Literature DB >> 9090133

Selective 15N labeling and direct observation by NMR of the active-site glutamine of Fe-containing superoxide dismutase.

C K Vance1, Y M Kang, A F Miller.   

Abstract

The glutamine in position 69 is one of only three conserved active-site amino acid differences between Fe- and Mn-containing superoxide dismutases (SODs). We have refined the conditions for extremely selective labeling of the side chains of glutamine with 15N, and thus obtained dramatically simplified spectra, despite the large size of Fe-SOD. The improved resolution afforded by such highly specific labeling permits the use of direct 15N detection to observe and assign Gln 69, even though its distance to the paramagnetic Fe2+ is only 5 A. Selective glutamine side-chain labeling is inexpensive and has general utility for large (and paramagnet-containing) proteins.

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Year:  1997        PMID： 9090133     DOI： 10.1023/a:1018662421878

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  17 in total

1.  Physical and chemical studies on bacterial superoxide dismutases. Purification and some anion binding properties of the iron-containing protein of Escherichia coli B.

Authors:  T O Slykhouse; J A Fee
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2.  Expression and nitrogen-15 labeling of proteins for proton and nitrogen-15 nuclear magnetic resonance.

Authors:  D C Muchmore; L P McIntosh; C B Russell; D E Anderson; F W Dahlquist
Journal:  Methods Enzymol       Date:  1989       Impact factor: 1.600

3.  Iron- and manganese-containing superoxide dismutases can be distinguished by analysis of their primary structures.

Authors:  M W Parker; C C Blake
Journal:  FEBS Lett       Date:  1988-03-14       Impact factor: 4.124

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Authors:  H Cheng; J L Markley
Journal:  Annu Rev Biophys Biomol Struct       Date:  1995

5.  Manganese and iron superoxide dismutases are structural homologs.

Authors:  W C Stallings; K A Pattridge; R K Strong; M L Ludwig
Journal:  J Biol Chem       Date:  1984-09-10       Impact factor: 5.157

6.  Multinuclear magnetic resonance studies of the 2Fe.2S* ferredoxin from Anabaena species strain PCC 7120. 3. Detection and characterization of hyperfine-shifted nitrogen-15 and hydrogen-1 resonances of the oxidized form.

Authors:  B H Oh; J L Markley
Journal:  Biochemistry       Date:  1990-04-24       Impact factor: 3.162

7.  Differences between the manganese- and the iron-containing superoxide dismutases of Escherichia coli detected through sedimentation equilibrium, hydrodynamic, and spectroscopic studies.

Authors:  W F Beyer; J A Reynolds; I Fridovich
Journal:  Biochemistry       Date:  1989-05-16       Impact factor: 3.162

8.  Nuclear magnetic resonance studies of Rhodospirillum rubrum cytochrome c'.

Authors:  M H Emptage; A V Xavier; J M Wood; B M Alsaadi; G R Moore; R C Pitt; R J Williams; R P Ambler; R G Bartsch
Journal:  Biochemistry       Date:  1981-01-06       Impact factor: 3.162

9.  Structure-function in Escherichia coli iron superoxide dismutase: comparisons with the manganese enzyme from Thermus thermophilus.

Authors:  M S Lah; M M Dixon; K A Pattridge; W C Stallings; J A Fee; M L Ludwig
Journal:  Biochemistry       Date:  1995-02-07       Impact factor: 3.162

10.  The pH-dependent changes of the enzymic activity and spectroscopic properties of iron-substituted manganese superoxide dismutase. A study on the metal-specific activity of Mn-containing superoxide dismutase.

Authors:  F Yamakura; K Kobayashi; H Ue; M Konno
Journal:  Eur J Biochem       Date:  1995-02-01
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  7 in total

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Authors:  Maayan Gal; Katherine A Edmonds; Alexander G Milbradt; Koh Takeuchi; Gerhard Wagner
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2.  Nitrogen-detected CAN and CON experiments as alternative experiments for main chain NMR resonance assignments.

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Journal:  J Biomol NMR       Date:  2010-06-17       Impact factor: 2.835

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Authors:  D L Sorkin; A F Miller
Journal:  J Biomol NMR       Date:  2000-08       Impact factor: 2.835

Review 4.  Isotope labeling for solution and solid-state NMR spectroscopy of membrane proteins.

Authors:  Raffaello Verardi; Nathaniel J Traaseth; Larry R Masterson; Vitaly V Vostrikov; Gianluigi Veglia
Journal:  Adv Exp Med Biol       Date:  2012       Impact factor: 2.622

5.  15N-NMR characterization of His residues in and around the active site of FeSOD.

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Journal:  Biochim Biophys Acta       Date:  2009-11-18

6.  Selective (15)N-labeling of the side-chain amide groups of asparagine and glutamine for applications in paramagnetic NMR spectroscopy.

Authors:  Chan Cao; Jia-Liang Chen; Yin Yang; Feng Huang; Gottfried Otting; Xun-Cheng Su
Journal:  J Biomol NMR       Date:  2014-07-08       Impact factor: 2.835

7.  Asparagine and Glutamine Side-Chains and Ladders in HET-s(218-289) Amyloid Fibrils Studied by Fast Magic-Angle Spinning NMR.

Authors:  Thomas Wiegand; Alexander A Malär; Riccardo Cadalbert; Matthias Ernst; Anja Böckmann; Beat H Meier
Journal:  Front Mol Biosci       Date:  2020-09-30
  7 in total

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