Literature DB >> 10970418

Comparison of rapid centrifugation assay with conventional tissue culture method for isolation of dengue 2 virus in C6/36-HT cells.

R Rodríguez Roche1, M Alvarez, M G Guzmán, L Morier, G Kourí.   

Abstract

A rapid centrifugation assay was compared with conventional tube cell culture for dengue virus isolation in both sera and autopsy samples from dengue and dengue hemorrhagic fever/dengue shock syndrome fatal cases. The rapid centrifugation assay allowed isolation of virus from 16.6% more samples than the conventional method, and it shortened the time for dengue virus detection. Finally, it allowed the isolation of dengue 2 virus in 42.8% of tissue samples from five fatal cases. Our results suggest that the rapid centrifugation assay may be useful for detection of dengue virus in clinical specimens.

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Year:  2000        PMID： 10970418      PMCID： PMC87421     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  23 in total

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Journal:  Am J Trop Med Hyg       Date:  1952-01       Impact factor: 2.345

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Authors:  C A Gleaves; D J Wilson; A D Wold; T F Smith
Journal:  J Clin Microbiol       Date:  1985-01       Impact factor: 5.948

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Journal:  Am J Trop Med Hyg       Date:  1974-11       Impact factor: 2.345

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Journal:  Am J Trop Med Hyg       Date:  1968-05       Impact factor: 2.345

5.  Rapid detection of influenza virus by shell vial assay with monoclonal antibodies.

Authors:  M J Espy; T F Smith; M W Harmon; A P Kendal
Journal:  J Clin Microbiol       Date:  1986-10       Impact factor: 5.948

6.  Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen.

Authors:  C A Gleaves; T F Smith; E A Shuster; G R Pearson
Journal:  J Clin Microbiol       Date:  1984-06       Impact factor: 5.948

7.  Isolation of dengue viruses by intracerebral inoculation of mosquito larvae.

Authors:  S K Lam; C B Chew; G K Poon; S Ramalingam; S C Seow; T Pang
Journal:  J Virol Methods       Date:  1986-09       Impact factor: 2.014

8.  [Isolation of dengue virus type 2 from the serum of patients using the suckling mouse and cultured LLCMK2 cells].

Authors:  M G Guzmán Tirado; G Kourí Flores; M Soler Nodarse; L Morier Díaz; S Vázquez Ramudo
Journal:  Rev Cubana Med Trop       Date:  1984 Jan-Apr

9.  A method for the isolation and identification of dengue viruses, using mosquito cell cultures.

Authors:  R B Tesh
Journal:  Am J Trop Med Hyg       Date:  1979-11       Impact factor: 2.345

10.  Simplified plaque reduction neutralization assay for dengue viruses by semimicro methods in BHK-21 cells: comparison of the BHK suspension test with standard plaque reduction neutralization.

Authors:  D M Morens; S B Halstead; P M Repik; R Putvatana; N Raybourne
Journal:  J Clin Microbiol       Date:  1985-08       Impact factor: 5.948
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  16 in total

1.  Molecular epidemiology of yellow fever in Bolivia from 1999 to 2008.

Authors:  Cécile Baronti; Norma Janeth Velasquez Goitia; Shelley Cook; Yelin Roca; Jimmy Revollo; Jorge Vargas Flores; Xavier de Lamballerie
Journal:  Vector Borne Zoonotic Dis       Date:  2010-10-06       Impact factor: 2.133

Review 2.  Role of cell culture for virus detection in the age of technology.

Authors:  Diane S Leland; Christine C Ginocchio
Journal:  Clin Microbiol Rev       Date:  2007-01       Impact factor: 26.132

3.  Dengue: where are we today?

Authors:  Maria Guadalupe Guzman; Susana Vázquez; Gustavo Kouri
Journal:  Malays J Med Sci       Date:  2009-07

4.  Primary and secondary infections of Macaca fascicularis monkeys with Asian and American genotypes of dengue virus 2.

Authors:  Lidice Bernardo; Alienys Izquierdo; Irina Prado; Delfina Rosario; Mayling Alvarez; Emidalys Santana; Jorge Castro; Rafael Martínez; Rosmari Rodríguez; Luis Morier; Gerardo Guillén; María G Guzmán
Journal:  Clin Vaccine Immunol       Date:  2007-12-19

5.  Characterization of the early events in dengue virus cell entry by biochemical assays and single-virus tracking.

Authors:  Hilde M van der Schaar; Michael J Rust; Barry-Lee Waarts; Heidi van der Ende-Metselaar; Richard J Kuhn; Jan Wilschut; Xiaowei Zhuang; Jolanda M Smit
Journal:  J Virol       Date:  2007-08-29       Impact factor: 5.103

6.  Monoclonal antibody to dengue capsid protein: its application in dengue studies.

Authors:  Y Vazquez; Maritza Pupo-Antúnez; S V Vazquez; V Capó; G Torres; Y Caballero; A Sánchez; D Limonta; M Alvarez; M G Guzmán
Journal:  MAbs       Date:  2009-03-21       Impact factor: 5.857

7.  Molecular epidemiological analysis of dengue fever in Bolivia from 1998 to 2008.

Authors:  Yelin Roca; Cécile Baronti; Roberto Jimmy Revollo; Shelley Cook; Roxana Loayza; Laetitia Ninove; Roberto Torrez Fernandez; Jorge Vargas Flores; Jean-Pierre Herve; Xavier de Lamballerie
Journal:  Vector Borne Zoonotic Dis       Date:  2009-06       Impact factor: 2.133

8.  Dengue virus identification by transmission electron microscopy and molecular methods in fatal dengue hemorrhagic fever.

Authors:  D Limonta; V Falcón; G Torres; V Capó; I Menéndez; D Rosario; Y Castellanos; M Alvarez; R Rodríguez-Roche; M C de la Rosa; A Pavón; L López; K González; G Guillén; J Diaz; M G Guzmán
Journal:  Infection       Date:  2012-04-20       Impact factor: 7.455

9.  Dengue virus type 3, Cuba, 2000-2002.

Authors:  Rosmari Rodriguez-Roche; Mayling Alvarez; Edward C Holmes; Lidice Bernardo; Gustavo Kouri; Ernest A Gould; Scott Halstead; Maria G Guzman
Journal:  Emerg Infect Dis       Date:  2005-05       Impact factor: 6.883

10.  Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca).

Authors:  Feng-Shan Gao; Gui-Xue Hu; Xian-zhu Xia; Yu-Wei Gao; Ya-Duo Bai; Xiao-Huan Zou
Journal:  J Vet Sci       Date:  2009-09       Impact factor: 1.672
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