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Literature DB >> 928069

Highly efficient translation of messenger RNA in cell-free extracts prepared from L-cells.

Abstract

Micrococcal nuclease was used to eliminate endogenous protein synthesis in extracts prepared from L cells. The nuclease can be inhibited subsequently with 2'-deoxythymidine-3', 5'-diphosphate. Nuclease-treated extracts primed with exogenous reovirus mRNA, synthesized full length polypeptides with linear kinetics for almost two hours leading to stimulation of the order of 10(4) times over endogenous background. On the average, between 40 and 50 molecules of polypeptide were synthesized per molecule of mRNA.

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Year: 1977 PMID： 928069 PMCID： PMC342674 DOI： 10.1093/nar/4.10.3581

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

15 in total

1. Studies on the mechanism of action of micrococcal nuclease. 1. Degradation of thymus deoxyribonucleic acid.

Authors: W K ROBERTS; C A DEKKER; G W RUSHIZKY; C A KNIGHT
Journal: Biochim Biophys Acta Date: 1962-05-14

2. Regulated transcription of the genomes of defective virions and temperature-sensitive mutants of reovirus.

Authors: D A Spandidos; G Krystal; A F Graham
Journal: J Virol Date: 1976-04 Impact factor: 5.103

3. Translation of reovirus mRNA, poliovirus RNA and bacteriophage Qbeta RNA in cell-free extracts of mammalian cells.

Authors: L Villa-Komaroff; M McDowell; D Baltimore; H F Lodish
Journal: Methods Enzymol Date: 1974 Impact factor: 1.600

4. Polypeptide components of virions, top component and cores of reovirus type 3.

Authors: R E Smith; H J Zweerink; W K Joklik
Journal: Virology Date: 1969-12 Impact factor: 3.616

5. Catalytic properties and specificity of the extracellular nuclease of Staphylococcus aureus.

Authors: P Cuatrecasas; S Fuchs; C B Anfinsen
Journal: J Biol Chem Date: 1967-04-10 Impact factor: 5.157

6. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.

Authors: B E Roberts; B M Paterson
Journal: Proc Natl Acad Sci U S A Date: 1973-08 Impact factor: 11.205

1. Cell-free translation systems prepared from starfish oocytes faithfully reflect in vivo activity; mRNA and initiation factors stimulate supernatants from immature oocytes.

Authors: Z Xu; M B Hille
Journal: Cell Regul Date: 1990-12

2. Reovirus guanylyltransferase is L2 gene product lambda 2.

Authors: D R Cleveland; H Zarbl; S Millward
Journal: J Virol Date: 1986-10 Impact factor: 5.103

3. The viral protein sigma 3 participates in translation of late viral mRNA in reovirus-infected L cells.

Authors: R Lemieux; G Lemay; S Millward
Journal: J Virol Date: 1987-08 Impact factor: 5.103

4. Identification and characterization of a third complementation group of emetine-resistant Chinese hamster cell mutants.

Authors: J J Wasmuth; J M Hill; L S Vock
Journal: Mol Cell Biol Date: 1981-01 Impact factor: 4.272

5. mRNA capping enzymes are masked in reovirus progeny subviral particles.

Authors: D Skup; S Millward
Journal: J Virol Date: 1980-05 Impact factor: 5.103

6. Shutoff of neuroblastoma cell protein synthesis by Semliki Forest virus: loss of ability of crude initiation factors to recognize early Semliki Forest virus and host mRNA's.

Authors: H van Steeg; A Thomas; S Verbeek; M Kasperaitis; H O Voorma; R Benne
Journal: J Virol Date: 1981-05 Impact factor: 5.103

Highly efficient translation of messenger RNA in cell-free extracts prepared from L-cells.

1. Studies on the mechanism of action of micrococcal nuclease. 1. Degradation of thymus deoxyribonucleic acid.

2. Regulated transcription of the genomes of defective virions and temperature-sensitive mutants of reovirus.

3. Translation of reovirus mRNA, poliovirus RNA and bacteriophage Qbeta RNA in cell-free extracts of mammalian cells.

4. Polypeptide components of virions, top component and cores of reovirus type 3.

5. Catalytic properties and specificity of the extracellular nuclease of Staphylococcus aureus.

6. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.

7. An efficient mRNA-dependent translation system from reticulocyte lysates.

Review 8. Translational control of protein synthesis.

9. The rye embryo system as an alternative to the wheat system for protein synthesis in vitro.

10. Increased efficiency of exogenous messenger RNA translation in a Krebs ascites cell lysate.

1. Cell-free translation systems prepared from starfish oocytes faithfully reflect in vivo activity; mRNA and initiation factors stimulate supernatants from immature oocytes.

2. Reovirus guanylyltransferase is L2 gene product lambda 2.

3. The viral protein sigma 3 participates in translation of late viral mRNA in reovirus-infected L cells.

4. Identification and characterization of a third complementation group of emetine-resistant Chinese hamster cell mutants.

5. mRNA capping enzymes are masked in reovirus progeny subviral particles.

6. Shutoff of neuroblastoma cell protein synthesis by Semliki Forest virus: loss of ability of crude initiation factors to recognize early Semliki Forest virus and host mRNA's.

7. mRNA discrimination in extracts from uninfected and reovirus-infected L-cells.

8. Reovirus progeny subviral particles synthesize uncapped mRNA.

9. Reovirus-induced modification of cap-dependent translation in infected L cells.