Literature DB >> 6292210

The nucleotide sequence of the replication origin beta of the plasmid R6K.

M Shon, J Germino, D Bastia.   

Abstract

We h ave identified by molecular cloning a region of 283 base pairs of the HindIII 2 fragment of R6K which corresponds to the region of the replication origin beta. This 283 base-pair DNA fragment, when present contiguously with the structural gene for the replication initiation protein of R6K, encoded in the HindIII 9-15 and part of HindIII 2 restriction fragments, will support the replication of a plasmid chimera containing the pBR322 replicon in a pol Ats host at the nonpermissive temperature. The nucleotide sequence of the region of replication origin beta has been determined. The nucleotide sequence has some homology with the ori gamma region of R6K; it has a 15-base-pair homology with the replication origin of Escherichia coli.

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Year:  1982        PMID： 6292210

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  11 in total

1.  Roles of a 106-bp origin enhancer and Escherichia coli DnaA protein in replication of plasmid R6K.

Authors:  F Wu; I Goldberg; M Filutowicz
Journal:  Nucleic Acids Res       Date:  1992-02-25       Impact factor: 16.971

2.  The replication initiator protein pi of the plasmid R6K specifically interacts with the host-encoded helicase DnaB.

Authors:  P V Ratnakar; B K Mohanty; M Lobert; D Bastia
Journal:  Proc Natl Acad Sci U S A       Date:  1996-05-28       Impact factor: 11.205

3.  Mechanistic studies of initiator-initiator interaction and replication initiation.

Authors:  Y B Lu; H J Datta; D Bastia
Journal:  EMBO J       Date:  1998-09-01       Impact factor: 11.598

4.  Binding of DnaA protein to a replication enhancer counteracts the inhibition of plasmid R6K gamma origin replication mediated by elevated levels of R6K pi protein.

Authors:  F Wu; I Levchenko; M Filutowicz
Journal:  J Bacteriol       Date:  1994-11       Impact factor: 3.490

Review 5.  Regulation of plasmid replication.

Authors:  J R Scott
Journal:  Microbiol Rev       Date:  1984-03

6.  Use of gene fusions and protein-protein interaction in the isolation of a biologically active regulatory protein: the replication initiator protein of plasmid R6K.

Authors:  J Germino; J G Gray; H Charbonneau; T Vanaman; D Bastia
Journal:  Proc Natl Acad Sci U S A       Date:  1983-11       Impact factor: 11.205

7.  Preponderance of Fis-binding sites in the R6K gamma origin and the curious effect of the penicillin resistance marker on replication of this origin in the absence of Fis.

Authors:  F Wu; J Wu; J Ehley; M Filutowicz
Journal:  J Bacteriol       Date:  1996-08       Impact factor: 3.490

8.  A DNA segment conferring stable maintenance on R6K gamma-origin core replicons.

Authors:  F Wu; I Levchenko; M Filutowicz
Journal:  J Bacteriol       Date:  1995-11       Impact factor: 3.490

9.  Altered (copy-up) forms of initiator protein pi suppress the point mutations inactivating the gamma origin of plasmid R6K.

Authors:  M Urh; Y Flashner; A Shafferman; M Filutowicz
Journal:  J Bacteriol       Date:  1995-12       Impact factor: 3.490

10.  Replication of the R6K plasmid during the Escherichia coli cell cycle.

Authors:  J D Keasling; B O Palsson; S Cooper
Journal:  J Bacteriol       Date:  1992-02       Impact factor: 3.490
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