Literature DB >> 10427076

Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR.

C Romero1, I Lopez-Goñi.   

Abstract

Different methods of extraction of bacterial DNA from bovine milk to improve the direct detection of Brucella by PCR were evaluated. We found that the use of a lysis buffer with high concentrations of Tris, EDTA, and NaCl, high concentrations of sodium dodecyl sulfate and proteinase K, and high temperatures of incubation was necessary for the efficient extraction of Brucella DNA. The limit of detection by PCR was 5 to 50 Brucella CFU/ml of milk.

Entities:  

Mesh：

Substances：

Year:  1999        PMID： 10427076      PMCID： PMC91561     

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  8 in total

Review 1.  Properties of the outer membrane of Brucella.

Authors:  I Moriyón; C Gamazo; R Díaz
Journal:  Ann Inst Pasteur Microbiol       Date:  1987 Jan-Feb

2.  Alkaline decontamination of sputum specimens adversely affects stability of mycobacterial mRNA.

Authors:  L E Desjardin; M D Perkins; L Teixeira; M D Cave; K D Eisenach
Journal:  J Clin Microbiol       Date:  1996-10       Impact factor: 5.948

3.  Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle.

Authors:  C Romero; M Pardo; M J Grillo; R Diaz; J M Blasco; I Lopez-Goñi
Journal:  J Clin Microbiol       Date:  1995-12       Impact factor: 5.948

4.  Improved amplification of microbial DNA from blood cultures by removal of the PCR inhibitor sodium polyanetholesulfonate.

Authors:  D N Fredricks; D A Relman
Journal:  J Clin Microbiol       Date:  1998-10       Impact factor: 5.948

5.  Single-step PCR for detection of Brucella spp. from blood and milk of infected animals.

Authors:  D S Leal-Klevezas; I O Martínez-Vázquez; A López-Merino; J P Martínez-Soriano
Journal:  J Clin Microbiol       Date:  1995-12       Impact factor: 5.948

6.  Effects of nonionic, ionic, and dipolar ionic detergents and EDTA on the Brucella cell envelope.

Authors:  I Moriyon; D T Berman
Journal:  J Bacteriol       Date:  1982-11       Impact factor: 3.490

7.  Specific detection of Brucella DNA by PCR.

Authors:  C Romero; C Gamazo; M Pardo; I López-Goñi
Journal:  J Clin Microbiol       Date:  1995-03       Impact factor: 5.948

8.  Direct detection of Brucella spp. in raw milk by PCR and reverse hybridization with 16S-23S rRNA spacer probes.

Authors:  N P Rijpens; G Jannes; M Van Asbroeck; R Rossau; L M Herman
Journal:  Appl Environ Microbiol       Date:  1996-05       Impact factor: 4.792
  8 in total
  14 in total

1.  Evaluation of DNA extraction methods for use in combination with SYBR green I real-time PCR to detect Salmonella enterica serotype enteritidis in poultry.

Authors:  Dario De Medici; Luciana Croci; Elisabetta Delibato; Simona Di Pasquale; Emma Filetici; Laura Toti
Journal:  Appl Environ Microbiol       Date:  2003-06       Impact factor: 4.792

2.  Identification and tracing of Bifidobacterium species by use of enterobacterial repetitive intergenic consensus sequences.

Authors:  Marco Ventura; Valerie Meylan; Ralf Zink
Journal:  Appl Environ Microbiol       Date:  2003-07       Impact factor: 4.792

3.  Diagnostic real-time PCR assays for the detection of emetic Bacillus cereus strains in foods and recent food-borne outbreaks.

Authors:  Martina Fricker; Ute Messelhäusser; Ulrich Busch; Siegfried Scherer; Monika Ehling-Schulz
Journal:  Appl Environ Microbiol       Date:  2007-01-26       Impact factor: 4.792

4.  Validation of real-time PCR technique for detection of Mycobacterium bovis and Brucella abortus in bovine raw milk.

Authors:  Débora R Mascarenhas; David Germano G Schwarz; Antônio A Fonseca Júnior; Tatiana F P Oliveira; Maria A S Moreira
Journal:  Braz J Microbiol       Date:  2020-06-22       Impact factor: 2.476

5.  Food safety in raw milk production: risk factors associated to bacterial DNA contamination.

Authors:  Cristine Cerva; Carolina Bremm; Emily Marques dos Reis; André Vinícius Andrade Bezerra; Márcia Regina Loiko; Cláudio Estêvão Farias da Cruz; Alexander Cenci; Fabiana Quoos Mayer
Journal:  Trop Anim Health Prod       Date:  2014-04-09       Impact factor: 1.559

Review 6.  Review of detection of Brucella spp. by polymerase chain reaction.

Authors:  Wei Ling Yu; Klaus Nielsen
Journal:  Croat Med J       Date:  2010-08       Impact factor: 1.351

7.  Real-time PCR detection of Brucella abortus: a comparative study of SYBR green I, 5'-exonuclease, and hybridization probe assays.

Authors:  D T Newby; T L Hadfield; F F Roberto
Journal:  Appl Environ Microbiol       Date:  2003-08       Impact factor: 4.792

8.  Development of a bead-based Luminex assay using lipopolysaccharide specific monoclonal antibodies to detect biological threats from Brucella species.

Authors:  Angelika Silbereisen; Marco Tamborrini; Matthias Wittwer; Nadia Schürch; Gerd Pluschke
Journal:  BMC Microbiol       Date:  2015-10-05       Impact factor: 3.605

9.  Contamination of Bovine, Sheep and Goat Meat with Brucella Spp.

Authors:  Francesco Casalinuovo; Lucia Ciambrone; Antonio Cacia; Paola Rippa
Journal:  Ital J Food Saf       Date:  2016-06-03

10.  Application and evaluation of the MLVA typing assay for the Brucella abortus strains isolated in Korea.

Authors:  Moon Her; Sung-Il Kang; Dong-Hee Cho; Yun-Sang Cho; In-Yeong Hwang; Young-Ran Heo; Suk-Chan Jung; Han-Sang Yoo
Journal:  BMC Microbiol       Date:  2009-10-29       Impact factor: 3.605
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.