| Literature DB >> 8566804 |
A M Guérout-Fleury1, K Shazand, N Frandsen, P Stragier.
Abstract
The genes encoding resistance to four different antibiotics (erythromycin, kanamycin, tetracycline and spectinomycin) were cloned in the polylinker of various Escherichia coli plasmid vectors. These cassettes can be inserted into cloned Bacillus subtilis (Bs) genes and used to create tagged chromosomal disruptions after recombination into Bs and selection in the presence of the appropriate antibiotic.Entities:
Mesh:
Year: 1995 PMID: 8566804 DOI: 10.1016/0378-1119(95)00652-4
Source DB: PubMed Journal: Gene ISSN: 0378-1119 Impact factor: 3.688