Literature DB >> 8644926

Attomole-sensitivity electrospray source for large-molecule mass spectrometry.

G A Valaskovic1, N L Kelleher, D P Little, D J Aaserud, F W McLafferty.   

Abstract

Full mass spectra of high resolving power are obtained from 0.2 nL sample volumes of large (> 10 kDa) nucleotides and proteins using a new electrospray ionization (ESI) system combined with Fourier transform mass spectrometry. The ESI needles are fabricated by laser-heated pulling of fused-silica tubing (5-20 microns i.d.), followed by chemical etching and surface metalization. Total analyte loaded at the instrument of 8.6 fmol and 216 amol produces signal-to-noise ratios of 400:1 and 60:1, respectively, and resolving power of > 10(5) for full mass spectra, while the total amount of material consumed is approximately 150 and 10 amol, respectively.

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Year:  1995        PMID： 8644926     DOI： 10.1021/ac00116a030

Source DB:  PubMed          Journal:  Anal Chem        ISSN： 0003-2700            Impact factor:   6.986


  40 in total

1.  Sheathless preconcentration-capillary zone electrophoresis-mass spectrometry applied to peptide analysis.

Authors:  M B Barroso; A P de Jong
Journal:  J Am Soc Mass Spectrom       Date:  1999-12       Impact factor: 3.109

2.  Magnetic field focused ion accumulation for an internal bore liquid chromatography electrospray ionization Fourier transform ion cyclotron resonance mass spectrometer using a central trapping electrode

Authors: 
Journal:  J Am Soc Mass Spectrom       Date:  2000-06       Impact factor: 3.109

3.  Initial implementation of an electrodynamic ion funnel with Fourier transform ion cyclotron resonance mass spectrometry.

Authors:  M E Belov; M V Gorshkov; H R Udseth; G A Anderson; A V Tolmachev; D C Prior; R Harkewicz; R D Smith
Journal:  J Am Soc Mass Spectrom       Date:  2000-01       Impact factor: 3.109

4.  A high pressure matrix-assisted laser desorption ion source for Fourier transform mass spectrometry designed to accommodate large targets with diverse surfaces.

Authors:  Peter B O'Connor; Bogdan A Budnik; Vera B Ivleva; Parminder Kaur; Susanne C Moyer; Jason L Pittman; Catherine E Costello
Journal:  J Am Soc Mass Spectrom       Date:  2004-01       Impact factor: 3.109

5.  Automated orthogonal control system for electrospray ionization.

Authors:  Gary A Valaskovic; James P Murphy; Mike S Lee
Journal:  J Am Soc Mass Spectrom       Date:  2004-08       Impact factor: 3.109

6.  Reliable determinations of protein-ligand interactions by direct ESI-MS measurements. Are we there yet?

Authors:  Elena N Kitova; Amr El-Hawiet; Paul D Schnier; John S Klassen
Journal:  J Am Soc Mass Spectrom       Date:  2012-01-21       Impact factor: 3.109

7.  A study of electrospray ionization emitters with differing geometries with respect to flow rate and electrospray voltage.

Authors:  Brent R Reschke; Aaron T Timperman
Journal:  J Am Soc Mass Spectrom       Date:  2011-10-12       Impact factor: 3.109

8.  Trap for MAbs: characterization of intact monoclonal antibodies using reversed-phase HPLC on-line with ion-trap mass spectrometry.

Authors:  John C Le; Pavel V Bondarenko
Journal:  J Am Soc Mass Spectrom       Date:  2005-03       Impact factor: 3.109

9.  Characterization of a new qQq-FTICR mass spectrometer for post-translational modification analysis and top-down tandem mass spectrometry of whole proteins.

Authors:  Judith A Jebanathirajah; Jason L Pittman; Bruce A Thomson; Bogdan A Budnik; Parminder Kaur; Michael Rape; Marc Kirschner; Catherine E Costello; Peter B O'Connor
Journal:  J Am Soc Mass Spectrom       Date:  2005-11-02       Impact factor: 3.109

10.  An open design microfabricated nib-like nanoelectrospray emitter tip on a conducting silicon substrate for the application of the ionization voltage.

Authors:  Séverine Le Gac; Christian Rolando; Steve Arscott
Journal:  J Am Soc Mass Spectrom       Date:  2005-12-15       Impact factor: 3.109
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