Literature DB >> 8779601

Genetic manipulation of the pathway for diacetyl metabolism in Lactococcus lactis.

S R Swindell1, K H Benson, H G Griffin, P Renault, S D Ehrlich, M J Gasson.   

Abstract

Diacetyl is an important food flavor compound produced by certain strains of citrate-metabolizing lactic acid bacteria. Citrate is converted to pyruvate, from which diacetyl is produced via intermediate alpha-acetolactate. This paper reports the cloning and analysis of the gene (aldB) encoding alpha-acetolactate decarboxylase from Lactococcus lactis MG1363. Deletion of the MG1363 chromosomal aldB gene was achieved by double crossover homologous recombination. The mutant strain was found to produce diacetyl; alpha-acetolactate decarboxylase activity was eliminated. Overexpression of the cloned ilvBN genes (encoding an alpha-acetolactate synthase) in the aldB deletion strain produced even higher levels of alpha-acetolactate, acetoin, and diacetyl.

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Year:  1996        PMID： 8779601      PMCID： PMC168044          DOI： 10.1128/aem.62.7.2641-2643.1996

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  12 in total

1.  Isolation, characterization, and physiological role of the pyruvate dehydrogenase complex and alpha-acetolactate synthase of Lactococcus lactis subsp. lactis bv. diacetylactis.

Authors:  J L Snoep; M J Teixeira de Mattos; M J Starrenburg; J Hugenholtz
Journal:  J Bacteriol       Date:  1992-07       Impact factor: 3.490

2.  Citrate Fermentation by Lactococcus and Leuconostoc spp.

Authors:  M J Starrenburg; J Hugenholtz
Journal:  Appl Environ Microbiol       Date:  1991-12       Impact factor: 4.792

3.  Effect of Initial Oxygen Concentration on Diacetyl and Acetoin Production by Lactococcus lactis subsp. lactis biovar diacetylactis.

Authors:  N Bassit; C Y Boquien; D Picque; G Corrieu
Journal:  Appl Environ Microbiol       Date:  1993-06       Impact factor: 4.792

4.  Isolation and characterization of Streptococcus cremoris Wg2-specific promoters.

Authors:  J M van der Vossen; D van der Lelie; G Venema
Journal:  Appl Environ Microbiol       Date:  1987-10       Impact factor: 4.792

5.  Imbalance of leucine flux in Lactococcus lactis and its use for the isolation of diacetyl-overproducing strains.

Authors:  N Goupil; G Corthier; S D Ehrlich; P Renault
Journal:  Appl Environ Microbiol       Date:  1996-07       Impact factor: 4.792

6.  Rapid isolation of genes from bacterial lambda libraries by direct polymerase chain reaction screening.

Authors:  H G Griffin; K J I'Anson; M J Gasson
Journal:  FEMS Microbiol Lett       Date:  1993-08-15       Impact factor: 2.742

7.  Gene inactivation in Lactococcus lactis: branched-chain amino acid biosynthesis.

Authors:  J J Godon; C Delorme; J Bardowski; M C Chopin; S D Ehrlich; P Renault
Journal:  J Bacteriol       Date:  1993-07       Impact factor: 3.490

8.  Branched-chain amino acid biosynthesis genes in Lactococcus lactis subsp. lactis.

Authors:  J J Godon; M C Chopin; S D Ehrlich
Journal:  J Bacteriol       Date:  1992-10       Impact factor: 3.490

9.  Metabolic operons in Lactococci.

Authors:  P Renault; J J Godon; N Goupil; C Delorme; G Corthier; S D Ehrlich
Journal:  Dev Biol Stand       Date:  1995

10.  Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing.

Authors:  M J Gasson
Journal:  J Bacteriol       Date:  1983-04       Impact factor: 3.490
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  19 in total

1.  Lactococcus lactis as a cell factory for high-level diacetyl production.

Authors:  J Hugenholtz; M Kleerebezem; M Starrenburg; J Delcour; W de Vos; P Hols
Journal:  Appl Environ Microbiol       Date:  2000-09       Impact factor: 4.792

2.  Analytical method validation and monitoring of diacetyl in liquors from Korean market.

Authors:  Hyeon-Hwa Lee; Ki-Teak Lee; Jung-Ah Shin
Journal:  Food Sci Biotechnol       Date:  2017-07-13       Impact factor: 2.391

3.  Isolation and properties of Lactococcus lactis subsp. lactis biovar diacetylactis CNRZ 483 mutants producing diacetyl and acetoin from glucose.

Authors:  H Boumerdassi; C Monnet; M Desmazeaud; G Corrieu
Journal:  Appl Environ Microbiol       Date:  1997-06       Impact factor: 4.792

4.  Cloning, expression, and characterization of the Lactococcus lactis pfl gene, encoding pyruvate formate-lyase.

Authors:  J Arnau; F Jørgensen; S M Madsen; A Vrang; H Israelsen
Journal:  J Bacteriol       Date:  1997-09       Impact factor: 3.490

5.  Imbalance of leucine flux in Lactococcus lactis and its use for the isolation of diacetyl-overproducing strains.

Authors:  N Goupil; G Corthier; S D Ehrlich; P Renault
Journal:  Appl Environ Microbiol       Date:  1996-07       Impact factor: 4.792

6.  Diacetyl and acetoin production from whey permeate using engineered Lactobacillus casei.

Authors:  Inmaculada Nadal; Juan Rico; Gaspar Pérez-Martínez; María J Yebra; Vicente Monedero
Journal:  J Ind Microbiol Biotechnol       Date:  2009-07-16       Impact factor: 3.346

7.  IS981-mediated adaptive evolution recovers lactate production by ldhB transcription activation in a lactate dehydrogenase-deficient strain of Lactococcus lactis.

Authors:  Roger S Bongers; Marcel H N Hoefnagel; Marjo J C Starrenburg; Marco A J Siemerink; John G A Arends; Jeroen Hugenholtz; Michiel Kleerebezem
Journal:  J Bacteriol       Date:  2003-08       Impact factor: 3.490

8.  Development and Use of a Screening Procedure for Production of (alpha)-Acetolactate by Lactococcus lactis subsp. lactis biovar diacetylactis Strains.

Authors:  C Monnet; P Schmitt; C Divies
Journal:  Appl Environ Microbiol       Date:  1997-02       Impact factor: 4.792

9.  Increased production of folate by metabolic engineering of Lactococcus lactis.

Authors:  Wilbert Sybesma; Marjo Starrenburg; Michiel Kleerebezem; Igor Mierau; Willem M de Vos; Jeroen Hugenholtz
Journal:  Appl Environ Microbiol       Date:  2003-06       Impact factor: 4.792

10.  Cofactor engineering: a novel approach to metabolic engineering in Lactococcus lactis by controlled expression of NADH oxidase.

Authors:  F Lopez de Felipe; M Kleerebezem; W M de Vos; J Hugenholtz
Journal:  J Bacteriol       Date:  1998-08       Impact factor: 3.490
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