Literature DB >> 1313045

Rapid method for epidemiological evaluation of gram-positive cocci by field inversion gel electrophoresis.

R V Goering1, M A Winters.   

Abstract

We report a rapid method for the isolation of intact chromosomal DNA from gram-positive cocci that is suitable for in situ restriction endonuclease digestion in agarose blocks. When combined with a rapid field inversion gel electrophoresis protocol, this approach allows the preparation and electrophoretic analysis of chromosomal restriction fragments produced by rare-cutting enzymes in a total time period of 2 days from start to finish. The utility of the method is demonstrated in the epidemiological evaluation of Staphylococcus epidermidis clusters from two hospitals as well as of additional representative staphylococci and enterococci.

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Year:  1992        PMID： 1313045      PMCID： PMC265112          DOI： 10.1128/jcm.30.3.577-580.1992

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  Unexpected loss of genomic DNA from agarose gel plugs.

Authors:  R B Fritz; P R Musich
Journal:  Biotechniques       Date:  1990-11       Impact factor: 1.993

2.  DNA fingerprinting by pulsed field gel electrophoresis and ribotyping to distinguish Pseudomonas cepacia isolates from a nosocomial outbreak.

Authors:  D J Anderson; J S Kuhns; M L Vasil; D N Gerding; E N Janoff
Journal:  J Clin Microbiol       Date:  1991-03       Impact factor: 5.948

3.  Use of pulsed field gel electrophoresis to differentiate Coxiella burnetii strains.

Authors:  R Heinzen; G L Stiegler; L L Whiting; S A Schmitt; L P Mallavia; M E Frazier
Journal:  Ann N Y Acad Sci       Date:  1990       Impact factor: 5.691

4.  Rapid method for preparation and cleavage of bacterial DNA for pulsed-field gel electrophoresis.

Authors:  J L Flanagan; L Ventra; A S Weiss
Journal:  Nucleic Acids Res       Date:  1989-01-25       Impact factor: 16.971

5.  DNA polymorphism in Mycobacterium paratuberculosis, "wood pigeon mycobacteria," and related mycobacteria analyzed by field inversion gel electrophoresis.

Authors:  V V Lévy-Frébault; M F Thorel; A Varnerot; B Gicquel
Journal:  J Clin Microbiol       Date:  1989-12       Impact factor: 5.948

6.  A rapid and cost-effective method for preparing genomic DNA from gram-negative bacteria in agarose plugs for pulsed-field gel electrophoresis.

Authors:  B W Sobral; A G Atherly
Journal:  Biotechniques       Date:  1989-10       Impact factor: 1.993

7.  Purification, specific fragmentation, and separation of large DNA molecules.

Authors:  C L Smith; C R Cantor
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

8.  Electrophoretic separations of large DNA molecules by periodic inversion of the electric field.

Authors:  G F Carle; M Frank; M V Olson
Journal:  Science       Date:  1986-04-04       Impact factor: 47.728

9.  Resolution of recent evolutionary divergence among Escherichia coli from related lineages: the application of pulsed field electrophoresis to molecular epidemiology.

Authors:  R D Arbeit; M Arthur; R Dunn; C Kim; R K Selander; R Goldstein
Journal:  J Infect Dis       Date:  1990-02       Impact factor: 5.226

10.  Staphylococcus aureus in patients with cystic fibrosis: an epidemiological analysis using a combination of traditional and molecular methods.

Authors:  R V Goering; A Bauernfeind; W Lenz; B Przyklenk
Journal:  Infection       Date:  1990 Jan-Feb       Impact factor: 3.553
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  57 in total

1.  Rapid pulsed-field gel electrophoresis method for group B streptococcus isolates.

Authors:  J A Benson; P Ferrieri
Journal:  J Clin Microbiol       Date:  2001-08       Impact factor: 5.948

2.  Molecular characterization of vancomycin-resistant Enterococci repopulating the gastrointestinal tract following treatment with a novel glycolipodepsipeptide, ramoplanin.

Authors:  L R Baden; I A Critchley; D F Sahm; W So; M Gedde; S Porter; R C Moellering; G Eliopoulos
Journal:  J Clin Microbiol       Date:  2002-04       Impact factor: 5.948

3.  Comparison of pulsed-field gel electrophoresis and PCR analysis of polymorphisms on the mec hypervariable region for typing methicillin-resistant Staphylococcus aureus.

Authors:  J P M Senna; C A Pinto; L P S Carvalho; D S Santos
Journal:  J Clin Microbiol       Date:  2002-06       Impact factor: 5.948

4.  Staphylococcus aureus isolates with reduced susceptibility to glycopeptides belong to accessory gene regulator group I or II.

Authors:  Isabelle Verdier; Marie-Elisabeth Reverdy; Jerome Etienne; Gérard Lina; Michèle Bes; François Vandenesch
Journal:  Antimicrob Agents Chemother       Date:  2004-03       Impact factor: 5.191

5.  Hypervariability of biofilm formation and oxacillin resistance in a Staphylococcus epidermidis strain causing persistent severe infection in an immunocompromised patient.

Authors:  Maja Weisser; Sonja M K Schoenfelder; Christina Orasch; Caroline Arber; Alois Gratwohl; Reno Frei; Martin Eckart; Ursula Flückiger; Wilma Ziebuhr
Journal:  J Clin Microbiol       Date:  2010-05-26       Impact factor: 5.948

6.  A Panton-Valentine leucocidin (PVL)-positive community-acquired methicillin-resistant Staphylococcus aureus (MRSA) strain, another such strain carrying a multiple-drug resistance plasmid, and other more-typical PVL-negative MRSA strains found in Japan.

Authors:  Yoko Takizawa; Ikue Taneike; Saori Nakagawa; Tomohiro Oishi; Yoshiyuki Nitahara; Nobuhiro Iwakura; Kyoko Ozaki; Misao Takano; Teruko Nakayama; Tatsuo Yamamoto
Journal:  J Clin Microbiol       Date:  2005-07       Impact factor: 5.948

7.  Molecular characteristics and in vitro susceptibility to antimicrobial agents, including the des-fluoro(6) quinolone DX-619, of Panton-Valentine leucocidin-positive methicillin-resistant Staphylococcus aureus isolates from the community and hospitals.

Authors:  Tatsuo Yamamoto; Soshi Dohmae; Kohei Saito; Taketo Otsuka; Tomomi Takano; Megumi Chiba; Katsuko Fujikawa; Mayumi Tanaka
Journal:  Antimicrob Agents Chemother       Date:  2006-10-16       Impact factor: 5.191

8.  Carriage of community-associated methicillin-resistant Staphylococcus aureus in a cohort of infants in southern Israel: risk factors and molecular features.

Authors:  Amos Adler; Noga Givon-Lavi; Allon E Moses; Colin Block; Ron Dagan
Journal:  J Clin Microbiol       Date:  2009-12-09       Impact factor: 5.948

9.  The pls gene found in methicillin-resistant Staphylococcus aureus strains is common in clinical isolates of Staphylococcus sciuri.

Authors:  Katri Juuti; Salha Ibrahem; Anni Virolainen-Julkunen; Jaana Vuopio-Varkila; Pentti Kuusela
Journal:  J Clin Microbiol       Date:  2005-03       Impact factor: 5.948

10.  Pulsed-field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus.

Authors:  T L Bannerman; G A Hancock; F C Tenover; J M Miller
Journal:  J Clin Microbiol       Date:  1995-03       Impact factor: 5.948
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