Literature DB >> 8643608

The replication initiator protein pi of the plasmid R6K specifically interacts with the host-encoded helicase DnaB.

P V Ratnakar1, B K Mohanty, M Lobert, D Bastia.   

Abstract

The replication initiator protein pi of plasmid R6K is known to interact with the seven iterons of the gamma origin/enhancer and activate distant replication origins alpha and beta (ori alpha and ori beta) by pi-mediated DNA looping. Here we show that pi protein specifically interacts in vitro with the host-encoded helicase DnaB. The site of interaction of pi on DnaB has been localized to a 37-aa-long region located between amino acids 151 and 189 of DnaB. The surface of pi that interacts with DnaB has been mapped to the N-terminal region of the initiator protein between residues 1 and 116. The results suggest that during initiation of replication, the replicative helicase DnaB is first recruited to the gamma enhancer by the pi protein. In a subsequent step, the helicase probably gets delivered from ori gamma to ori alpha and ori beta by pi-mediated DNA looping.

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Year:  1996        PMID： 8643608      PMCID： PMC39279          DOI： 10.1073/pnas.93.11.5522

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  27 in total

1.  Molecular cloning of replication and incompatibility regions from the R-plasmid R6K.

Authors:  J H Crosa; L K Luttropp; S Falkow
Journal:  J Mol Biol       Date:  1978-09-25       Impact factor: 5.469

2.  Interaction of Escherichia coli dnaB and dnaC(D) gene products in vitro.

Authors:  S Wickner; J Hurwitz
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205

3.  Multiple pathways of copy control of gamma replicon of R6K: mechanisms both dependent on and independent of cooperativity of interaction of tau protein with DNA affect the copy number.

Authors:  A Miron; I Patel; D Bastia
Journal:  Proc Natl Acad Sci U S A       Date:  1994-07-05       Impact factor: 11.205

4.  Activity in vitro of three replication origins of the antibiotic resistance plasmid RSF1040.

Authors:  N Inuzuka; M Inuzuka; D R Helinski
Journal:  J Biol Chem       Date:  1980-12-10       Impact factor: 5.157

5.  The replication initiator protein of plasmid R6K tagged with beta-galactosidase shows sequence-specific DNA-binding.

Authors:  J Germino; D Bastia
Journal:  Cell       Date:  1983-01       Impact factor: 41.582

6.  Structural and functional studies of the dnaB protein using limited proteolysis. Characterization of domains for DNA-dependent ATP hydrolysis and for protein association in the primosome.

Authors:  N Nakayama; N Arai; Y Kaziro; K Arai
Journal:  J Biol Chem       Date:  1984-01-10       Impact factor: 5.157

7.  Structural properties of the beta origin of replication of plasmid R6K.

Authors:  A Shafferman; D R Helinski
Journal:  J Biol Chem       Date:  1983-04-10       Impact factor: 5.157

8.  Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K.

Authors:  R Kolter; M Inuzuka; D R Helinski
Journal:  Cell       Date:  1978-12       Impact factor: 41.582

9.  The nucleotide sequence of the replication origin beta of the plasmid R6K.

Authors:  M Shon; J Germino; D Bastia
Journal:  J Biol Chem       Date:  1982-11-25       Impact factor: 5.157

10.  Interaction of the plasmid R6K-encoded replication initiator protein with its binding sites on DNA.

Authors:  J Germino; D Bastia
Journal:  Cell       Date:  1983-08       Impact factor: 41.582
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  20 in total

1.  Mechanism of termination of DNA replication of Escherichia coli involves helicase-contrahelicase interaction.

Authors:  S Mulugu; A Potnis; J Taylor; K Alexander; D Bastia
Journal:  Proc Natl Acad Sci U S A       Date:  2001-08-07       Impact factor: 11.205

Review 2.  Strategies for helicase recruitment and loading in bacteria.

Authors:  Igor Konieczny
Journal:  EMBO Rep       Date:  2003-01       Impact factor: 8.807

3.  DnaK dependence of the mycobacterial stress-responsive regulator HspR is mediated through its hydrophobic C-terminal tail.

Authors:  Boudhayan Bandyopadhyay; Twishasri Das Gupta; Debjani Roy; Sujoy K Das Gupta
Journal:  J Bacteriol       Date:  2012-06-29       Impact factor: 3.490

Review 4.  Replication and control of circular bacterial plasmids.

Authors:  G del Solar; R Giraldo; M J Ruiz-Echevarría; M Espinosa; R Díaz-Orejas
Journal:  Microbiol Mol Biol Rev       Date:  1998-06       Impact factor: 11.056

5.  Mechanistic studies of initiator-initiator interaction and replication initiation.

Authors:  Y B Lu; H J Datta; D Bastia
Journal:  EMBO J       Date:  1998-09-01       Impact factor: 11.598

6.  Two forms of replication initiator protein: positive and negative controls.

Authors:  J Wu; M Sektas; D Chen; M Filutowicz
Journal:  Proc Natl Acad Sci U S A       Date:  1997-12-09       Impact factor: 11.205

7.  Direct physical interaction between DnaG primase and DnaB helicase of Escherichia coli is necessary for optimal synthesis of primer RNA.

Authors:  Y B Lu; P V Ratnakar; B K Mohanty; D Bastia
Journal:  Proc Natl Acad Sci U S A       Date:  1996-11-12       Impact factor: 11.205

8.  A broad host range replicon with different requirements for replication initiation in three bacterial species.

Authors:  R Caspi; M Pacek; G Consiglieri; D R Helinski; A Toukdarian; I Konieczny
Journal:  EMBO J       Date:  2001-06-15       Impact factor: 11.598

9.  A multifunctional plasmid-encoded replication initiation protein both recruits and positions an active helicase at the replication origin.

Authors:  Yong Jiang; Marcin Pacek; Donald R Helinski; Igor Konieczny; Aresa Toukdarian
Journal:  Proc Natl Acad Sci U S A       Date:  2003-06-30       Impact factor: 11.205

Review 10.  Plasmid R6K replication control.

Authors:  Sheryl A Rakowski; Marcin Filutowicz
Journal:  Plasmid       Date:  2013-03-05       Impact factor: 3.466
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