Literature DB >> 8557255

Direct detection of disomy in human sperm by the PRINS technique.

F Pellestor1, I Quennesson, L Coignet, A Girardet, B Andréo, J P Charlieu.   

Abstract

We report the use of the PRimedIN Situ (PRINS) labelling technique for the direct estimation of disomy rates for various chromosomes in human sperm. The PRINS technique provides a rapid and reliable method for chromosome screening since specific labelling may be obtained in less than 2 h. In the present study, the disomy rates of chromosomes 2, 5, 9, 12 and 18 were investigated. The incidences of disomy are similar for all these chromosomes, ranging from 0.27% to 0.33%. These findings suggest an equal distribution of aneuploidies among autosomes in human sperm.

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Year:  1996        PMID： 8557255     DOI： 10.1007/bf00218827

Source DB:  PubMed          Journal:  Hum Genet        ISSN： 0340-6717            Impact factor:   4.132


  21 in total

1.  Prenatal diagnosis with repetitive in situ hybridization probes.

Authors:  R V Lebo; R R Flandermeyer; R Diukman; E D Lynch; J A Lepercq; M S Golbus
Journal:  Am J Med Genet       Date:  1992-07-15

2.  Distribution of aneuploidy in human gametes: comparison between human sperm and oocytes.

Authors:  R H Martin; E Ko; A Rademaker
Journal:  Am J Med Genet       Date:  1991-06-01

3.  Non-isotopic detection of chromosome 1 in human meiosis and demonstration of disomic sperm nuclei.

Authors:  M Guttenbach; M Schmid
Journal:  Hum Genet       Date:  1991-07       Impact factor: 4.132

Review 4.  PCR amplification of specific alleles: rapid detection of known mutations and polymorphisms.

Authors:  C D Bottema; S S Sommer
Journal:  Mutat Res       Date:  1993-07       Impact factor: 2.433

5.  Simultaneous detection of X- and Y-bearing human sperm by double fluorescence in situ hybridization.

Authors:  T L Han; J H Ford; G C Webb; S P Flaherty; A Correll; C D Matthews
Journal:  Mol Reprod Dev       Date:  1993-03       Impact factor: 2.609

6.  Use of the primed in situ labelling (PRINS) technique for a rapid detection of chromosomes 13, 16, 18, 21, X and Y.

Authors:  F Pellestor; A Girardet; G Lefort; B Andréo; J P Charlieu
Journal:  Hum Genet       Date:  1995-01       Impact factor: 4.132

7.  A polymorphic alpha satellite sequence specific for human chromosome 13 detected by oligonucleotide primed in situ labelling (PRINS).

Authors:  F Pellestor; A Girardet; B Andréo; J P Charlieu
Journal:  Hum Genet       Date:  1994-10       Impact factor: 4.132

8.  Fast, sensitive multicolor detection of nucleic acids in situ by PRimed IN Situ labeling (PRINS).

Authors:  J Hindkjaer; J Koch; C Terkelsen; C A Brandt; S Kølvraa; L Bolund
Journal:  Cytogenet Cell Genet       Date:  1994

9.  Differential distribution of aneuploidy in human gametes according to their sex.

Authors:  F Pellestor
Journal:  Hum Reprod       Date:  1991-10       Impact factor: 6.918

10.  Nonisotopic in situ hybridization as a method for nondisjunction studies in human spermatozoa.

Authors:  E Coonen; M H Pieters; J C Dumoulin; H Meyer; J L Evers; F C Ramaekers; J P Geraedts
Journal:  Mol Reprod Dev       Date:  1991-01       Impact factor: 2.609
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  3 in total

1.  Fluorescence in situ hybridization with chromosome paint probes: a novel approach to assess aneuploidy in human sperm nuclei.

Authors:  N Rives; S Wust; B David; V Duchesne; G Joly; B Mace
Journal:  J Assist Reprod Genet       Date:  1999-01       Impact factor: 3.412

2.  Using PRINS for gene mapping in polytene chromosomes.

Authors:  H F Gu; M I Lind; L Wieslander; U Landegren; K Söderhäll; O Melefors
Journal:  Chromosome Res       Date:  1997-11       Impact factor: 5.239

3.  PRINS and C-PRINS: promising tools for the physical mapping of the lupin genome.

Authors:  Anna Kaczmarek; Barbara Naganowska; Bogdan Wolko
Journal:  Cell Mol Biol Lett       Date:  2006-11-13       Impact factor: 5.787
  3 in total

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