Literature DB >> 8939024

A novel polymerase chain reaction assay for the detection and speciation of thermophilic Campylobacter spp.

C J Jackson1, A J Fox, D M Jones.   

Abstract

A novel PCR amplification method is described which is specific for the thermophilic, enteropathogenic species Campylobacter jejuni, Camp. coli and Camp. upsaliensis. Rapid, accurate speciation of amplified strains is possible on the basis of restriction fragment length polymorphisms of PCR products digested with three restriction enzymes, AluI, DdeI and DraI. The sensitivity of detection is 25 cfu in water, and 2 x 10(3) cfu in full cream milk. An epidemiological application of the assay in detecting non-culturable campylobacters from a contaminated potable water supply is described.

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Year:  1996        PMID： 8939024     DOI： 10.1111/j.1365-2672.1996.tb03534.x

Source DB:  PubMed          Journal:  J Appl Bacteriol        ISSN： 0021-8847


  13 in total

Review 1.  Inhibition and facilitation of nucleic acid amplification.

Authors:  I G Wilson
Journal:  Appl Environ Microbiol       Date:  1997-10       Impact factor: 4.792

2.  A real-time PCR assay for the detection of Campylobacter jejuni in foods after enrichment culture.

Authors:  Andrew D Sails; Andrew J Fox; Frederick J Bolton; David R A Wareing; David L A Greenway
Journal:  Appl Environ Microbiol       Date:  2003-03       Impact factor: 4.792

3.  Prevalence of Campylobacter spp., Escherichia coli, and Salmonella serovars in retail chicken, turkey, pork, and beef from the Greater Washington, D.C., area.

Authors:  C Zhao; B Ge; J De Villena; R Sudler; E Yeh; S Zhao; D G White; D Wagner; J Meng
Journal:  Appl Environ Microbiol       Date:  2001-12       Impact factor: 4.792

4.  Detection of small numbers of Campylobacter jejuni and Campylobacter coli cells in environmental water, sewage, and food samples by a seminested PCR assay.

Authors:  A S Waage; T Vardund; V Lund; G Kapperud
Journal:  Appl Environ Microbiol       Date:  1999-04       Impact factor: 4.792

5.  Rapid identification of thermotolerant Campylobacter jejuni, Campylobacter coli, Campylobacter lari, and Campylobacter upsaliensis from various geographic locations by a GTPase-based PCR-reverse hybridization assay.

Authors:  L J van Doorn; A Verschuuren-van Haperen; A Burnens; M Huysmans; P Vandamme; B A Giesendorf; M J Blaser; W G Quint
Journal:  J Clin Microbiol       Date:  1999-06       Impact factor: 5.948

6.  Specific PCR identification and differentiation of the thermophilic campylobacters, Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis.

Authors:  C Fermér; E O Engvall
Journal:  J Clin Microbiol       Date:  1999-10       Impact factor: 5.948

7.  Development of an indirect competitive ELISA for detection of Campylobacter jejuni subsp.jejuni O:23 in foods.

Authors:  I Hochel; D Viochna; J Skvor; M Musil
Journal:  Folia Microbiol (Praha)       Date:  2004       Impact factor: 2.099

8.  Detection of Campylobacter jejuni and Campylobacter coli in environmental waters by PCR enzyme-linked immunosorbent assay.

Authors:  A D Sails; F J Bolton; A J Fox; D R A Wareing; D L A Greenway
Journal:  Appl Environ Microbiol       Date:  2002-03       Impact factor: 4.792

9.  Sandwich assay for mixed-sequence recognition of double-stranded DNA: invader-based detection of targets specific to foodborne pathogens.

Authors:  Benjamin Denn; Saswata Karmakar; Dale C Guenther; Patrick J Hrdlicka
Journal:  Chem Commun (Camb)       Date:  2013-10-28       Impact factor: 6.222

10.  Genotypic identification of erythromycin-resistant campylobacter isolates as helicobacter species and analysis of resistance mechanism.

Authors:  Ed J Kuijper; Servi Stevens; Toshihiro Imamura; Bob De Wever; Eric C J Claas
Journal:  J Clin Microbiol       Date:  2003-08       Impact factor: 5.948
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