Literature DB >> 1624471

Isolation, characterization, and physiological role of the pyruvate dehydrogenase complex and alpha-acetolactate synthase of Lactococcus lactis subsp. lactis bv. diacetylactis.

J L Snoep1, M J Teixeira de Mattos, M J Starrenburg, J Hugenholtz.   

Abstract

The pyruvate dehydrogenase complex of Lactococcus lactis subsp. lactis bv. diacetylactis has a specific activity of 6.6 U/mg and a Km of 1 mM for pyruvate. The specific activities of E2 and E3 in the complex are 30 and 0.36 U/mg, respectively. The complex is very sensitive to NADH inhibition and consists of four subunits: E1 alpha (44 kDa), E1 beta (35 kDa), E2 (73 kDa), and E3 (60 kDa). The L. lactis alpha-acetolactate synthase has a specific activity of 103 U/mg and a Km of 50 mM for pyruvate. Thiamine pyrophosphate (Km = 3.2 microM) and divalent cations are essential for activity. The native enzyme measures 172 kDa and consists of 62-kDa monomers. The role of both enzymes in product formation is discussed in view of NADH inhibition and competition for pyruvate.

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Year:  1992        PMID： 1624471      PMCID： PMC206284          DOI： 10.1128/jb.174.14.4838-4841.1992

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  18 in total

1.  Synthesis and properties of 2-acetylthiamin pyrophosphate: an enzymatic reaction intermediate.

Authors:  K J Gruys; C J Halkides; P A Frey
Journal:  Biochemistry       Date:  1987-12-01       Impact factor: 3.162

2.  Degradative acetolactate synthase of Bacillus subtilis: purification and properties.

Authors:  W D Holtzclaw; L F Chapman
Journal:  J Bacteriol       Date:  1975-03       Impact factor: 3.490

3.  Involvement of pyruvate dehydrogenase in product formation in pyruvate-limited anaerobic chemostat cultures of Enterococcus faecalis NCTC 775.

Authors:  J L Snoep; M J Teixeira de Mattos; P W Postma; O M Neijssel
Journal:  Arch Microbiol       Date:  1990       Impact factor: 2.552

4.  Regulation of the activity of the pyruvate dehydrogenase complex of Escherichia coli.

Authors:  E R Schwartz; L J Reed
Journal:  Biochemistry       Date:  1970-03-17       Impact factor: 3.162

5.  Alpha-keto acid dehydrogenase complexes. XII. Effects of acetylation on the activity and structure of the dihydrolipoyl transacetylase of Escherichia coli.

Authors:  E R Schwartz; L J Reed
Journal:  J Biol Chem       Date:  1969-11-25       Impact factor: 5.157

6.  The pH 6 acetolactate-forming enzyme from Serratia marcescens. Purification and properties.

Authors:  D Malthe-Sorenssen; F C Stormer
Journal:  Eur J Biochem       Date:  1970-05-01

7.  Oligomeric forms of plant acetolactate synthase depend on flavin adenine dinucleotide.

Authors:  J Durner; P Böger
Journal:  Plant Physiol       Date:  1990-07       Impact factor: 8.340

8.  Isolation and characterisation of the pyruvate dehydrogenase complex of anaerobically grown Enterococcus faecalis NCTC 775.

Authors:  J L Snoep; A H Westphal; J A Benen; M J Teixeira de Mattos; O M Neijssel; A de Kok
Journal:  Eur J Biochem       Date:  1992-01-15

9.  Lipoamide dehydrogenase from Azotobacter vinelandii. Molecular cloning, organization and sequence analysis of the gene.

Authors:  A H Westphal; A de Kok
Journal:  Eur J Biochem       Date:  1988-03-01

10.  A common structural motif in thiamin pyrophosphate-binding enzymes.

Authors:  C F Hawkins; A Borges; R N Perham
Journal:  FEBS Lett       Date:  1989-09-11       Impact factor: 4.124
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  40 in total

1.  Metabolic behavior of Lactococcus lactis MG1363 in microaerobic continuous cultivation at a low dilution rate.

Authors:  N B Jensen; C R Melchiorsen; K V Jokumsen; J Villadsen
Journal:  Appl Environ Microbiol       Date:  2001-06       Impact factor: 4.792

2.  Lactococcus lactis as a cell factory for high-level diacetyl production.

Authors:  J Hugenholtz; M Kleerebezem; M Starrenburg; J Delcour; W de Vos; P Hols
Journal:  Appl Environ Microbiol       Date:  2000-09       Impact factor: 4.792

3.  The pdh operon is expressed in a subpopulation of stationary-phase bacteria and is important for survival of sugar-starved Streptococcus mutans.

Authors:  Monica Busuioc; Bettina A Buttaro; Patrick J Piggot
Journal:  J Bacteriol       Date:  2010-06-25       Impact factor: 3.490

4.  Pyruvate fermentation by Oenococcus oeni and Leuconostoc mesenteroides and role of pyruvate dehydrogenase in anaerobic fermentation.

Authors:  Nicole Wagner; Quang Hon Tran; Hanno Richter; Paul M Selzer; Gottfried Unden
Journal:  Appl Environ Microbiol       Date:  2005-09       Impact factor: 4.792

5.  Differential expression of proteins and genes in the lag phase of Lactococcus lactis subsp. lactis grown in synthetic medium and reconstituted skim milk.

Authors:  Nadja Larsen; Mette Boye; Henrik Siegumfeldt; Mogens Jakobsen
Journal:  Appl Environ Microbiol       Date:  2006-02       Impact factor: 4.792

Review 6.  Transcription factor Rex in regulation of pathophysiology in oral pathogens.

Authors:  J P Bitoun; Z T Wen
Journal:  Mol Oral Microbiol       Date:  2015-08-06       Impact factor: 3.563

7.  Growth and Energy Generation by Lactococcus lactis subsp. lactis biovar diacetylactis during Citrate Metabolism.

Authors:  J Hugenholtz; L Perdon; T Abee
Journal:  Appl Environ Microbiol       Date:  1993-12       Impact factor: 4.792

8.  C Nuclear Magnetic Resonance Studies of Citrate and Glucose Cometabolism by Lactococcus lactis.

Authors:  A Ramos; K N Jordan; T M Cogan; H Santos
Journal:  Appl Environ Microbiol       Date:  1994-06       Impact factor: 4.792

9.  Cloning of the Lactococcus lactis adhE gene, encoding a multifunctional alcohol dehydrogenase, by complementation of a fermentative mutant of Escherichia coli.

Authors:  J Arnau; F Jørgensen; S M Madsen; A Vrang; H Israelsen
Journal:  J Bacteriol       Date:  1998-06       Impact factor: 3.490

10.  Diacetyl and acetoin production from whey permeate using engineered Lactobacillus casei.

Authors:  Inmaculada Nadal; Juan Rico; Gaspar Pérez-Martínez; María J Yebra; Vicente Monedero
Journal:  J Ind Microbiol Biotechnol       Date:  2009-07-16       Impact factor: 3.346
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