therapy for treatment-refractory chronic hepatitis C virus genotype 1b infection: A retrospective analysis.

Authors:  Mehmet Cindoruk; Tarkan Karakan; Selahattin Unal
Journal:  Curr Ther Res Clin Exp       Date:  2005-09

5.  Clinical evaluation of the automated COBAS AMPLICOR HCV MONITOR test version 2.0 for quantifying serum hepatitis C virus RNA and comparison to the quantiplex HCV version 2.0 test.

Authors:  M L Yu; W L Chuang; C Y Dai; S C Chen; Z Y Lin; M Y Hsieh; L Y Wang; W Y Chang
Journal:  J Clin Microbiol       Date:  2000-08       Impact factor: 5.948

6.  Development of a simple and highly sensitive enzyme immunoassay for hepatitis C virus core antigen.

Authors:  K Aoyagi; C Ohue; K Iida; T Kimura; E Tanaka; K Kiyosawa; S Yagi
Journal:  J Clin Microbiol       Date:  1999-06       Impact factor: 5.948

7.  Evaluation of an automated sample preparation protocol for quantitative detection of hepatitis C virus RNA.

Authors:  Evelyn Stelzl; Andrea Kormann-Klement; Josef Haas; Elisabeth Daghofer; Brigitte I Santner; Egon Marth; Harald H Kessler
Journal:  J Clin Microbiol       Date:  2002-04       Impact factor: 5.948

8.  Quantitative assay of hepatitis C virus RNA using an automated extraction system for specific capture with probes and paramagnetic particle separation.

Authors:  Hayato Miyachi; Atsuko Masukawa; Satomi Asai; Toshiaki Miura; Shigeru Tamatsukuri; Toru Hirose; Yasuhiko Ando
Journal:  J Clin Microbiol       Date:  2003-02       Impact factor: 5.948

9.  Evaluation of automated sample preparation and quantitative PCR LCx assay for determination of human immunodeficiency virus type 1 RNA.

Authors:  Zsofia Muller; Evelyn Stelzl; Michael Bozic; Josef Haas; Egon Marth; Harald H Kessler
Journal:  J Clin Microbiol       Date:  2004-04       Impact factor: 5.948

10.  Detection of hepatitis C virus core antigen for early diagnosis of hepatitis C virus infection in plasma donor in China.

Authors:  He-Qiu Zhang; Shao-Bo Li; Guo-Hua Wang; Kun Chen; Xiao-Guo Song; Xiao-Yan Feng
Journal:  World J Gastroenterol       Date:  2007-05-21       Impact factor: 5.742
  10 in total

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