Literature DB >> 2380390

Enhancing effect of centrifugation on isolation of influenza virus from clinical specimens.

M Seno1, Y Kanamoto, S Takao, N Takei, S Fukuda, H Umisa.   

Abstract

The use of centrifugation (700 x g, 60 min) in a plaque assay markedly increased (mean, 2.9-fold) the infectivity of all 42 influenza virus strains tested, compared with no centrifugation. Of 13 influenza virus strains isolated from 390 clinical specimens, 9 (69%) were efficiently isolated by the centrifugation assay compared with conventional culture methods. The centrifugation assay may be useful for isolating the influenza virus from clinical specimens.

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Year:  1990        PMID： 2380390      PMCID： PMC268014          DOI： 10.1128/jcm.28.7.1669-1670.1990

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  14 in total

1.  Ultracentrifugal inoculation of herpes simplex virus.

Authors:  R B Tenser
Journal:  Infect Immun       Date:  1978-07       Impact factor: 3.441

2.  Rapid diagnosis of influenza A and B by 24-h fluorescent focus assays.

Authors:  C E Stokes; J M Bernstein; S A Kyger; F G Hayden
Journal:  J Clin Microbiol       Date:  1988-07       Impact factor: 5.948

3.  Detection and serotyping of herpes simplex virus in MRC-5 cells by use of centrifugation and monoclonal antibodies 16 h postinoculation.

Authors:  C A Gleaves; D J Wilson; A D Wold; T F Smith
Journal:  J Clin Microbiol       Date:  1985-01       Impact factor: 5.948

4.  Rapid detection of influenza virus by shell vial assay with monoclonal antibodies.

Authors:  M J Espy; T F Smith; M W Harmon; A P Kendal
Journal:  J Clin Microbiol       Date:  1986-10       Impact factor: 5.948

5.  Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigen.

Authors:  C A Gleaves; T F Smith; E A Shuster; G R Pearson
Journal:  J Clin Microbiol       Date:  1984-06       Impact factor: 5.948

6.  Mechanisms of herpes simplex virus infectivity enhanced by ultracentrifugal inoculation.

Authors:  R B Tenser; M E Dunstan
Journal:  Infect Immun       Date:  1980-10       Impact factor: 3.441

7.  Rapid diagnosis of influenza infection of NP antigen using an immunocapture ELISA test.

Authors:  J J Chomel; D Thouvenot; M Onno; C Kaiser; J M Gourreau; M Aymard
Journal:  J Virol Methods       Date:  1989-07       Impact factor: 2.014

8.  Permanent canine kidney (MDCK) cells for isolation and plaque assay of influenza B viruses.

Authors:  K Tobita
Journal:  Med Microbiol Immunol       Date:  1975-12-30       Impact factor: 3.402

9.  Plaque assay and primary isolation of influenza A viruses in an established line of canine kidney cells (MDCK) in the presence of trypsin.

Authors:  K Tobita; A Sugiura; C Enomote; M Furuyama
Journal:  Med Microbiol Immunol       Date:  1975-12-30       Impact factor: 3.402

10.  One-incubation time-resolved fluoroimmunoassay based on monoclonal antibodies in detection of influenza A and B viruses directly in clinical specimens.

Authors:  S Nikkari; P Halonen; I Kharitonenkov; M Kivivirta; M Khristova; M Waris; A Kendal
Journal:  J Virol Methods       Date:  1989-01       Impact factor: 2.014
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  7 in total

1.  Evaluation of a new dot blot enzyme immunoassay (directigen flu A+B) for simultaneous and differential detection of influenza a and B virus antigens from respiratory samples.

Authors:  Jordi Reina; Emma Padilla; Fermin Alonso; Enrique Ruiz De Gopegui; Maria Munar; Margarita Mari
Journal:  J Clin Microbiol       Date:  2002-09       Impact factor: 5.948

2.  Comparison of Madin-Darby canine kidney cells (MDCK) with a green monkey continuous cell line (Vero) and human lung embryonated cells (MRC-5) in the isolation of influenza A virus from nasopharyngeal aspirates by shell vial culture.

Authors:  J Reina; V Fernandez-Baca; I Blanco; M Munar
Journal:  J Clin Microbiol       Date:  1997-07       Impact factor: 5.948

Review 3.  Physical and chemical methods for enhancing rapid detection of viruses and other agents.

Authors:  J H Hughes
Journal:  Clin Microbiol Rev       Date:  1993-04       Impact factor: 26.132

4.  Laboratory-based assessment of influenza in German ambulant patients from 1998 to 2008.

Authors:  E Terletskaia-Ladwig; M Eggers; S Meier; M Leinmüller; F Schneider; M Schmid; M Enders
Journal:  Infection       Date:  2009-08-07       Impact factor: 3.553

5.  Epidemiology of herpes simplex virus types 1 and 2 in Germany: what has changed?

Authors:  S Buxbaum; M Geers; G Gross; H Schöfer; H F Rabenau; H W Doerr
Journal:  Med Microbiol Immunol       Date:  2003-05-22       Impact factor: 3.402

Review 6.  New aspects of influenza viruses.

Authors:  M W Shaw; N H Arden; H F Maassab
Journal:  Clin Microbiol Rev       Date:  1992-01       Impact factor: 26.132

7.  Enhancement of feline infectious peritonitis virus type I infection in cell cultures using low-speed centrifugation.

Authors:  T Hohdatsu; T Tatekawa; H Koyama
Journal:  J Virol Methods       Date:  1995-02       Impact factor: 2.014
  7 in total

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