Literature DB >> 4525168

Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.

B E Roberts, B M Paterson.   

Abstract

Extracts prepared from commercially available wheat germ efficiently translate messenger RNAs from either viral or eukaryotic origin. The addition of tobacco mosaic virus RNA stimulated amino-acid incorporation more than 100-times and rabbit globin 9S RNA between 20- and 30-times. The in vitro product directed by globin 9S RNA comigrated precisely with authentic rabbit globin on sodium dodecyl sulfate-polyacrylamide gels. Furthermore, during high voltage ionophoresis two [(35)S]methionine-labeled tryptic peptides synthesized in vitro comigrated in two dimensions with alphaT5 and betaT5 tryptic peptides from authentic [(35)S]methionine-labeled rabbit globin.

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Year:  1973        PMID： 4525168      PMCID： PMC433729          DOI： 10.1073/pnas.70.8.2330

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  28 in total

1.  Characterization of the polypeptides formed in response to encephalomyocarditis virus ribonucleic acid in a cell-free system from mouse ascites tumor cells.

Authors:  I M Kerr; R E Brown; D R Tovell
Journal:  J Virol       Date:  1972-07       Impact factor: 5.103

2.  Translation of reovirus messenger RNAs synthetesized in vitro into reovirus polypeptides by several mammalian cell-free extracts.

Authors:  M J McDowell; W K Joklik; L Villa-Komaroff; H F Lodish
Journal:  Proc Natl Acad Sci U S A       Date:  1972-09       Impact factor: 11.205

3.  Rabbit haemoglobin synthesis in frog cells: the translation of reticulocyte 9 s RNA in frog oocytes.

Authors:  C D Lane; G Marbaix; J B Gurdon
Journal:  J Mol Biol       Date:  1971-10-14       Impact factor: 5.469

4.  Hemoglobin synthesis during rabbit reticulocyte maturation in vitro.

Authors:  H M Schulman
Journal:  Biochim Biophys Acta       Date:  1968-01-29

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Synthesis of and chains of rabbit hemoglobin in a cell-free extract from Krebs II ascites cells.

Authors:  D Housman; R Pemberton; R Taber
Journal:  Proc Natl Acad Sci U S A       Date:  1971-11       Impact factor: 11.205

7.  The synthesis of mouse hemoglobin beta-chains in a rabbit reticulocyte cell-free system programmed with mouse reticulocyte 9S RNA.

Authors:  R E Lockard; J B Lingrel
Journal:  Biochem Biophys Res Commun       Date:  1969-10-08       Impact factor: 3.575

8.  Synthesis of myosin on heterologous ribosomes.

Authors:  S M Heywood
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1969

9.  Purification and properties of biologically active messenger RNA for a myeloma light chain.

Authors:  D Swan; H Aviv; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1972-07       Impact factor: 11.205

10.  Translation of exogenous messenger RNA for hemoglobin on reticulocyte and liver ribosomes.

Authors:  P M Prichard; D J Picciano; D G Laycock; W F Anderson
Journal:  Proc Natl Acad Sci U S A       Date:  1971-11       Impact factor: 11.205
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  386 in total

1.  A highly efficient and robust cell-free protein synthesis system prepared from wheat embryos: plants apparently contain a suicide system directed at ribosomes.

Authors:  K Madin; T Sawasaki; T Ogasawara; Y Endo
Journal:  Proc Natl Acad Sci U S A       Date:  2000-01-18       Impact factor: 11.205

2.  Synthesis of the early light-inducible protein is controlled by blue light and related to light stress.

Authors:  I Adamska; I Ohad; K Kloppstech
Journal:  Proc Natl Acad Sci U S A       Date:  1992-04-01       Impact factor: 11.205

3.  Purification of influenza viral complementary RNA: its genetic content and activity in wheat germ cell-free extracts.

Authors:  P R Etkind; R M Krug
Journal:  J Virol       Date:  1975-12       Impact factor: 5.103

4.  Synthesis of delta crystallin from embryonic chick lens messenger ribonucleoprotein complex.

Authors:  G C Lavers
Journal:  Mol Biol Rep       Date:  1976-09       Impact factor: 2.316

5.  A cell-free protein synthesis system for high-throughput proteomics.

Authors:  Tatsuya Sawasaki; Tomio Ogasawara; Ryo Morishita; Yaeta Endo
Journal:  Proc Natl Acad Sci U S A       Date:  2002-10-30       Impact factor: 11.205

6.  Differential effects of methyl jasmonate on the expression of the early light-inducible proteins and other light-regulated genes in barley.

Authors:  I Wierstra; K Kloppstech
Journal:  Plant Physiol       Date:  2000-10       Impact factor: 8.340

7.  Elastase regulates the synthesis of its inhibitor, alpha 1-proteinase inhibitor, and exaggerates the defect in homozygous PiZZ alpha 1 PI deficiency.

Authors:  D H Perlmutter; J Travis; P I Punsal
Journal:  J Clin Invest       Date:  1988-06       Impact factor: 14.808

8.  Synthesis of stress proteins is increased in individuals with homozygous PiZZ alpha 1-antitrypsin deficiency and liver disease.

Authors:  D H Perlmutter; M J Schlesinger; J A Pierce; P I Punsal; A L Schwartz
Journal:  J Clin Invest       Date:  1989-11       Impact factor: 14.808

9.  Relationship between structure of the 5' noncoding region of viral mRNA and efficiency in the initiation step of protein synthesis in a eukaryotic system.

Authors:  K Yamaguchi; S Hidaka; K Miura
Journal:  Proc Natl Acad Sci U S A       Date:  1982-02       Impact factor: 11.205

10.  Metabolism As a Function of Water Potential in Air-Dry Seeds of Charlock (Sinapis arvensis L.).

Authors:  M Edwards
Journal:  Plant Physiol       Date:  1976-08       Impact factor: 8.340
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