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Literature DB >> 8795242

Use of green fluorescent protein to monitor survival of genetically engineered bacteria in aquatic environments.

Abstract

Many methods for detecting model genetically engineered microorganisms (GEMs) in experimental ecosystems rely on cultivation of introduced cells. In this study, survival of Escherichia coli was monitored with the green fluorescent protein (GFP) gene. This approach allowed enumeration of GEMs by both plating and microscopy. Use of the GFP-marked GEMs revealed that E. coli persisted in stream water at higher densities as determined microscopically than as determined by CFU enumeration. The GFP gene did not negatively impact the fitness of the host strain.

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Year: 1996 PMID： 8795242 PMCID： PMC168148 DOI： 10.1128/aem.62.9.3486-3488.1996

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

6 in total

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6 in total

14 in total

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Journal: Microb Ecol Date: 2001-02 Impact factor: 4.552

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6. Use of green fluorescent protein to tag and investigate gene expression in marine bacteria.

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