Literature DB >> 10488210

Specific PCR identification and differentiation of the thermophilic campylobacters, Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis.

C Fermér1, E O Engvall.   

Abstract

A sensitive PCR assay that detects the thermophilic campylobacters C. jejuni, C. coli, C. lari, and C. upsaliensis is reported. Furthermore, by digestion of the PCR products with two restriction enzymes, species differentiation was demonstrated. Thus, the present method has the potential to be used for both detection and identification of thermophilic Campylobacter species.

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Year:  1999        PMID： 10488210      PMCID： PMC85572          DOI： 10.1128/JCM.37.10.3370-3373.1999

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  19 in total

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2.  The 23S ribosomal RNA higher-order structure of Pseudomonas cepacia and other prokaryotes.

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3.  Amplification and sequencing of variable regions in bacterial 23S ribosomal RNA genes with conserved primer sequences.

Authors:  G Van Camp; S Chapelle; R De Wachter
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4.  Direct polymerase chain reaction detection of Campylobacter jejuni and Campylobacter coli in raw milk and dairy products.

Authors:  B Wegmüller; J Lüthy; U Candrian
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5.  Rapid and sensitive detection of Campylobacter spp. in chicken products by using the polymerase chain reaction.

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Journal:  Appl Environ Microbiol       Date:  1992-12       Impact factor: 4.792

6.  Discrimination among thermophilic Campylobacter species by polymerase chain reaction amplification of 23S rRNA gene fragments.

Authors:  M Eyers; S Chapelle; G Van Camp; H Goossens; R De Wachter
Journal:  J Clin Microbiol       Date:  1993-12       Impact factor: 5.948

7.  Phylogenetic and molecular characterization of a 23S rRNA gene positions the genus Campylobacter in the epsilon subdivision of the Proteobacteria and shows that the presence of transcribed spacers is common in Campylobacter spp.

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9.  Genome maps of Campylobacter jejuni and Campylobacter coli.

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Journal:  J Clin Microbiol       Date:  1995-04       Impact factor: 5.948
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  32 in total

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7.  The internal transcribed spacer region, a new tool for use in species differentiation and delineation of systematic relationships within the Campylobacter genus.

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Review 8.  Global Epidemiology of Campylobacter Infection.

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9.  Multilocus sequence typing of Campylobacter jejuni isolates from humans, chickens, raw milk, and environmental water in Quebec, Canada.

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10.  Metagenomic diagnosis of bacterial infections.

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