Literature DB >> 4338590

Increased efficiency of exogenous messenger RNA translation in a Krebs ascites cell lysate.

S Metafora, M Terada, L W Dow, P A Marks, A Bank.   

Abstract

Addition of a 0.5 M KCl wash fraction from rabbit reticulocyte ribosomes causes a 3- to 10-fold increase in the extent of translation of natural mRNAs by Krebs-cell lysates. In the presence of the wash fraction, 1 pmol of rabbit or mouse 10S RNA directs the incorporation of 80 pmol of leucine into rabbit globin. The addition of human 10S RNA results in the synthesis of equal amounts of human alpha and beta chains, identified by column chromatography. The stimulation by the wash fraction is almost completely dependent on added mammalian tRNA. In contrast to the wash fraction from rabbit reticulocytes, the wash fraction isolated from Krebs-cell ribosomes is inhibitory to both endogenous and exogenous mRNA translation. The stimulation by the wash fraction from rabbit ribosomes is not specific for globin mRNAs, but also increases endogenous, phage Qbeta, and viral RNA-directed protein synthesis.

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Year:  1972        PMID： 4338590      PMCID： PMC426686          DOI： 10.1073/pnas.69.5.1299

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  20 in total

1.  Stimulation of globin synthesis by RNA purified from reticulocyte supernatant fraction.

Authors:  L Grossbard; J Banks; P A Marks
Journal:  Arch Biochem Biophys       Date:  1968-05       Impact factor: 4.013

2.  Role of methionine in the initiation of haemoglobin synthesis.

Authors:  R Jackson; T Hunter
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

3.  Isolation and partial characterization of reticulocyte factors M1 and M2.

Authors:  D A Shafritz; W F Anderson
Journal:  J Biol Chem       Date:  1970-11-10       Impact factor: 5.157

4.  Mammalian cell-free protein synthesis directed by viral ribonucleic acid.

Authors:  M Mathews; A Korner
Journal:  Eur J Biochem       Date:  1970-12

5.  The synthesis of mouse hemoglobin beta-chains in a rabbit reticulocyte cell-free system programmed with mouse reticulocyte 9S RNA.

Authors:  R E Lockard; J B Lingrel
Journal:  Biochem Biophys Res Commun       Date:  1969-10-08       Impact factor: 3.575

6.  Protein chain initiation in rabbit reticulocytes.

Authors:  D B Wilson; H M Dintzis
Journal:  Proc Natl Acad Sci U S A       Date:  1970-08       Impact factor: 11.205

7.  Abnormal human haemoglobins. Separation and characterization of the alpha and beta chains by chromatography, and the determination of two new variants, hb Chesapeak and hb J (Bangkok).

Authors:  J B Clegg; M A Naughton; D J Weatherball
Journal:  J Mol Biol       Date:  1966-08       Impact factor: 5.469

8.  Factors controlling amino acid incorporation by ribosomes from krebs II mouse ascites-tumour cells.

Authors:  I M Kerr; N Cohen; T S Work
Journal:  Biochem J       Date:  1966-03       Impact factor: 3.857

9.  Cell-free hemoglobin synthesis in beta-thalassemia.

Authors:  J M Gilbert; A G Thornton; A W Nienhuis; W F Anderson
Journal:  Proc Natl Acad Sci U S A       Date:  1970-12       Impact factor: 11.205

10.  The mechanism of protein synthesis activation after fertilization of sea urchin eggs.

Authors:  S Metafora; L Felicetti; R Gambino
Journal:  Proc Natl Acad Sci U S A       Date:  1971-03       Impact factor: 11.205
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  26 in total

1.  Interferon activity produced by translation of human interferon messenger RNA in cell-free ribosomal systems and in Xenopus oöcytes.

Authors:  F H Reynolds; E Premkumar; P M Pitha
Journal:  Proc Natl Acad Sci U S A       Date:  1975-12       Impact factor: 11.205

2.  Regulation of protein synthesis at the translational level in neuroblastoma cells.

Authors:  F Zucco; M Persico; A Felsani; S Metafora; G Augusti-Tocco
Journal:  Proc Natl Acad Sci U S A       Date:  1975-06       Impact factor: 11.205

3.  Isolation and characterization of collagen messenger RNA*.

Authors:  L Wang; C L Simões; S Sonohara; M Brentani; H F Andrade; S M da Silva; J M Salles; N Marques; R Brentani
Journal:  Nucleic Acids Res       Date:  1975-05       Impact factor: 16.971

4.  Highly efficient translation of messenger RNA in cell-free extracts prepared from L-cells.

Authors:  D Skup; S Millward
Journal:  Nucleic Acids Res       Date:  1977-10       Impact factor: 16.971

5.  In vitro translation of oogenetic messenger RNA of sea urchin eggs and picornavirus RNA with a cell-free system from sarcoma 180.

Authors:  N Jenkins; M W Taylor; R A Raff
Journal:  Proc Natl Acad Sci U S A       Date:  1973-12       Impact factor: 11.205

6.  Biological activity of 125iodine labelled globin mRNA in an Ehrlich ascites cell-free system.

Authors:  W Knöchel
Journal:  Mol Biol Rep       Date:  1974-03       Impact factor: 2.316

7.  Globin messenger RNA activity in erythroid precursor cells and the effect of erythropoietin.

Authors:  M Terada; L Cantor; S Metafora; R A Rifkind; A Bank; P A Marks
Journal:  Proc Natl Acad Sci U S A       Date:  1972-12       Impact factor: 11.205

8.  Specific changes in the messenger ribonucleic acid content of the rat ventral prostate gland after androgenic stimulation. Evidence from the synthesis of aldolase messenger ribonucleic acid.

Authors:  W I Mainwaring; F R Mangan; R A Irving; D A Jones
Journal:  Biochem J       Date:  1974-11       Impact factor: 3.857

Review 9.  The molecular basis of disorders of human hemoglobin synthesis.

Authors:  F Ramirez; J G Mears; A Bank
Journal:  Mol Cell Biochem       Date:  1980-08-16       Impact factor: 3.396

10.  Nucleotide sequences of human globin messenger RNA.

Authors:  C A Marotta; B G Forget; S M Weissman; I M Verma; R P McCaffrey; D Baltimore
Journal:  Proc Natl Acad Sci U S A       Date:  1974-06       Impact factor: 11.205
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