Literature DB >> 8405949

Rapid isolation of genes from bacterial lambda libraries by direct polymerase chain reaction screening.

H G Griffin1, K J I'Anson, M J Gasson.   

Abstract

A method for the direct screening of bacterial lambda libraries by polymerase chain reaction technology has been developed. This technique permits the identification and isolation of specific DNA sequences without the need for any filter hybridisation or radioactive probing. This strategy has been used to isolate a gene encoding lactate dehydrogenase from a Lactococcus lactis lambda library.

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Year:  1993        PMID： 8405949     DOI： 10.1111/j.1574-6968.1993.tb06422.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  7 in total

1.  Genetic manipulation of the pathway for diacetyl metabolism in Lactococcus lactis.

Authors:  S R Swindell; K H Benson; H G Griffin; P Renault; S D Ehrlich; M J Gasson
Journal:  Appl Environ Microbiol       Date:  1996-07       Impact factor: 4.792

2.  Inactivation of the glutamate decarboxylase gene in Lactococcus lactis subsp. cremoris.

Authors:  M Nomura; M Kobayashi; S Ohmomo; T Okamoto
Journal:  Appl Environ Microbiol       Date:  2000-05       Impact factor: 4.792

3.  Catabolite repression in Lactobacillus casei ATCC 393 is mediated by CcpA.

Authors:  V Monedero; M J Gosalbes; G Pérez-Martínez
Journal:  J Bacteriol       Date:  1997-11       Impact factor: 3.490

4.  Catabolism of branched-chain alpha-keto acids in Enterococcus faecalis: the bkd gene cluster, enzymes, and metabolic route.

Authors:  D E Ward; R P Ross; C C van der Weijden; J L Snoep; A Claiborne
Journal:  J Bacteriol       Date:  1999-09       Impact factor: 3.490

5.  Structural and functional analysis of the gene cluster encoding the enzymes of the arginine deiminase pathway of Lactobacillus sake.

Authors:  M Zúñiga; M Champomier-Verges; M Zagorec; G Pérez-Martínez
Journal:  J Bacteriol       Date:  1998-08       Impact factor: 3.490

6.  The isolation of Ant1, a transposable element from Aspergillus niger.

Authors:  D C Glayzer; I N Roberts; D B Archer; R P Oliver
Journal:  Mol Gen Genet       Date:  1995-12-10

7.  Genetic aspects of aromatic amino acid biosynthesis in Lactococcus lactis.

Authors:  H G Griffin; M J Gasson
Journal:  Mol Gen Genet       Date:  1995-01-06
  7 in total

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