Literature DB >> 8815098

Rapid identification of mycobacterial species by PCR amplification of hypervariable 16S rRNA gene promoter region.

P Dobner1, K Feldmann, M Rifai, T Löscher, H Rinder.   

Abstract

A total of 0.3 to 0.4 kb of the promoter region of the 16S rRNA genes from Mycobacterium tuberculosis, M. gordonae, M. xenopi, and M. leprae was PCR amplified, cloned, and sequenced. The observed number of substitutions, insertions, and deletions exceeded those found in previously used target sequences, including the entire 16S coding region. A simple and generally applicable restriction fragment length polymorphism method that can be used to distinguish between mycobacterial species is described.

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Year:  1996        PMID： 8815098      PMCID： PMC228907          DOI： 10.1128/jcm.34.4.866-869.1996

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  24 in total

1.  The division between fast- and slow-growing species corresponds to natural relationships among the mycobacteria.

Authors:  D A Stahl; J W Urbance
Journal:  J Bacteriol       Date:  1990-01       Impact factor: 3.490

2.  Nucleotide sequence of the 16S rRNA from Mycobacterium leprae.

Authors:  W Liesack; C Pitulle; S Sela; E Stackebrandt
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3.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

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4.  CLUSTAL: a package for performing multiple sequence alignment on a microcomputer.

Authors:  D G Higgins; P M Sharp
Journal:  Gene       Date:  1988-12-15       Impact factor: 3.688

5.  Structure of 5S rRNA in actinomycetes and relatives and evolution of eubacteria.

Authors:  E Dams; T Yamada; R De Baere; E Huysmans; A Vandenberghe; R De Wachter
Journal:  J Mol Evol       Date:  1987       Impact factor: 2.395

6.  Detection and identification of mycobacteria by amplification of rRNA.

Authors:  B Böddinghaus; T Rogall; T Flohr; H Blöcker; E C Böttger
Journal:  J Clin Microbiol       Date:  1990-08       Impact factor: 5.948

7.  Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium.

Authors:  T Rogall; J Wolters; T Flohr; E C Böttger
Journal:  Int J Syst Bacteriol       Date:  1990-10

8.  Rapid Mycobacterium species assignment and unambiguous identification of mutations associated with antimicrobial resistance in Mycobacterium tuberculosis by automated DNA sequencing.

Authors:  V Kapur; L L Li; M R Hamrick; B B Plikaytis; T M Shinnick; A Telenti; W R Jacobs; A Banerjee; S Cole; K Y Yuen
Journal:  Arch Pathol Lab Med       Date:  1995-02       Impact factor: 5.534

9.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  Molecular-genetic evidence for the relationship of Mycobacterium leprae to slow-growing pathogenic mycobacteria.

Authors:  J Smida; J Kazda; E Stackebrandt
Journal:  Int J Lepr Other Mycobact Dis       Date:  1988-09
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  9 in total

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Journal:  Appl Environ Microbiol       Date:  2010-09-17       Impact factor: 4.792

2.  Computational approach involving use of the internal transcribed spacer 1 region for identification of Mycobacterium species.

Authors:  Amr M Mohamed; Dan J Kuyper; Peter C Iwen; Hesham H Ali; Dhundy R Bastola; Steven H Hinrichs
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3.  Identification of nontuberculous mycobacteria using multilocous sequence analysis of 16S rRNA, hsp65, and rpoB.

Authors:  Si Hyun Kim; Jeong Hwan Shin
Journal:  J Clin Lab Anal       Date:  2017-02-23       Impact factor: 2.352

4.  Identification and Characterization of Mycobacterial Species Using Whole-Genome Sequences.

Authors:  Marco A Riojas; Andrew M Frank; Samuel R Greenfield; Stephen P King; Conor J Meehan; Michael Strong; Alice R Wattam; Manzour Hernando Hazbón
Journal:  Methods Mol Biol       Date:  2021

5.  Rapid identification of Mycobacteria to the species level using INNO-LiPA Mycobacteria, a reverse hybridization assay.

Authors:  P N Suffys; A da Silva Rocha; M de Oliveira; C E Campos; A M Barreto; F Portaels; L Rigouts; G Wouters; G Jannes; G van Reybroeck; W Mijs; B Vanderborght
Journal:  J Clin Microbiol       Date:  2001-12       Impact factor: 5.948

6.  Diagnostic value of PCR in genitourinary tuberculosis.

Authors:  Narotam Sharma; Veena Sharma; Prem Raj Singh; Shivani Sailwal; Rajeev S Kushwaha; Rajesh K Singh; Satish C Nautiyal; Pankaj Mishra; Tariq Masood; R K Singh
Journal:  Indian J Clin Biochem       Date:  2012-12-06

7.  Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of cultured mycobacteria in a diagnostic laboratory.

Authors:  Thierry De Baere; Ricardo de Mendonça; Geert Claeys; Gerda Verschraegen; Wouter Mijs; Rita Verhelst; Sylvianne Rottiers; Leen Van Simaey; Catharine De Ganck; Mario Vaneechoutte
Journal:  BMC Microbiol       Date:  2002-03-01       Impact factor: 3.605

8.  Computational identification of composite regulatory sites in 16s-rRNA gene promoters of Mycobacterium species.

Authors:  Neeraja Dwivedi; Surya Prakash Dwivedi; Ajay Kumar; Vishwa Mohan Katoch; Sanjay Mishra
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9.  A simple and efficient multiplex PCR assay for the identification of Mycobacterium genus and Mycobacterium tuberculosis complex to the species level.

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Journal:  Yonsei Med J       Date:  2013-09       Impact factor: 2.759
  9 in total

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