Literature DB >> 8308135

New method for detection of Candida albicans in human blood by polymerase chain reaction.

Y Miyakawa1, T Mabuchi, Y Fukazawa.   

Abstract

We developed a new method for detection of Candida albicans cells in human blood by the polymerase chain reaction that amplifies a 125-bp region within a species-specific, multiple-copy DNA fragment. In preparing template DNA from C. albicans-containing blood, the addition of C. tropicalis cells, together with antibody which reacts with both cells, raised the sensitivity of detection of C. albicans to about 3 cells per 0.1 ml.

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Year:  1993        PMID: 8308135      PMCID: PMC266430          DOI: 10.1128/jcm.31.12.3344-3347.1993

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  14 in total

Review 1.  The polymerase chain reaction. A new method of using molecular genetics for medical diagnosis.

Authors:  B I Eisenstein
Journal:  N Engl J Med       Date:  1990-01-18       Impact factor: 91.245

2.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors:  R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

3.  Clinical comparison of the recoveries of bloodstream pathogens in Septi-Chek brain heart infusion broth with saponin, Septi-Chek tryptic soy broth, and the isolator lysis-centrifugation system.

Authors:  P R Murray; A W Spizzo; A C Niles
Journal:  J Clin Microbiol       Date:  1991-05       Impact factor: 5.948

Review 4.  Genetics of Candida albicans.

Authors:  S Scherer; P T Magee
Journal:  Microbiol Rev       Date:  1990-09

5.  Clinical comparison of the resin-containing BACTEC 26 Plus and the Isolator 10 blood culturing systems.

Authors:  J J Tarrand; C Guillot; M Wenglar; J Jackson; J D Lajeunesse; K V Rolston
Journal:  J Clin Microbiol       Date:  1991-10       Impact factor: 5.948

6.  Role of specific determinants in mannan of Candida albicans serotype A in adherence to human buccal epithelial cells.

Authors:  Y Miyakawa; T Kuribayashi; K Kagaya; M Suzuki; T Nakase; Y Fukazawa
Journal:  Infect Immun       Date:  1992-06       Impact factor: 3.441

7.  Production and characterization of agglutinating monoclonal antibodies against predominant antigenic factors for Candida albicans.

Authors:  Y Miyakawa; K Kagaya; Y Fukazawa; G Soe
Journal:  J Clin Microbiol       Date:  1986-05       Impact factor: 5.948

Review 8.  The use of molecular techniques for epidemiologic typing of Candida species.

Authors:  M A Pfaller
Journal:  Curr Top Med Mycol       Date:  1992

9.  High frequency variation of colony morphology and chromosome reorganization in the pathogenic yeast Candida albicans.

Authors:  T Suzuki; I Kobayashi; T Kanbe; K Tanaka
Journal:  J Gen Microbiol       Date:  1989-02

10.  Application of DNA typing methods to Candida albicans epidemiology and correlations with phenotype.

Authors:  D A Stevens; F C Odds; S Scherer
Journal:  Rev Infect Dis       Date:  1990 Mar-Apr
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  27 in total

1.  Seminested PCR for diagnosis of candidemia: comparison with culture, antigen detection, and biochemical methods for species identification.

Authors:  Suhail Ahmad; Zaiba Khan; Abu S Mustafa; Zia U Khan
Journal:  J Clin Microbiol       Date:  2002-07       Impact factor: 5.948

2.  Detection of Candida species by nested PCR method in one-humped camels (Camelus dromedarius).

Authors:  Ugur Parin; Goksel Erbas; Sukru Kirkan; Serap Savasan; H Tugba Yuksel; Gamze Balat
Journal:  Trop Anim Health Prod       Date:  2017-10-28       Impact factor: 1.559

3.  Identification of contaminating fungal DNA sequences in Zymolyase.

Authors:  D Rimek; A P Garg; W H Haas; R Kappe
Journal:  J Clin Microbiol       Date:  1999-03       Impact factor: 5.948

4.  PCR monitoring of response to liposomal amphotericin B treatment of systemic candidiasis in neutropenic mice.

Authors:  A J van Deventer; W H Goessens; A van Belkum; E W van Etten; H J van Vliet; H A Verbrugh
Journal:  J Clin Microbiol       Date:  1996-01       Impact factor: 5.948

5.  Rapid identification of Candida species by DNA fingerprinting with PCR.

Authors:  M Thanos; G Schonian; W Meyer; C Schweynoch; Y Graser; T G Mitchell; W Presber; H J Tietz
Journal:  J Clin Microbiol       Date:  1996-03       Impact factor: 5.948

6.  Semiquantitative polymerase chain reaction enzyme immunoassay for diagnosis of disseminated candidiasis.

Authors:  J P Burnie; N Golbang; R C Matthews
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1997-05       Impact factor: 3.267

7.  Sensitive and universal method for microbial DNA extraction from blood products.

Authors:  N Golbang; J P Burnie; P E Klapper; A Bostock; P Williamson
Journal:  J Clin Pathol       Date:  1996-10       Impact factor: 3.411

8.  Rapid detection of Candida albicans in clinical samples by DNA amplification of common regions from C. albicans-secreted aspartic proteinase genes.

Authors:  M Flahaut; D Sanglard; M Monod; J Bille; M Rossier
Journal:  J Clin Microbiol       Date:  1998-02       Impact factor: 5.948

9.  Reverse cross blot hybridization assay for rapid detection of PCR-amplified DNA from candida species, Cryptococcus neoformans, and Saccharomyces cerevisiae in clinical samples.

Authors:  B Posteraro; M Sanguinetti; L Masucci; L Romano; G Morace; G Fadda
Journal:  J Clin Microbiol       Date:  2000-04       Impact factor: 5.948

10.  Improved detection of Candida albicans by PCR in blood of neutropenic mice with systemic candidiasis.

Authors:  A J van Deventer; W H Goessens; A van Belkum; H J van Vliet; E W van Etten; H A Verbrugh
Journal:  J Clin Microbiol       Date:  1995-03       Impact factor: 5.948

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