Literature DB >> 8586678

Single-step PCR for detection of Brucella spp. from blood and milk of infected animals.

D S Leal-Klevezas1, I O Martínez-Vázquez, A López-Merino, J P Martínez-Soriano.   

Abstract

A versatile method for the extraction of Brucella DNA and PCR are presented as reliable tools for the detection of Brucella spp. from body fluids of infected animals. Two oligonucleotides homologous to regions of the gene encoding for an outer membrane protein (omp-2) were designed to detect the pathogen from milk and/or blood of infected goats, bovines, and human patients. The sensitivity of our test and its ability to detect the pathogen in samples from the field reveal a promising advance in the diagnosis of brucellosis in animals and humans.

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Year:  1995        PMID： 8586678      PMCID： PMC228649          DOI： 10.1128/jcm.33.12.3087-3090.1995

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  19 in total

1.  Basic local alignment search tool.

Authors:  S F Altschul; W Gish; W Miller; E W Myers; D J Lipman
Journal:  J Mol Biol       Date:  1990-10-05       Impact factor: 5.469

2.  Detection of Brucella by polymerase chain reaction in bovine fetal and maternal tissues.

Authors:  A Fekete; J A Bantle; S M Halling
Journal:  J Vet Diagn Invest       Date:  1992-01       Impact factor: 1.279

3.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors:  R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

Review 4.  The epidemiology of bovine brucellosis.

Authors:  P Nicoletti
Journal:  Adv Vet Sci Comp Med       Date:  1980

5.  Serologic and bacteriologic test results after adult vaccination with strain 19 in three dairy herds infected with brucellosis.

Authors:  R E Breitmeyer; D W Hird; T E Carpenter
Journal:  J Am Vet Med Assoc       Date:  1992-03-15       Impact factor: 1.936

6.  DNA sequence and expression of the 36-kilodalton outer membrane protein gene of Brucella abortus.

Authors:  T A Ficht; S W Bearden; B A Sowa; L G Adams
Journal:  Infect Immun       Date:  1989-11       Impact factor: 3.441

7.  Antigenic relationships of the lipopolysaccharides of Escherichia hermannii strains with those of Escherichia coli O157:H7, Brucella melitensis, and Brucella abortus.

Authors:  M B Perry; D R Bundle
Journal:  Infect Immun       Date:  1990-05       Impact factor: 3.441

8.  Preliminary development of a diagnostic test for Brucella using polymerase chain reaction.

Authors:  A Fekete; J A Bantle; S M Halling; M R Sanborn
Journal:  J Appl Bacteriol       Date:  1990-08

9.  Identification of Brucella spp. by using the polymerase chain reaction.

Authors:  L Herman; H De Ridder
Journal:  Appl Environ Microbiol       Date:  1992-06       Impact factor: 4.792

10.  Genetic variation at the omp2 porin locus of the brucellae: species-specific markers.

Authors:  T A Ficht; S W Bearden; B A Sowa; H Marquis
Journal:  Mol Microbiol       Date:  1990-07       Impact factor: 3.501
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  42 in total

1.  Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR.

Authors:  C Romero; I Lopez-Goñi
Journal:  Appl Environ Microbiol       Date:  1999-08       Impact factor: 4.792

2.  Antifreeze solution improves DNA recovery by preserving the integrity of pathogen-infected blood and other tissues.

Authors:  D S Leal-Klevezas; I O Martínez-Vázquez; B Cuevas-Hernández; J P Martínez-Soriano
Journal:  Clin Diagn Lab Immunol       Date:  2000-11

3.  Use of an immunobinding test on nitrocellulose paper to diagnose caprine brucellosis.

Authors:  E Santellano-Estrada; F Infante; E Díaz-Aparicio; G H Flores-Gutiérrez
Journal:  Vet Res Commun       Date:  2004-01       Impact factor: 2.459

4.  A multiplex approach to molecular detection of Brucella abortus and/or Mycobacterium bovis infection in cattle.

Authors:  S Sreevatsan; J B Bookout; F Ringpis; V S Perumaalla; T A Ficht; L G Adams; S D Hagius; P H Elzer; B J Bricker; G K Kumar; M Rajasekhar; S Isloor; R R Barathur
Journal:  J Clin Microbiol       Date:  2000-07       Impact factor: 5.948

5.  Comparison of conventional and non-conventional techniques for the diagnosis of bovine brucellosis in Sudan.

Authors:  Amel Abdalla; Mohamed E Hamid
Journal:  Trop Anim Health Prod       Date:  2011-12-27       Impact factor: 1.559

6.  Limited genetic diversity of Brucella spp.

Authors:  B Gándara; A L Merino; M A Rogel; E Martínez-Romero
Journal:  J Clin Microbiol       Date:  2001-01       Impact factor: 5.948

Review 7.  Laboratory Diagnosis of Human Brucellosis.

Authors:  Pablo Yagupsky; Pilar Morata; Juan D Colmenero
Journal:  Clin Microbiol Rev       Date:  2019-11-13       Impact factor: 26.132

8.  Comparison of commercial DNA preparation kits for the detection of Brucellae in tissue using quantitative real-time PCR.

Authors:  Herbert Tomaso; Mireille Kattar; Meike Eickhoff; Ulrich Wernery; Sascha Al Dahouk; Eberhard Straube; Heinrich Neubauer; Holger C Scholz
Journal:  BMC Infect Dis       Date:  2010-04-20       Impact factor: 3.090

9.  Comparison of diagnostic tests for the detection of bovine brucellosis in the natural cases of abortion.

Authors:  V Mahajan; H S Banga; G Filia; M P Gupta; K Gupta
Journal:  Iran J Vet Res       Date:  2017       Impact factor: 1.376

10.  IS711-based real-time PCR assay as a tool for detection of Brucella spp. in wild boars and comparison with bacterial isolation and serology.

Authors:  Vladimira Hinić; Isabelle Brodard; Andreas Thomann; Milena Holub; Raymond Miserez; Carlos Abril
Journal:  BMC Vet Res       Date:  2009-07-14       Impact factor: 2.741
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