| Literature DB >> 9864346 |
D M Howell1, H Xu, R H White.
Abstract
The Methanococcus jannaschii gene MJ1392 was cloned, and its protein product was hyperexpressed in Escherichia coli. The resulting protein was purified and shown to catalyze the condensation of pyruvate and acetyl coenzyme A, with the formation of (R)-citramalate. Thus, this gene (cimA) encodes an (R)-citramalate synthase (CimA). This is the first identification of this enzyme, which is likely involved in the biosynthesis of isoleucine.Entities:
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Year: 1999 PMID: 9864346 PMCID: PMC103565
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490