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Literature DB >> 17955055

Probing the structure of RecA-DNA filaments. Advantages of a fluorescent guanine analog.

Scott F Singleton¹, Alberto I Roca, Andrew M Lee, Jie Xiao.

Abstract

The RecA protein of Escherichia coli plays a crucial roles in DNA recombination and repair, as well as various aspects of bacterial pathogenicity. The formation of a RecA-ATP-ssDNA complex initiates all RecA activities and yet a complete structural and mechanistic description of this filament has remained elusive. An analysis of RecA-DNA interactions was performed using fluorescently labeled oligonucleotides. A direct comparison was made between fluorescein and several fluorescent nucleosides. The fluorescent guanine analog 6-methylisoxanthopterin (6MI) demonstrated significant advantages over the other fluorophores and represents an important new tool for characterizing RecA-DNA interactions.

Entities: Chemical Gene Species

Year: 2007 PMID： 17955055 PMCID： PMC2031864 DOI： 10.1016/j.tet.2006.10.092

Source DB: PubMed Journal: Tetrahedron ISSN： 0040-4020 Impact factor: 2.457

90 in total

1. Kinetic mechanism of the single-stranded DNA recognition by Escherichia coli replicative helicase DnaB protein. Application of the matrix projection operator technique to analyze stopped-flow kinetics.

Authors: W Bujalowski; M J Jezewska
Journal: J Mol Biol Date: 2000-01-28 Impact factor: 5.469

2. Elucidating a key intermediate in homologous DNA strand exchange: structural characterization of the RecA-triple-stranded DNA complex using fluorescence resonance energy transfer.

Authors: Jie Xiao; Scott F Singleton
Journal: J Mol Biol Date: 2002-07-12 Impact factor: 5.469

Review 3. Stress responses and genetic variation in bacteria.

Authors: Patricia L Foster
Journal: Mutat Res Date: 2005-01-06 Impact factor: 2.433

4. Second-site RecA-DNA interactions: lack of identical recognition.

Authors: P Wittung; L R Bazemore; M Takahashi; B Nordén; C Radding
Journal: Biochemistry Date: 1996-12-03 Impact factor: 3.162

5. Real time fluorescence analysis of the RecA filament: implications of base pair fluidity in repeat realignment.

Authors: S Sen; G Krishnamoorthy; B J Rao
Journal: FEBS Lett Date: 2001-03-02 Impact factor: 4.124

6. Studies of the interaction of RecA protein with DNA.

Authors: D F Dombroski; D G Scraba; R D Bradley; A R Morgan
Journal: Nucleic Acids Res Date: 1983-11-11 Impact factor: 16.971

7. Fluorescence properties of pteridine nucleoside analogs as monomers and incorporated into oligonucleotides.

Authors: M E Hawkins; W Pfleiderer; F M Balis; D Porter; J R Knutson
Journal: Anal Biochem Date: 1997-01-01 Impact factor: 3.365

8. Influence of 5'-nearest neighbors on the insertion kinetics of the fluorescent nucleotide analog 2-aminopurine by Klenow fragment.

Authors: L B Bloom; M R Otto; J M Beechem; M F Goodman
Journal: Biochemistry Date: 1993-10-19 Impact factor: 3.162

Review 9. Recombination, repair and replication in the pathogenic Neisseriae: the 3 R's of molecular genetics of two human-specific bacterial pathogens.

Authors: Kimberly A Kline; Eric V Sechman; Eric P Skaar; H Steven Seifert
Journal: Mol Microbiol Date: 2003-10 Impact factor: 3.501

Probing the structure of RecA-DNA filaments. Advantages of a fluorescent guanine analog.

1. Kinetic mechanism of the single-stranded DNA recognition by Escherichia coli replicative helicase DnaB protein. Application of the matrix projection operator technique to analyze stopped-flow kinetics.

2. Elucidating a key intermediate in homologous DNA strand exchange: structural characterization of the RecA-triple-stranded DNA complex using fluorescence resonance energy transfer.

Review 3. Stress responses and genetic variation in bacteria.

4. Second-site RecA-DNA interactions: lack of identical recognition.

5. Real time fluorescence analysis of the RecA filament: implications of base pair fluidity in repeat realignment.

6. Studies of the interaction of RecA protein with DNA.

7. Fluorescence properties of pteridine nucleoside analogs as monomers and incorporated into oligonucleotides.

8. Influence of 5'-nearest neighbors on the insertion kinetics of the fluorescent nucleotide analog 2-aminopurine by Klenow fragment.

Review 9. Recombination, repair and replication in the pathogenic Neisseriae: the 3 R's of molecular genetics of two human-specific bacterial pathogens.

10. Synthesis and hydrolysis of oligodeoxyribonucleotides containing 2-aminopurine.

1. A complementary pair of rapid molecular screening assays for RecA activities.

Review 2. Fluorescent analogs of biomolecular building blocks: design, properties, and applications.

3. Applying 6-methylisoxanthopterin-enhanced fluorescence to examine protein-DNA interactions in the picomolar range.

4. Hot-spot identification on a broad class of proteins and RNA suggest unifying principles of molecular recognition.

5. Conservation of a conformational switch in RadA recombinase from Methanococcus maripaludis.