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Literature DB >> 9016663

Interaction of the RecA protein of Escherichia coli with single-stranded oligodeoxyribonucleotides.

Abstract

The RecA protein of Escherichia coli performs a number of ATP-dependent, in vitro reactions and is a DNA-dependent ATPase. Small oligodeoxyribonucleotides were used as DNA cofactors in a kinetic analysis of the ATPase reaction. Polymers of deoxythymidilic acid as well as oligonucleotides of mixed base composition stimulated the RecA ATPase activity in a length-dependent fashion. Both the initial rate and the extent of the reaction were affected by chain length. Full activity was seen with chain lengths > or = 30 nt. Partial activity was seen with chain lengths of 15-30 nt. The lower activity of shorter oligonucleotides was not simply due to a reduced affinity for DNA, since effects of chain length on KmATP and the Hill coefficient for ATP hydrolysis were also observed. The results also suggested that single-stranded DNA secondary structure frequently affects the ATPase activity of RecA protein with oligodeoxyribonucleotides.

Entities: Chemical Species

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Year: 1996 PMID： 9016663 PMCID： PMC146329 DOI： 10.1093/nar/24.24.4933

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

44 in total

1. Use of psoralen-modified oligonucleotides to trap three-stranded RecA-DNA complexes and repair of these cross-linked complexes by ABC excinuclease.

Authors: S Cheng; B Van Houten; H B Gamper; A Sancar; J E Hearst
Journal: J Biol Chem Date: 1988-10-15 Impact factor: 5.157

2. recA protein-promoted ATP hydrolysis occurs throughout recA nucleoprotein filaments.

Authors: S L Brenner; R S Mitchell; S W Morrical; S K Neuendorf; B C Schutte; M M Cox
Journal: J Biol Chem Date: 1987-03-25 Impact factor: 5.157

3. Structure of helical RecA-DNA complexes. Complexes formed in the presence of ATP-gamma-S or ATP.

Authors: E H Egelman; A Stasiak
Journal: J Mol Biol Date: 1986-10-20 Impact factor: 5.469

4. Role of homology and pathway specificity for recombination between plasmids and bacteriophage lambda.

Authors: S R King; J P Richardson
Journal: Mol Gen Genet Date: 1986-07

5. RecA protein-dependent proteolysis of bacteriophage lambda repressor Characterization of the reaction and stimulation by DNA-binding proteins.

Authors: G M Weinstock; K McEntee
Journal: J Biol Chem Date: 1981-11-10 Impact factor: 5.157

6. tif-1 mutation alters polynucleotide recognition by the recA protein of Escherichia coli.

Authors: K McEntee; G M Weinstock
Journal: Proc Natl Acad Sci U S A Date: 1981-10 Impact factor: 11.205

7. Interaction of recA protein with a photoaffinity analogue of ATP, 8-azido-ATP: determination of nucleotide cofactor binding parameters and of the relationship between ATP binding and ATP hydrolysis.

Authors: S C Kowalczykowski
Journal: Biochemistry Date: 1986-10-07 Impact factor: 3.162

8. Topography of the interaction of recA protein with single-stranded deoxyoligonucleotides.

Authors: M C Leahy; C M Radding
Journal: J Biol Chem Date: 1986-05-25 Impact factor: 5.157

9. recA protein of Escherichia coli promotes branch migration, a kinetically distinct phase of DNA strand exchange.

Authors: M M Cox; I R Lehman
Journal: Proc Natl Acad Sci U S A Date: 1981-06 Impact factor: 11.205

10. DNA sequence dependence of ATP hydrolysis by RecA protein.

Authors: M Amaratunga; A S Benight
Journal: Biochem Biophys Res Commun Date: 1988-11-30 Impact factor: 3.575

18 in total

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Authors: Yasushi Shigemori; Michio Oishi
Journal: Nucleic Acids Res Date: 2004-01-02 Impact factor: 16.971

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Authors: Yury V Kil; Eugene A Glazunov; Vladislav A Lanzov
Journal: J Bacteriol Date: 2005-04 Impact factor: 3.490

3. DNA helicase activity of PcrA is not required for the displacement of RecA protein from DNA or inhibition of RecA-mediated strand exchange.

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Journal: J Bacteriol Date: 2007-04-20 Impact factor: 3.490

4. Rad54 oligomers translocate and cross-bridge double-stranded DNA to stimulate synapsis.

Authors: Piero R Bianco; Justin J Bradfield; Lauren R Castanza; Andrea N Donnelly
Journal: J Mol Biol Date: 2007-09-22 Impact factor: 5.469

5. The intrinsically disordered linker of E. coli SSB is critical for the release from single-stranded DNA.

Authors: Hui Yin Tan; Luke A Wilczek; Sasheen Pottinger; Maria Manosas; Cong Yu; Trong Nguyenduc; Piero R Bianco
Journal: Protein Sci Date: 2017-03-08 Impact factor: 6.725

6. Using RecA protein to enhance kinetic rates of DNA circuits.

Authors: J N Milligan; A D Ellington
Journal: Chem Commun (Camb) Date: 2015-06-11 Impact factor: 6.222

7. Activation of the Vibrio cholerae SOS response is not required for intestinal cholera toxin production or colonization.

Authors: Mariam Quinones; Brigid M Davis; Matthew K Waldor
Journal: Infect Immun Date: 2006-02 Impact factor: 3.441