Literature DB >> 2550317

Transformation and expression of a cloned delta-endotoxin gene in Bacillus thuringiensis.

D Lereclus1, O Arantès, J Chaufaux, M Lecadet.   

Abstract

A shuttle vector containing the replication region of a resident plasmid of B. thuringiensis, was used to determine the conditions allowing efficient transformation of B. thuringiensis by electroporation. Using this plasmid a delta-endotoxin gene was cloned and expressed both in Escherichia coli and B. thuringiensis. It was shown that this gene was poorly expressed in the wild type situation whereas after cloning in acrystalliferous strains of B. thuringiensis large amounts of crystal protein were obtained.

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Year:  1989        PMID： 2550317     DOI： 10.1016/0378-1097(89)90511-9

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  153 in total

1.  Interaction between functional domains of Bacillus thuringiensis insecticidal crystal proteins.

Authors:  C Rang; V Vachon; R A de Maagd; M Villalon; J L Schwartz; D Bosch; R Frutos; R Laprade
Journal:  Appl Environ Microbiol       Date:  1999-07       Impact factor: 4.792

2.  Domain I plays an important role in the crystallization of Cry3A in Bacillus thuringiensis.

Authors:  H W Park; B A Federici
Journal:  Mol Biotechnol       Date:  2000-10       Impact factor: 2.695

3.  Regulation by overlapping promoters of the rate of synthesis and deposition into crystalline inclusions of Bacillus thuringiensis delta-endotoxins.

Authors:  M Sedlak; T Walter; A Aronson
Journal:  J Bacteriol       Date:  2000-02       Impact factor: 3.490

4.  Regulation of the acetoin catabolic pathway is controlled by sigma L in Bacillus subtilis.

Authors:  N O Ali; J Bignon; G Rapoport; M Debarbouille
Journal:  J Bacteriol       Date:  2001-04       Impact factor: 3.490

5.  A cell-cell signaling peptide activates the PlcR virulence regulon in bacteria of the Bacillus cereus group.

Authors:  Leyla Slamti; Didier Lereclus
Journal:  EMBO J       Date:  2002-09-02       Impact factor: 11.598

6.  Effect of specific mutations in helix alpha7 of domain I on the stability and crystallization of Cry3A in Bacillus thuringiensis.

Authors:  Hyun-Woo Park; Brian A Federici
Journal:  Mol Biotechnol       Date:  2004-06       Impact factor: 2.695

7.  Identification of the promoter in the intergenic region between orf1 and cry8Ea1 controlled by sigma H factor.

Authors:  Lixin Du; Lili Qiu; Qi Peng; Didier Lereclus; Jie Zhang; Fuping Song; Dafang Huang
Journal:  Appl Environ Microbiol       Date:  2012-04-13       Impact factor: 4.792

8.  Bacterial swimmers that infiltrate and take over the biofilm matrix.

Authors:  Ali Houry; Michel Gohar; Julien Deschamps; Ekaterina Tischenko; Stéphane Aymerich; Alexandra Gruss; Romain Briandet
Journal:  Proc Natl Acad Sci U S A       Date:  2012-07-05       Impact factor: 11.205

9.  Aedes aegypti alkaline phosphatase ALP1 is a functional receptor of Bacillus thuringiensis Cry4Ba and Cry11Aa toxins.

Authors:  Alan I Jiménez; Esmeralda Z Reyes; Angeles Cancino-Rodezno; Leidy P Bedoya-Pérez; Gustavo G Caballero-Flores; Luis F Muriel-Millan; Supaporn Likitvivatanavong; Sarjeet S Gill; Alejandra Bravo; Mario Soberón
Journal:  Insect Biochem Mol Biol       Date:  2012-06-20       Impact factor: 4.714

Review 10.  Bacillus thuringiensis and its pesticidal crystal proteins.

Authors:  E Schnepf; N Crickmore; J Van Rie; D Lereclus; J Baum; J Feitelson; D R Zeigler; D H Dean
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056
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