Literature DB >> 8943759

Sensitive and universal method for microbial DNA extraction from blood products.

N Golbang1, J P Burnie, P E Klapper, A Bostock, P Williamson.   

Abstract

A new method of extracting bacterial and yeast DNA from blood products dependent on guanidinium thiocyanate acid extraction and proteinase K treatment is described. In spiked samples the sensitivity per 0.1 ml of serum and blood, respectively, was 26 and 150 colony forming units (cfu) for Escherichia coli, 80 and 120 cfu for Staphylococcus aureus and 20 and 26 cfu for Candida albicans. This compared well with existing methodologies, worked on limited clinical samples and was not pathogen specific.

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Year:  1996        PMID： 8943759      PMCID： PMC500787          DOI： 10.1136/jcp.49.10.861

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  21 in total

1.  New method for the extraction of viral RNA and DNA from cerebrospinal fluid for use in the polymerase chain reaction assay.

Authors:  I Casas; L Powell; P E Klapper; G M Cleator
Journal:  J Virol Methods       Date:  1995-05       Impact factor: 2.014

2.  Rapid detection of the mecA gene in methicillin-resistant staphylococci by enzymatic detection of polymerase chain reaction products.

Authors:  K Ubukata; S Nakagami; A Nitta; A Yamane; S Kawakami; M Sugiura; M Konno
Journal:  J Clin Microbiol       Date:  1992-07       Impact factor: 5.948

3.  New method for detection of Candida albicans in human blood by polymerase chain reaction.

Authors:  Y Miyakawa; T Mabuchi; Y Fukazawa
Journal:  J Clin Microbiol       Date:  1993-12       Impact factor: 5.948

4.  Major trends in the microbial etiology of nosocomial infection.

Authors:  D R Schaberg; D H Culver; R P Gaynes
Journal:  Am J Med       Date:  1991-09-16       Impact factor: 4.965

5.  Application of the polymerase chain reaction to the diagnosis of candidosis by amplification of an HSP 90 gene fragment.

Authors:  A C Crampin; R C Matthews
Journal:  J Med Microbiol       Date:  1993-09       Impact factor: 2.472

6.  Polymerase chain reaction for the diagnosis of candidemia.

Authors:  V L Kan
Journal:  J Infect Dis       Date:  1993-09       Impact factor: 5.226

7.  Evaluation of polymerase chain reaction for diagnosis of pneumococcal pneumonia.

Authors:  K M Rudolph; A J Parkinson; C M Black; L W Mayer
Journal:  J Clin Microbiol       Date:  1993-10       Impact factor: 5.948

8.  Detection of Salmonella typhi in the blood of patients with typhoid fever by polymerase chain reaction.

Authors:  J H Song; H Cho; M Y Park; D S Na; H B Moon; C H Pai
Journal:  J Clin Microbiol       Date:  1993-06       Impact factor: 5.948

9.  Peripheral-blood-based PCR assay to identify patients with active pulmonary tuberculosis.

Authors:  R Condos; A McClune; W N Rom; N W Schluger
Journal:  Lancet       Date:  1996-04-20       Impact factor: 79.321

10.  PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid.

Authors:  K Greisen; M Loeffelholz; A Purohit; D Leong
Journal:  J Clin Microbiol       Date:  1994-02       Impact factor: 5.948
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  3 in total

1.  Semiquantitative polymerase chain reaction enzyme immunoassay for diagnosis of disseminated candidiasis.

Authors:  J P Burnie; N Golbang; R C Matthews
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1997-05       Impact factor: 3.267

2.  Detection of bacteraemia in patients with fever and neutropenia using 16S rRNA gene amplification by polymerase chain reaction.

Authors:  B E Ley; C J Linton; D M Bennett; H Jalal; A B Foot; M R Millar
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1998-04       Impact factor: 3.267

3.  Development and validation of a flexible DNA extraction (PAN) method for liquid biopsy of multiple sample types.

Authors:  Xiao-Xia Chen; Wei Chen; Yi-Long Liu; Can-Xiang Lin; Mengmeng Li; Wen-Jie Chen; Shang-Hang Xie; Dong-Feng Lin; Su-Mei Cao
Journal:  J Clin Lab Anal       Date:  2021-08-16       Impact factor: 2.352
  3 in total

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