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Literature DB >> 9196221

Comparison of Madin-Darby canine kidney cells (MDCK) with a green monkey continuous cell line (Vero) and human lung embryonated cells (MRC-5) in the isolation of influenza A virus from nasopharyngeal aspirates by shell vial culture.

J Reina¹, V Fernandez-Baca, I Blanco, M Munar.

Abstract

We report a comparative study of the MDCK, Vero, and MRC-5 cell lines in the isolation of the influenza A (IA) virus. We studied 746 samples in which 63 IA viruses were isolated. The MDCK line displayed 100% sensitivity, the Vero line displayed 71.4%, and the MRC-5 displayed 57.1%. The MDCK line showed a statistically significant difference with respect to the Vero line (P = 0.001) and the MRC-5 line (P = 0.001). The quantitative sensitivity analysis showed the MDCK line to be superior to the other lines. It seems that the MDCK line is still one of the most recommendable for the isolation of the IA virus from respiratory samples.

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Year: 1997 PMID： 9196221 PMCID： PMC229869 DOI： 10.1128/jcm.35.7.1900-1901.1997

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

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7. Rapid diagnosis of influenza A infection by direct immunofluorescence of nasopharyngeal aspirates in adults.

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8. Isolation of influenza virus in human lung embryonated fibroblast cells (MRC-5) from clinical samples.

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10. Replication of influenza A viruses in a green monkey kidney continuous cell line (Vero).

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2. QuickVue influenza test for rapid detection of influenza A and B viruses in a pediatric population.

Authors: Caroline Quach; Diane Newby; Ghislaine Daoust; Earl Rubin; Jane McDonald
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3. Evaluation of a new dot blot enzyme immunoassay (directigen flu A+B) for simultaneous and differential detection of influenza a and B virus antigens from respiratory samples.

Authors: Jordi Reina; Emma Padilla; Fermin Alonso; Enrique Ruiz De Gopegui; Maria Munar; Margarita Mari
Journal: J Clin Microbiol Date: 2002-09 Impact factor: 5.948

Comparison of Madin-Darby canine kidney cells (MDCK) with a green monkey continuous cell line (Vero) and human lung embryonated cells (MRC-5) in the isolation of influenza A virus from nasopharyngeal aspirates by shell vial culture.

1. Enhancing effect of centrifugation on isolation of influenza virus from clinical specimens.

2. Evaluation of a direct immunofluorescence assay, dot-blot enzyme immunoassay, and shell vial culture in the diagnosis of lower respiratory tract infections caused by influenza A virus.

3. Canine kidney cell line for isolation of respiratory viruses.

4. Successful use of shell vial centrifugation and 16 to 18-hour immunofluorescent staining for the detection of influenza A and B in clinical specimens.

5. Detection of influenza virus by centrifugal inoculation of MDCK cells and staining with monoclonal antibodies.

6. Comparison of different tissue cultures for isolation and quantitation of influenza and parainfluenza viruses.

7. Rapid diagnosis of influenza A infection by direct immunofluorescence of nasopharyngeal aspirates in adults.

8. Isolation of influenza virus in human lung embryonated fibroblast cells (MRC-5) from clinical samples.

9. Rapid viral diagnosis of acute respiratory infections: comparison of enzyme-linked immunosorbent assay and the immunofluorescence technique for detection of viral antigens in nasopharyngeal secretions.

10. Replication of influenza A viruses in a green monkey kidney continuous cell line (Vero).

1. Comparison of rapid centrifugation assay with conventional tissue culture method for isolation of dengue 2 virus in C6/36-HT cells.

2. QuickVue influenza test for rapid detection of influenza A and B viruses in a pediatric population.

3. Evaluation of a new dot blot enzyme immunoassay (directigen flu A+B) for simultaneous and differential detection of influenza a and B virus antigens from respiratory samples.

4. Establishment and characterization of a Madin-Darby canine kidney reporter cell line for influenza A virus assays.

Review 5. Role of cell culture for virus detection in the age of technology.

Review 6. Cell culture-based influenza vaccines: A necessary and indispensable investment for the future.

7. Cell culture-derived flu vaccine: Present and future.

8. Comparison of embryonated chicken eggs with MDCK cell culture for the isolation of swine influenza virus.

9. Characterization of a porcine lung epithelial cell line suitable for influenza virus studies.

10. High permissivity of human HepG2 hepatoma cells for influenza viruses.