Literature DB >> 8948434

Prediction and Fourier-transform infrared-spectroscopy estimation of the secondary structure of a recombinant beta-glucosidase from Streptomyces sp. (ATCC 11238).

J A Perez-Pons1, E Padros, E Querol.   

Abstract

The secondary structure of a recombinant beta-glucosidase (EC 3.2.1.21) from Streptomyces sp. (ATCC 11238) has been predicted by computer algorithms and also estimated by Fourier-transform IR spectroscopy. From curve fitting of the deconvoluted IR spectra, the most probable distribution of the secondary-structural classes appears to be about 34% alpha-helix, 30% beta-sheet, 25% reverse turns and 11% non-ordered structures. These data showed a good agreement with data from computer prediction (35% alpha-helix, 23% beta-sheet, 31% reverse turns and 11% non-ordered structures).

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Year:  1995        PMID： 8948434      PMCID： PMC1136794          DOI： 10.1042/bj3080791

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  36 in total

1.  A possible way for prediction of domain boundaries in globular proteins from amino acid sequence.

Authors:  F Vonderviszt; I Simon
Journal:  Biochem Biophys Res Commun       Date:  1986-08-29       Impact factor: 3.575

2.  Examination of the secondary structure of proteins by deconvolved FTIR spectra.

Authors:  D M Byler; H Susi
Journal:  Biopolymers       Date:  1986-03       Impact factor: 2.505

3.  The mechanism of enzymatic cellulose degradation. Isolation and some properties of a beta-glucosidase from Trichoderma viride.

Authors:  L E Berghem; L G Pettersson
Journal:  Eur J Biochem       Date:  1974-07-15

4.  An Excel spreadsheet computer program combining algorithms for prediction of protein structural characteristics.

Authors:  J Clotet; J Cedano; E Querol
Journal:  Comput Appl Biosci       Date:  1994-09

5.  Predicting protein function: a versatile tool for the Apple Macintosh.

Authors:  R Fuchs
Journal:  Comput Appl Biosci       Date:  1994-04

6.  Crystallization and preliminary X-ray diffraction analysis of a type I beta-glucosidase encoded by the bgIA gene of Bacillus polymyxa.

Authors:  J Sanz-Aparicio; A Romero; M Martinez-Ripoll; A Madarro; A Flors; J Polaina
Journal:  J Mol Biol       Date:  1994-07-15       Impact factor: 5.469

7.  A simple method for displaying the hydropathic character of a protein.

Authors:  J Kyte; R F Doolittle
Journal:  J Mol Biol       Date:  1982-05-05       Impact factor: 5.469

8.  A beta-glucosidase gene (bgl3) from Streptomyces sp. strain QM-B814. Molecular cloning, nucleotide sequence, purification and characterization of the encoded enzyme, a new member of family 1 glycosyl hydrolases.

Authors:  J A Perez-Pons; A Cayetano; X Rebordosa; J Lloberas; A Guasch; E Querol
Journal:  Eur J Biochem       Date:  1994-07-15

9.  Functional and structural role of a tryptophan generally observed in protein-carbohydrate interaction. TRP-62 of hen egg white lysozyme.

Authors:  K Maenaka; G Kawai; K Watanabe; F Sunada; I Kumagai
Journal:  J Biol Chem       Date:  1994-03-11       Impact factor: 5.157

Review 10.  The biological degradation of cellulose.

Authors:  P Béguin; J P Aubert
Journal:  FEMS Microbiol Rev       Date:  1994-01       Impact factor: 16.408
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