Literature DB >> 3017921

nifV-dependent, low-molecular-weight factor required for in vitro synthesis of iron-molybdenum cofactor of nitrogenase.

T R Hoover, V K Shah, G P Roberts, P W Ludden.   

Abstract

The molybdate- and ATP-dependent in vitro synthesis of the iron-molybdenum cofactor of nitrogenase requires a low-molecular-weight factor. The factor is present in extracts of nitrogen fixation-derepressed cultures of Klebsiella pneumoniae and Azotobacter vinelandii, but not in extracts of repressed cultures of these bacteria. Analysis of K. pneumoniae Nif mutants has indicated that the nifV gene product is the only nif protein (besides nifA) necessary for the synthesis and accumulation of the factor. The factor is stable to oxygen, temperatures below 120 degrees C, and extremely acidic and basic conditions. The activity of the factor was completely destroyed by dry ashing or digestion with sulfuric acid. The factor has been partially purified by filtration through an Amicon PM-10 DIAFLO membrane and chromatography on DEAE-cellulose, hydroxylapatite, silica gel, and Sephadex G-25.

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Year:  1986        PMID： 3017921      PMCID： PMC215971          DOI： 10.1128/jb.167.3.999-1003.1986

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  22 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Novel metal cluster in the iron-molybdenum cofactor of nitrogenase. Spectroscopic evidence.

Authors:  J Rawlings; V K Shah; J R Chisnell; W J Brill; R Zimmermann; E Münck; W H Orme-Johnson
Journal:  J Biol Chem       Date:  1978-02-25       Impact factor: 5.157

3.  Nitrogenase and nitrogenase reductase associate and dissociate with each catalytic cycle.

Authors:  R V Hageman; R H Burris
Journal:  Proc Natl Acad Sci U S A       Date:  1978-06       Impact factor: 11.205

4.  Regulation and characterization of protein products coded by the nif (nitrogen fixation) genes of Klebsiella pneumoniae.

Authors:  G P Roberts; T MacNeil; D MacNeil; W J Brill
Journal:  J Bacteriol       Date:  1978-10       Impact factor: 3.490

5.  The nitrogenase system from Azotobacter: two-enzyme requirement for N2 reduction, ATP-dependent H2 evolution, and ATP hydrolysis.

Authors:  W A Bulen; J R LeComte
Journal:  Proc Natl Acad Sci U S A       Date:  1966-09       Impact factor: 11.205

6.  Gene-product relationships of the nif regulon of Klebsiella pneumoniae.

Authors:  G P Roberts; W J Brill
Journal:  J Bacteriol       Date:  1980-10       Impact factor: 3.490

7.  Electron transport to nitrogenase in Klebsiella pneumoniae.

Authors:  D Nieva-Gómez; G P Roberts; S Klevickis; W J Brill
Journal:  Proc Natl Acad Sci U S A       Date:  1980-05       Impact factor: 11.205

8.  Acetylene reduction by the iron-molybdenum cofactor from nitrogenase.

Authors:  V K Shah; J R Chisnell; W J Brill
Journal:  Biochem Biophys Res Commun       Date:  1978-03-15       Impact factor: 3.575

9.  Fine-structure mapping and complementation analysis of nif (nitrogen fixation) genes in Klebsiella pneumoniae.

Authors:  T MacNeil; D MacNeil; G P Roberts; M A Supiano; W J Brill
Journal:  J Bacteriol       Date:  1978-10       Impact factor: 3.490

10.  Isolation of an iron-molybdenum cofactor from nitrogenase.

Authors:  V K Shah; W J Brill
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205
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  12 in total

1.  Isolation and characterization of the nifUSVW-rpoN gene cluster from Rhodobacter sphaeroides.

Authors:  W G Meijer; F R Tabita
Journal:  J Bacteriol       Date:  1992-06       Impact factor: 3.490

2.  Purification and characterization of the nifN and nifE gene products from Azotobacter vinelandii mutant UW45.

Authors:  T D Paustian; V K Shah; G P Roberts
Journal:  Proc Natl Acad Sci U S A       Date:  1989-08       Impact factor: 11.205

3.  ApoNifH functions in iron-molybdenum cofactor synthesis and apodinitrogenase maturation.

Authors:  P Rangaraj; V K Shah; P W Ludden
Journal:  Proc Natl Acad Sci U S A       Date:  1997-10-14       Impact factor: 11.205

4.  Purification of the Azotobacter vinelandii nifV-encoded homocitrate synthase.

Authors:  L Zheng; R H White; D R Dean
Journal:  J Bacteriol       Date:  1997-09       Impact factor: 3.490

5.  Homocitrate cures the NifV- phenotype in Klebsiella pneumoniae.

Authors:  T R Hoover; J Imperial; P W Ludden; V K Shah
Journal:  J Bacteriol       Date:  1988-04       Impact factor: 3.490

6.  Synthesis of the iron-molybdenum cofactor of nitrogenase is inhibited by a low-molecular-weight metabolite of Klebsiella pneumoniae.

Authors:  D M Downs; P W Ludden; V K Shah
Journal:  J Bacteriol       Date:  1990-10       Impact factor: 3.490

7.  Biochemical and genetic analysis of the nifUSVWZM cluster from Azotobacter vinelandii.

Authors:  M R Jacobson; V L Cash; M C Weiss; N F Laird; W E Newton; D R Dean
Journal:  Mol Gen Genet       Date:  1989-10

8.  Iron-molybdenum cofactor synthesis in Azotobacter vinelandii Nif- mutants.

Authors:  J Imperial; V K Shah; R A Ugalde; P W Ludden; W J Brill
Journal:  J Bacteriol       Date:  1987-04       Impact factor: 3.490

9.  Effects of homocitrate, homocitrate lactone, and fluorohomocitrate on nitrogenase in NifV- mutants of Azotobacter vinelandii.

Authors:  M S Madden; T D Paustian; P W Ludden; V K Shah
Journal:  J Bacteriol       Date:  1991-09       Impact factor: 3.490

Review 10.  Biosynthesis of Nitrogenase Cofactors.

Authors:  Stefan Burén; Emilio Jiménez-Vicente; Carlos Echavarri-Erasun; Luis M Rubio
Journal:  Chem Rev       Date:  2020-01-24       Impact factor: 60.622
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