| Literature DB >> 8308135 |
Y Miyakawa1, T Mabuchi, Y Fukazawa.
Abstract
We developed a new method for detection of Candida albicans cells in human blood by the polymerase chain reaction that amplifies a 125-bp region within a species-specific, multiple-copy DNA fragment. In preparing template DNA from C. albicans-containing blood, the addition of C. tropicalis cells, together with antibody which reacts with both cells, raised the sensitivity of detection of C. albicans to about 3 cells per 0.1 ml.Entities:
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Year: 1993 PMID: 8308135 PMCID: PMC266430 DOI: 10.1128/jcm.31.12.3344-3347.1993
Source DB: PubMed Journal: J Clin Microbiol ISSN: 0095-1137 Impact factor: 5.948