| Literature DB >> 34235665 |
Marco A Riojas1, Andrew M Frank2, Samuel R Greenfield2, Stephen P King2, Conor J Meehan3, Michael Strong4, Alice R Wattam5, Manzour Hernando Hazbón6.
Abstract
Whole-genome sequencing (WGS) has shown immense value in enabling identification and characterization of bacterial taxa. This is particularly true for mycobacteria, where culture-based characterization becomes delayed by the inherently slow growth rate of these organisms. This chapter reviews the general techniques behind WGS and their optimization, existing techniques for species-level identification and the advantages of WGS for this purpose, and a variety of useful tools for the genomic characterization of mycobacterial strains.Keywords: ANI; DNA; Mycobacteria; Sequencing; Species identification; Whole-genome sequence; dDDH
Year: 2021 PMID: 34235665 DOI: 10.1007/978-1-0716-1460-0_19
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745