Literature DB >> 9294461

Purification of the Azotobacter vinelandii nifV-encoded homocitrate synthase.

L Zheng1, R H White, D R Dean.   

Abstract

The nifV gene product (NifV) from Azotobacter vinelandii was recombinantly expressed at high levels in Escherichia coli and purified. NifV is a homodimer that catalyzes the condensation of acetyl coenzyme A (acetyl-CoA) and alpha-ketoglutarate. Although alpha-ketoglutarate supports the highest level of activity, NifV will also catalyze the condensation of acetyl-CoA and certain other keto acids. E. coli cells in which a high level of nifV expression is induced excrete homocitrate into the growth medium.

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Year:  1997        PMID： 9294461      PMCID： PMC179493          DOI： 10.1128/jb.179.18.5963-5966.1997

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  19 in total

1.  Enzymatic formation of alpha-isopropylmalic acid, an intermediate in leucine biosynthesis.

Authors:  M STRASSMAN; L N CECI
Journal:  J Biol Chem       Date:  1963-07       Impact factor: 5.157

2.  Homocitrate is a component of the iron-molybdenum cofactor of nitrogenase.

Authors:  T R Hoover; J Imperial; P W Ludden; V K Shah
Journal:  Biochemistry       Date:  1989-04-04       Impact factor: 3.162

3.  The nucleotide sequence of leuA from Salmonella typhimurium.

Authors:  E Ricca; J M Calvo
Journal:  Nucleic Acids Res       Date:  1990-03-11       Impact factor: 16.971

4.  Homocitrate cures the NifV- phenotype in Klebsiella pneumoniae.

Authors:  T R Hoover; J Imperial; P W Ludden; V K Shah
Journal:  J Bacteriol       Date:  1988-04       Impact factor: 3.490

5.  Identification of the V factor needed for synthesis of the iron-molybdenum cofactor of nitrogenase as homocitrate.

Authors:  T R Hoover; A D Robertson; R L Cerny; R N Hayes; J Imperial; V K Shah; P W Ludden
Journal:  Nature       Date:  1987 Oct 29-Nov 4       Impact factor: 49.962

6.  Biochemical and genetic analysis of the nifUSVWZM cluster from Azotobacter vinelandii.

Authors:  M R Jacobson; V L Cash; M C Weiss; N F Laird; W E Newton; D R Dean
Journal:  Mol Gen Genet       Date:  1989-10

7.  nifV-dependent, low-molecular-weight factor required for in vitro synthesis of iron-molybdenum cofactor of nitrogenase.

Authors:  T R Hoover; V K Shah; G P Roberts; P W Ludden
Journal:  J Bacteriol       Date:  1986-09       Impact factor: 3.490

8.  Nitrogenase from nifV mutants of Klebsiella pneumoniae contains an altered form of the iron-molybdenum cofactor.

Authors:  T R Hawkes; P A McLean; B E Smith
Journal:  Biochem J       Date:  1984-01-01       Impact factor: 3.857

9.  A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.

Authors:  S Tabor; C C Richardson
Journal:  Proc Natl Acad Sci U S A       Date:  1985-02       Impact factor: 11.205

10.  Isolation of an iron-molybdenum cofactor from nitrogenase.

Authors:  V K Shah; W J Brill
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205
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  25 in total

Review 1.  Biosynthesis of nitrogenase metalloclusters.

Authors:  Markus W Ribbe; Yilin Hu; Keith O Hodgson; Britt Hedman
Journal:  Chem Rev       Date:  2013-12-13       Impact factor: 60.622

Review 2.  Molybdenum cofactors, enzymes and pathways.

Authors:  Günter Schwarz; Ralf R Mendel; Markus W Ribbe
Journal:  Nature       Date:  2009-08-13       Impact factor: 49.962

3.  (R)-citramalate synthase in methanogenic archaea.

Authors:  D M Howell; H Xu; R H White
Journal:  J Bacteriol       Date:  1999-01       Impact factor: 3.490

Review 4.  Function and evolution of nodulation genes in legumes.

Authors:  Keisuke Yokota; Makoto Hayashi
Journal:  Cell Mol Life Sci       Date:  2011-03-05       Impact factor: 9.261

5.  Transcriptional profiling of nitrogen fixation in Azotobacter vinelandii.

Authors:  Trinity L Hamilton; Marcus Ludwig; Ray Dixon; Eric S Boyd; Patricia C Dos Santos; João C Setubal; Donald A Bryant; Dennis R Dean; John W Peters
Journal:  J Bacteriol       Date:  2011-07-01       Impact factor: 3.490

6.  Algorithmic co-optimization of genetic constructs and growth conditions: application to 6-ACA, a potential nylon-6 precursor.

Authors:  Hui Zhou; Brenda Vonk; Johannes A Roubos; Roel A L Bovenberg; Christopher A Voigt
Journal:  Nucleic Acids Res       Date:  2015-10-30       Impact factor: 16.971

7.  Requirement of NifX and other nif proteins for in vitro biosynthesis of the iron-molybdenum cofactor of nitrogenase.

Authors:  V K Shah; P Rangaraj; R Chatterjee; R M Allen; J T Roll; G P Roberts; P W Ludden
Journal:  J Bacteriol       Date:  1999-05       Impact factor: 3.490

8.  Molybdenum trafficking for nitrogen fixation.

Authors:  Jose A Hernandez; Simon J George; Luis M Rubio
Journal:  Biochemistry       Date:  2009-10-20       Impact factor: 3.162

9.  Host plant genome overcomes the lack of a bacterial gene for symbiotic nitrogen fixation.

Authors:  Tsuneo Hakoyama; Kaori Niimi; Hirokazu Watanabe; Ryohei Tabata; Junichi Matsubara; Shusei Sato; Yasukazu Nakamura; Satoshi Tabata; Li Jichun; Tsuyoshi Matsumoto; Kazuyuki Tatsumi; Mika Nomura; Shigeyuki Tajima; Masumi Ishizaka; Koji Yano; Haruko Imaizumi-Anraku; Masayoshi Kawaguchi; Hiroshi Kouchi; Norio Suganuma
Journal:  Nature       Date:  2009-11-26       Impact factor: 49.962

10.  Metal trafficking for nitrogen fixation: NifQ donates molybdenum to NifEN/NifH for the biosynthesis of the nitrogenase FeMo-cofactor.

Authors:  Jose A Hernandez; Leonardo Curatti; Constantino P Aznar; Zinaida Perova; R David Britt; Luis M Rubio
Journal:  Proc Natl Acad Sci U S A       Date:  2008-08-12       Impact factor: 11.205
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