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Literature DB >> 8354923

Polymerase chain reaction for the diagnosis of candidemia.

Abstract

A PCR assay was developed for the diagnosis of candidemia. Primers were selected to amplify a 158-bp segment from the Candida actin gene that was detected by hybridization with a 32P-labeled oligomer probe. The lower limit of detection was DNA extracted from 10 Candida yeasts/sample, or 25 fg of purified Candida albicans DNA. This PCR was specific for medically important Candida species. Circulating Candida DNA was detected by this PCR from plasma of mice with induced candidemia and from sera in 11 (79%) of 14 patients with blood cultures positive for Candida species. This PCR may offer a sensitive method for diagnosis of candidemia.

Entities: Chemical Disease Species

Mesh：

Substances：
Actins
DNA, Fungal

Year: 1993 PMID： 8354923 DOI： 10.1093/infdis/168.3.779

Source DB: PubMed Journal: J Infect Dis ISSN： 0022-1899 Impact factor: 5.226

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Cited

47 in total

1. Seminested PCR for diagnosis of candidemia: comparison with culture, antigen detection, and biochemical methods for species identification.

Authors: Suhail Ahmad; Zaiba Khan; Abu S Mustafa; Zia U Khan
Journal: J Clin Microbiol Date: 2002-07 Impact factor: 5.948

2. Primers ITS1, ITS2 and ITS4 detect the intraspecies variability in the internal transcribed spacers and 5.8S rRNA gene region in clinical isolates of fungi.

Authors: M Korabecná; V Liska; K Fajfrlík
Journal: Folia Microbiol (Praha) Date: 2003 Impact factor: 2.099

3. Biochemical and molecular characterization of wild-type and fused protoplasts of Beauveria bassiana and Metarhizium anisopliae.

Authors: V Ranga; Gurvinder Kaur Saini
Journal: Folia Microbiol (Praha) Date: 2011-08-05 Impact factor: 2.099

4. Diagnosis of invasive candidiasis in neutropenic children with cancer by determination of D-arabinitol/L-arabinitol ratios in urine.

Authors: B Christensson; T Wiebe; C Pehrson; L Larsson
Journal: J Clin Microbiol Date: 1997-03 Impact factor: 5.948

5. Identification of various medically important Candida species in clinical specimens by PCR-restriction enzyme analysis.

Authors: G Morace; M Sanguinetti; B Posteraro; G Lo Cascio; G Fadda
Journal: J Clin Microbiol Date: 1997-03 Impact factor: 5.948

Polymerase chain reaction for the diagnosis of candidemia.

1. Seminested PCR for diagnosis of candidemia: comparison with culture, antigen detection, and biochemical methods for species identification.

2. Primers ITS1, ITS2 and ITS4 detect the intraspecies variability in the internal transcribed spacers and 5.8S rRNA gene region in clinical isolates of fungi.

3. Biochemical and molecular characterization of wild-type and fused protoplasts of Beauveria bassiana and Metarhizium anisopliae.

4. Diagnosis of invasive candidiasis in neutropenic children with cancer by determination of D-arabinitol/L-arabinitol ratios in urine.

5. Identification of various medically important Candida species in clinical specimens by PCR-restriction enzyme analysis.

6. Development of a DNA microarray for detection and identification of fungal pathogens involved in invasive mycoses.

7. Identification of contaminating fungal DNA sequences in Zymolyase.

8. PCR monitoring of response to liposomal amphotericin B treatment of systemic candidiasis in neutropenic mice.

9. Rapid identification of Candida species by DNA fingerprinting with PCR.

10. Improved detection of Candida albicans by PCR in blood of neutropenic mice with systemic candidiasis.