Literature DB >> 2663059

Homocitrate is a component of the iron-molybdenum cofactor of nitrogenase.

T R Hoover1, J Imperial, P W Ludden, V K Shah.   

Abstract

When apodinitrogenase (lacking FeMo-co) was activated with FeMo-co synthesized in vitro in the presence of 3H-labeled homocitrate, label was incorporated into dinitrogenase. The physical association of the label with FeMo-co was demonstrated by reisolation and purification of the cofactor from dinitrogenase. The presence of homocitrate in FeMo-co was established by NMR analysis of the organic acid extracted from dinitrogenase. Quantitation of homocitrate in dinitrogenase showed it to be present at a 1:1 ratio with molybdenum.

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Year:  1989        PMID： 2663059     DOI： 10.1021/bi00433a004

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  34 in total

1.  Identification and characterization of the nifV-nifZ-nifT gene region from the filamentous cyanobacterium Anabaena sp. strain PCC 7120.

Authors:  O Stricker; B Masepohl; W Klipp; H Böhme
Journal:  J Bacteriol       Date:  1997-05       Impact factor: 3.490

2.  Isolation and characterization of the nifUSVW-rpoN gene cluster from Rhodobacter sphaeroides.

Authors:  W G Meijer; F R Tabita
Journal:  J Bacteriol       Date:  1992-06       Impact factor: 3.490

3.  Analysis of Azotobacter vinelandii strains containing defined deletions in the nifD and nifK genes.

Authors:  J G Li; S Tal; A C Robinson; V Dang; B K Burgess
Journal:  J Bacteriol       Date:  1990-10       Impact factor: 3.490

4.  Plausible structure of the iron-molybdenum cofactor of nitrogenase.

Authors:  M S Madden; A M Krezel; R M Allen; P W Ludden; V K Shah
Journal:  Proc Natl Acad Sci U S A       Date:  1992-07-15       Impact factor: 11.205

5.  Identification by suppression subtractive hybridization of Frankia genes induced under nitrogen-fixing conditions.

Authors:  Masatoshi Yamaura; Toshiki Uchiumi; Shiro Higashi; Mikiko Abe; Ken-Ichi Kucho
Journal:  Appl Environ Microbiol       Date:  2010-01-04       Impact factor: 4.792

Review 6.  Function and evolution of nodulation genes in legumes.

Authors:  Keisuke Yokota; Makoto Hayashi
Journal:  Cell Mol Life Sci       Date:  2011-03-05       Impact factor: 9.261

7.  E.p.r.-spectroscopic studies on the molybdenum-iron site of nitrogenase from Clostridium pasteurianum.

Authors:  G N George; R E Bare; H Y Jin; E I Stiefel; R C Prince
Journal:  Biochem J       Date:  1989-08-15       Impact factor: 3.857

8.  Electron-paramagnetic-resonance and magnetic-circular-dichroism studies of the binding of cyanide and thiols to the thiols to the iron-molybdenum cofactor from Klebsiella pneumoniae nitrogenase.

Authors:  A J Richards; D J Lowe; R L Richards; A J Thomson; B E Smith
Journal:  Biochem J       Date:  1994-01-15       Impact factor: 3.857

9.  Nucleotide sequence and genetic analysis of the Rhodobacter capsulatus ORF6-nifUI SVW gene region: possible role of NifW in homocitrate processing.

Authors:  B Masepohl; S Angermüller; S Hennecke; P Hübner; C Moreno-Vivian; W Klipp
Journal:  Mol Gen Genet       Date:  1993-04

10.  Expression of the nifBfdxNnifOQ region of Azotobacter vinelandii and its role in nitrogenase activity.

Authors:  F Rodríguez-Quiñones; R Bosch; J Imperial
Journal:  J Bacteriol       Date:  1993-05       Impact factor: 3.490
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