Literature DB >> 8880529

Rapid preparation of bacterial DNA for pulsed-field gel electrophoresis.

M G Matushek1, M J Bonten, M K Hayden.   

Abstract

A disadvantage of genotyping bacterial strains by pulsed-field gel electrophoresis is that the procedure requires up to 6 days to complete. We modified a standard pulsed-field gel electrophoresis method (B.E. Murray, K.V. Singh, J.D. Health, B.R. Sharma, and G.M. Weinstock, J.Clin. Microbiol. 28:2059-2063, 1990) so that it could be completed in less than 3 days. We successfully applied this method to the analysis of a variety of gram-positive and gram-negative bacteria.

Mesh：

Substances：

Year:  1996        PMID： 8880529      PMCID： PMC229328          DOI： 10.1128/jcm.34.10.2598-2600.1996

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  Rapid method for epidemiological evaluation of gram-positive cocci by field inversion gel electrophoresis.

Authors:  R V Goering; M A Winters
Journal:  J Clin Microbiol       Date:  1992-03       Impact factor: 5.948

2.  Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites.

Authors:  B E Murray; K V Singh; J D Heath; B R Sharma; G M Weinstock
Journal:  J Clin Microbiol       Date:  1990-09       Impact factor: 5.948

Review 3.  Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing.

Authors:  F C Tenover; R D Arbeit; R V Goering; P A Mickelsen; B E Murray; D H Persing; B Swaminathan
Journal:  J Clin Microbiol       Date:  1995-09       Impact factor: 5.948

Review 4.  Molecular epidemiology: application of contemporary techniques to the typing of microorganisms.

Authors:  J N Maslow; M E Mulligan; R D Arbeit
Journal:  Clin Infect Dis       Date:  1993-08       Impact factor: 9.079

Review 5.  Molecular epidemiology of nosocomial infection: analysis of chromosomal restriction fragment patterns by pulsed-field gel electrophoresis.

Authors:  R V Goering
Journal:  Infect Control Hosp Epidemiol       Date:  1993-10       Impact factor: 3.254

6.  Pulsed-field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus.

Authors:  T L Bannerman; G A Hancock; F C Tenover; J M Miller
Journal:  J Clin Microbiol       Date:  1995-03       Impact factor: 5.948

7.  Comparison of MIDI Sherlock system and pulsed-field gel electrophoresis in characterizing strains of methicillin-resistant Staphylococcus aureus from a recent hospital outbreak.

Authors:  R B Leonard; J Mayer; M Sasser; M L Woods; B R Mooney; B G Brinton; P L Newcomb-Gayman; K C Carroll
Journal:  J Clin Microbiol       Date:  1995-10       Impact factor: 5.948
  7 in total
  52 in total

1.  Molecular characterization of multidrug-resistant Streptococcus pneumoniae isolates in Korea. The Asian Network for Surveillance of Resistant Pathogens (ANSORP) Study Group.

Authors:  J H Song; J W Yang; J H Jin; S W Kim; C K Kim; H Lee; K R Peck; S Kim; N Y Lee; M R Jacobs; P C Appelbaum
Journal:  J Clin Microbiol       Date:  2000-04       Impact factor: 5.948

2.  Availability of Nagarse for DNA analysis as a substitute for proteinase K.

Authors:  M Yokota; I Tsuda; N Tatsumi
Journal:  J Clin Lab Anal       Date:  2000       Impact factor: 2.352

3.  Macrorestriction fingerprinting of "Streptococcus milleri" group bacteria by pulsed-field gel electrophoresis.

Authors:  K L Bartie; M J Wilson; D W Williams; M A Lewis
Journal:  J Clin Microbiol       Date:  2000-06       Impact factor: 5.948

4.  Improved pulsed-field gel electrophoresis for typing vancomycin-resistant enterococci.

Authors:  D Turabelidze; M Kotetishvili; A Kreger; J G Morris; A Sulakvelidze
Journal:  J Clin Microbiol       Date:  2000-11       Impact factor: 5.948

5.  Rapid pulsed-field gel electrophoresis method for group B streptococcus isolates.

Authors:  J A Benson; P Ferrieri
Journal:  J Clin Microbiol       Date:  2001-08       Impact factor: 5.948

6.  Genetic features of Streptococcus agalactiae strains causing severe neonatal infections, as revealed by pulsed-field gel electrophoresis and hylB gene analysis.

Authors:  K Rolland; C Marois; V Siquier; B Cattier; R Quentin
Journal:  J Clin Microbiol       Date:  1999-06       Impact factor: 5.948

7.  Comparison of pulsed-field gel electrophoresis and coagulase gene restriction profile analysis techniques in the molecular typing of Staphylococcus aureus.

Authors:  C S Chiou; H L Wei; L C Yang
Journal:  J Clin Microbiol       Date:  2000-06       Impact factor: 5.948

8.  Dissemination of multiple drug resistance genes by class 1 integrons in Klebsiella pneumoniae isolates from four countries: a comparative study.

Authors:  Piklu Roy Chowdhury; Ana Ingold; Natasha Vanegas; Elena Martínez; John Merlino; Andrea Karina Merkier; Mercedes Castro; Gerardo González Rocha; Graciela Borthagaray; Daniela Centrón; Helia Bello Toledo; Carolina M Márquez; H W Stokes
Journal:  Antimicrob Agents Chemother       Date:  2011-04-25       Impact factor: 5.191

9.  Clinical isolates of Enterobacteriaceae producing extended-spectrum beta-lactamases: prevalence of CTX-M-3 at a hospital in China.

Authors:  Hui Wang; Swathi Kelkar; Weiyuan Wu; Minjun Chen; John P Quinn
Journal:  Antimicrob Agents Chemother       Date:  2003-02       Impact factor: 5.191

10.  Molecular characteristics of nosocomial and Native American community-associated methicillin-resistant Staphylococcus aureus clones from rural Wisconsin.

Authors:  Sanjay K Shukla; Mary E Stemper; Srinivas V Ramaswamy; Jennifer M Conradt; Robert Reich; Edward A Graviss; Kurt D Reed
Journal:  J Clin Microbiol       Date:  2004-08       Impact factor: 5.948
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.