Literature DB >> 6297781

The replication initiator protein of plasmid R6K tagged with beta-galactosidase shows sequence-specific DNA-binding.

J Germino, D Bastia.   

Abstract

We have tagged the replication initiator protein of the plasmid R6K near the C-terminal end by fusion, in the correct reading frame, with the 89 amino acid long N-terminal alpha-donor polypeptide of beta-galactosidase of E. coli. This fusion was carried out with recombinant DNA methods. The protein chimera thus generated retained the activities of both initiation of DNA replication in vivo at the replication origin gamma of R6K and hydrolysis of beta-galactopyranoside when complemented in vivo with the alpha-acceptor polypeptide coded by the lac Z gene containing the M15 deletion. Using the simple and convenient assay for detecting beta-galactosidase, we have partially purified the tagged replication initiator, and have demonstrated that the protein binds to specific DNA sequences of the R6K chromosome. The protein bound to DNA sequences located at two places in the 5' untranslated leader region of the initiator protein cistron.

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Year:  1983        PMID： 6297781     DOI： 10.1016/0092-8674(83)90503-2

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


  32 in total

1.  DnaA protein is required for replication of the minimal replicon of the broad-host-range plasmid RK2 in Escherichia coli.

Authors:  P J Gaylo; N Turjman; D Bastia
Journal:  J Bacteriol       Date:  1987-10       Impact factor: 3.490

2.  Initiator protein pi can bind independently to two domains of the gamma origin core of plasmid R6K: the direct repeats and the A+T-rich segment.

Authors:  I Levchenko; M Filutowicz
Journal:  Nucleic Acids Res       Date:  1996-05-15       Impact factor: 16.971

3.  The replication initiator protein pi of the plasmid R6K specifically interacts with the host-encoded helicase DnaB.

Authors:  P V Ratnakar; B K Mohanty; M Lobert; D Bastia
Journal:  Proc Natl Acad Sci U S A       Date:  1996-05-28       Impact factor: 11.205

Review 4.  Replication and control of circular bacterial plasmids.

Authors:  G del Solar; R Giraldo; M J Ruiz-Echevarría; M Espinosa; R Díaz-Orejas
Journal:  Microbiol Mol Biol Rev       Date:  1998-06       Impact factor: 11.056

5.  Mechanistic studies of initiator-initiator interaction and replication initiation.

Authors:  Y B Lu; H J Datta; D Bastia
Journal:  EMBO J       Date:  1998-09-01       Impact factor: 11.598

6.  Two forms of replication initiator protein: positive and negative controls.

Authors:  J Wu; M Sektas; D Chen; M Filutowicz
Journal:  Proc Natl Acad Sci U S A       Date:  1997-12-09       Impact factor: 11.205

7.  Replication of the broad-host-range plasmid RK2: direct measurement of intracellular concentrations of the essential TrfA replication proteins and their effect on plasmid copy number.

Authors:  R H Durland; D R Helinski
Journal:  J Bacteriol       Date:  1990-07       Impact factor: 3.490

Review 8.  Uses of lac fusions for the study of biological problems.

Authors:  T J Silhavy; J R Beckwith
Journal:  Microbiol Rev       Date:  1985-12

9.  The repeated sequences (incB) preceding the protein E gene of plasmid mini-F are essential for replication.

Authors:  C Disqué-Kochem; U Seidel; M Helsberg; R Eichenlaub
Journal:  Mol Gen Genet       Date:  1986-01

10.  Primary structure of the essential replicon of the plasmid pSC101.

Authors:  C Vocke; D Bastia
Journal:  Proc Natl Acad Sci U S A       Date:  1983-11       Impact factor: 11.205
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