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Literature DB >> 1377903

Identification of Brucella spp. by using the polymerase chain reaction.

Abstract

The application of two synthetic oligonucleotides as probes and as primers in the polymerase chain reaction is presented for a specific, sensitive, and quick identification of Brucella spp. The specific oligonucleotide sequences were chosen on the basis of a 16S rRNA sequence alignment between Brucella abortus and Agrobacterium tumefaciens.

Entities: Chemical Species

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Year: 1992 PMID： 1377903 PMCID： PMC195734 DOI： 10.1128/aem.58.6.2099-2101.1992

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

4 in total

1. Nucleotide sequence of the 16S rRNA from Brucella abortus.

Authors: M Dorsch; E Moreno; E Stackebrandt
Journal: Nucleic Acids Res Date: 1989-02-25 Impact factor: 16.971

2. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors: R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal: Science Date: 1988-01-29 Impact factor: 47.728

3. Preliminary development of a diagnostic test for Brucella using polymerase chain reaction.

Authors: A Fekete; J A Bantle; S M Halling; M R Sanborn
Journal: J Appl Bacteriol Date: 1990-08

4. Introduction of pAM beta 1 into Listeria monocytogenes by conjugation and homology between native L. monocytogenes plasmids.

Authors: R K Flamm; D J Hinrichs; M F Thomashow
Journal: Infect Immun Date: 1984-04 Impact factor: 3.441

4 in total

26 in total

1. 16S rRNA-targeted polymerase chain reaction and oligonucleotide hybridization to screen for Azoarcus spp., grass-associated diazotrophs.

Authors: T Hurek; S Burggraf; C R Woese; B Reinhold-Hurek
Journal: Appl Environ Microbiol Date: 1993-11 Impact factor: 4.792

2. Fluorescent whole-cell hybridization with 16S rRNA-targeted oligonucleotide probes to identify Brucella spp. by flow cytometry.

Authors: L Fernández-Lago; F J Vallejo; I Trujillano; N Vizcaíno
Journal: J Clin Microbiol Date: 2000-07 Impact factor: 5.948

3. Advanced multiplex PCR assay for differentiation of Brucella species.

Authors: Sung-Il Kang; Moon Her; Jong Wan Kim; Ji-Yeon Kim; Kyung Yuk Ko; Yun-Mi Ha; Suk Chan Jung
Journal: Appl Environ Microbiol Date: 2011-06-10 Impact factor: 4.792

4. Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle.

Authors: C Romero; M Pardo; M J Grillo; R Diaz; J M Blasco; I Lopez-Goñi
Journal: J Clin Microbiol Date: 1995-12 Impact factor: 5.948

Review 5. Laboratory Diagnosis of Human Brucellosis.

Authors: Pablo Yagupsky; Pilar Morata; Juan D Colmenero
Journal: Clin Microbiol Rev Date: 2019-11-13 Impact factor: 26.132

6. Evaluation of PCR methods for detection of Brucella strains from culture and tissues.

Authors: Alper Çiftci; Tuba İça; Serap Savaşan; Barış Sareyyüpoğlu; Mehmet Akan; Kadir Serdar Diker
Journal: Trop Anim Health Prod Date: 2017-03-03 Impact factor: 1.559

7. Single-step PCR for detection of Brucella spp. from blood and milk of infected animals.

Authors: D S Leal-Klevezas; I O Martínez-Vázquez; A López-Merino; J P Martínez-Soriano
Journal: J Clin Microbiol Date: 1995-12 Impact factor: 5.948

8. Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR.

Authors: B J Bricker; S M Halling
Journal: J Clin Microbiol Date: 1994-11 Impact factor: 5.948

9. IS711-based real-time PCR assay as a tool for detection of Brucella spp. in wild boars and comparison with bacterial isolation and serology.

Authors: Vladimira Hinić; Isabelle Brodard; Andreas Thomann; Milena Holub; Raymond Miserez; Carlos Abril
Journal: BMC Vet Res Date: 2009-07-14 Impact factor: 2.741

10. Application and evaluation of the MLVA typing assay for the Brucella abortus strains isolated in Korea.

Authors: Moon Her; Sung-Il Kang; Dong-Hee Cho; Yun-Sang Cho; In-Yeong Hwang; Young-Ran Heo; Suk-Chan Jung; Han-Sang Yoo
Journal: BMC Microbiol Date: 2009-10-29 Impact factor: 3.605