Literature DB >> 8028010

Crystallization and preliminary X-ray diffraction analysis of a type I beta-glucosidase encoded by the bgIA gene of Bacillus polymyxa.

J Sanz-Aparicio1, A Romero, M Martinez-Ripoll, A Madarro, A Flors, J Polaina.   

Abstract

The enzyme encoded by the bgIA gene of Bacillus polymyxa, a type I beta-glucosidase belonging to family I of glycosyl hydrolases, has been purified to homogeneity from an Escherichia coli culture which overexpressed the gene, and crystallized. The crystals, which diffract to 3.0 A resolution, belong to the orthorhombic space group C222(1). The cell dimensions are a = 155.4 A, b = 209.4 A, c = 209.7 A.

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Year:  1994        PMID: 8028010     DOI: 10.1006/jmbi.1994.1441

Source DB:  PubMed          Journal:  J Mol Biol        ISSN: 0022-2836            Impact factor:   5.469


  2 in total

1.  Prediction and Fourier-transform infrared-spectroscopy estimation of the secondary structure of a recombinant beta-glucosidase from Streptomyces sp. (ATCC 11238).

Authors:  J A Perez-Pons; E Padros; E Querol
Journal:  Biochem J       Date:  1995-06-15       Impact factor: 3.857

2.  Amino acid substitutions enhancing thermostability of Bacillus polymyxa beta-glucosidase A.

Authors:  C Lopez-Camacho; J Salgado; J L Lequerica; A Madarro; E Ballestar; L Franco; J Polaina
Journal:  Biochem J       Date:  1996-03-15       Impact factor: 3.857

  2 in total

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