Literature DB >> 9023958

A recombinase-mediated system for elimination of antibiotic resistance gene markers from genetically engineered Bacillus thuringiensis strains.

V Sanchis1, H Agaisse, J Chaufaux, D Lereclus.   

Abstract

A TnpI-mediated site-specific recombination system to construct genetically modified Bacillus thuringiensis strains was developed. Recombinant B. thuringiensis strains from which antibiotic resistance genes can be selectively eliminated were obtained in vivo with a new vector based on the specific resolution site of transposon Tn4430. For example, a cryIC gene, whose product is active against Spodoptera littoralis, was introduced into B. thuringiensis Kto harboring a cryIA(c) gene active against Ostrinia nubilalis. The resulting strain had a broader activity spectrum than that of the parental strain. It contained only B. thuringiensis DNA and was free of antibiotic resistance genes. This should facilitate regulatory approval for its development as a commercial biopesticide.

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Year:  1997        PMID： 9023958      PMCID： PMC168370          DOI： 10.1128/aem.63.2.779-784.1997

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  17 in total

Review 1.  Genetic manipulation of Bacillus thuringiensis insecticidal crystal protein genes in bacteria.

Authors:  C Gawron-Burke; J A Baum
Journal:  Genet Eng (N Y)       Date:  1991

2.  Construction of Novel Bacillus thuringiensis Strains with Different Insecticidal Activities by Transduction and Transformation.

Authors:  M M Lecadet; J Chaufaux; J Ribier; D Lereclus
Journal:  Appl Environ Microbiol       Date:  1992-03       Impact factor: 4.792

3.  Construction of cloning vectors for Bacillus thuringiensis.

Authors:  O Arantes; D Lereclus
Journal:  Gene       Date:  1991-12-01       Impact factor: 3.688

4.  Nucleotide sequence of the Streptococcus faecalis plasmid gene encoding the 3'5"-aminoglycoside phosphotransferase type III.

Authors:  P Trieu-Cuot; P Courvalin
Journal:  Gene       Date:  1983-09       Impact factor: 3.688

5.  Nucleotide sequence and analysis of the N-terminal coding region of the Spodoptera-active delta-endotoxin gene of Bacillus thuringiensis aizawai 7.29.

Authors:  V Sanchis; D Lereclus; G Menou; J Chaufaux; S Guo; M M Lecadet
Journal:  Mol Microbiol       Date:  1989-02       Impact factor: 3.501

6.  Transformation and expression of a cloned delta-endotoxin gene in Bacillus thuringiensis.

Authors:  D Lereclus; O Arantès; J Chaufaux; M Lecadet
Journal:  FEMS Microbiol Lett       Date:  1989-07-15       Impact factor: 2.742

7.  Characterization and comparative sequence analysis of replication origins from three large Bacillus thuringiensis plasmids.

Authors:  J A Baum; M P Gilbert
Journal:  J Bacteriol       Date:  1991-09       Impact factor: 3.490

8.  Antibiotic-resistance cassettes for Bacillus subtilis.

Authors:  A M Guérout-Fleury; K Shazand; N Frandsen; P Stragier
Journal:  Gene       Date:  1995-12-29       Impact factor: 3.688

9.  A comparison and analysis of the toxicity and receptor binding properties of Bacillus thuringiensis CryIC delta-endotoxin on Spodoptera littoralis and Bombyx mori.

Authors:  V Sanchis; J Chaufaux; D Pauron
Journal:  FEBS Lett       Date:  1994-10-24       Impact factor: 4.124

10.  Structural and functional analysis of Tn4430: identification of an integrase-like protein involved in the co-integrate-resolution process.

Authors:  J Mahillon; D Lereclus
Journal:  EMBO J       Date:  1988-05       Impact factor: 11.598
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  10 in total

1.  An efficient method of selectable marker gene excision by Xer recombination for gene replacement in bacterial chromosomes.

Authors:  Alexandra E Bloor; Rocky M Cranenburgh
Journal:  Appl Environ Microbiol       Date:  2006-04       Impact factor: 4.792

Review 2.  Recombinant entomopathogenic agents: a review of biotechnological approaches to pest insect control.

Authors:  Salih Karabörklü; Ugur Azizoglu; Zehra Busra Azizoglu
Journal:  World J Microbiol Biotechnol       Date:  2017-12-18       Impact factor: 3.312

3.  Mosquito larvicidal activity of transgenic Anabaena strain PCC 7120 expressing combinations of genes from Bacillus thuringiensis subsp. israelensis.

Authors:  W Xiaoqiang; S J Vennison; L Huirong; E Ben-Dov; A Zaritsky; S Boussiba
Journal:  Appl Environ Microbiol       Date:  1997-12       Impact factor: 4.792

4.  Development and field performance of a broad-spectrum nonviable asporogenic recombinant strain of Bacillus thuringiensis with greater potency and UV resistance.

Authors:  V Sanchis; M Gohar; J Chaufaux; O Arantes; A Meier; H Agaisse; J Cayley; D Lereclus
Journal:  Appl Environ Microbiol       Date:  1999-09       Impact factor: 4.792

Review 5.  Bacillus thuringiensis and its pesticidal crystal proteins.

Authors:  E Schnepf; N Crickmore; J Van Rie; D Lereclus; J Baum; J Feitelson; D R Zeigler; D H Dean
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

6.  Bacillus anthracis multiplication, persistence, and genetic exchange in the rhizosphere of grass plants.

Authors:  Elke Saile; Theresa M Koehler
Journal:  Appl Environ Microbiol       Date:  2006-05       Impact factor: 4.792

7.  Molecular characterization of a DNA fragment harboring the replicon of pBMB165 from Bacillus thuringiensis subsp. tenebrionis.

Authors:  Junyan Huang; Suxia Guo; Jacques Mahillon; Géraldine A Van der Auwera; Li Wang; Dongmei Han; Ziniu Yu; Ming Sun
Journal:  BMC Genomics       Date:  2006-10-23       Impact factor: 3.969

8.  Multiple integration of the gene ganA into the Bacillus subtilis chromosome for enhanced β-galactosidase production using the CRISPR/Cas9 system.

Authors:  Hildegard Watzlawick; Josef Altenbuchner
Journal:  AMB Express       Date:  2019-09-30       Impact factor: 3.298

Review 9.  Regulation of cry gene expression in Bacillus thuringiensis.

Authors:  Chao Deng; Qi Peng; Fuping Song; Didier Lereclus
Journal:  Toxins (Basel)       Date:  2014-07-23       Impact factor: 4.546

10.  Mob/oriT, a mobilizable site-specific recombination system for unmarked genetic manipulation in Bacillus thuringiensis and Bacillus cereus.

Authors:  Pengxia Wang; Yiguang Zhu; Yuyang Zhang; Chunyi Zhang; Jianyi Xu; Yun Deng; Donghai Peng; Lifang Ruan; Ming Sun
Journal:  Microb Cell Fact       Date:  2016-06-10       Impact factor: 5.328
  10 in total

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