Literature DB >> 7814017

Use of the primed in situ labelling (PRINS) technique for a rapid detection of chromosomes 13, 16, 18, 21, X and Y.

F Pellestor1, A Girardet, G Lefort, B Andréo, J P Charlieu.   

Abstract

The primed in situ labelling (PRINS) technique is an alternative to in situ hybridization for chromosomal screening. We have developed a semi-automatic PRINS protocol, using a programmable thermocycler. The method has been successfully tested with specific primers for chromosomes, 13, 16, 18, 21, X and Y. Specific chromosome detection has been obtained on both metaphases and interphase nuclei. This suggests that PRINS may be a reliable technique for detecting aneuploidies and some chromosomal aberrations.

Mesh：

Substances：

Year:  1995        PMID： 7814017     DOI： 10.1007/bf00225066

Source DB:  PubMed          Journal:  Hum Genet        ISSN： 0340-6717            Impact factor:   4.132


  14 in total

1.  In situ hybridization studies for the detection of common aneuploidies in CVS.

Authors:  L Zahed; M Murer-Orlando; M Vekemans
Journal:  Prenat Diagn       Date:  1992-06       Impact factor: 3.050

2.  An efficient method for selecting unique-sequence clones from DNA libraries and its application to fluorescent staining of human chromosome 21 using in situ hybridization.

Authors:  J C Fuscoe; C C Collins; D Pinkel; J W Gray
Journal:  Genomics       Date:  1989-07       Impact factor: 5.736

3.  Rapid detection of chromosome aneuploidies in uncultured amniocytes by using fluorescence in situ hybridization (FISH).

Authors:  K Klinger; G Landes; D Shook; R Harvey; L Lopez; P Locke; T Lerner; R Osathanondh; B Leverone; T Houseal
Journal:  Am J Hum Genet       Date:  1992-07       Impact factor: 11.025

4.  Prenatal diagnosis with repetitive in situ hybridization probes.

Authors:  R V Lebo; R R Flandermeyer; R Diukman; E D Lynch; J A Lepercq; M S Golbus
Journal:  Am J Med Genet       Date:  1992-07-15

5.  An improved method for chromosome-specific labeling of alpha satellite DNA in situ by using denatured double-stranded DNA probes as primers in a primed in situ labeling (PRINS) procedure.

Authors:  J Koch; J Hindkjaer; J Mogensen; S Kølvraa; L Bolund
Journal:  Genet Anal Tech Appl       Date:  1991-09

Review 6.  Gene mapping by in situ hybridization.

Authors:  B J Trask
Journal:  Curr Opin Genet Dev       Date:  1991-06       Impact factor: 5.578

7.  Multicolor fluorescence in situ hybridization for the simultaneous detection of probe sets for chromosomes 13, 18, 21, X and Y in uncultured amniotic fluid cells.

Authors:  T Ried; G Landes; W Dackowski; K Klinger; D C Ward
Journal:  Hum Mol Genet       Date:  1992-08       Impact factor: 6.150

8.  Detection of chromosome aberrations in metaphase and interphase tumor cells by in situ hybridization using chromosome-specific library probes.

Authors:  T Cremer; P Lichter; J Borden; D C Ward; L Manuelidis
Journal:  Hum Genet       Date:  1988-11       Impact factor: 4.132

9.  Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4.

Authors:  D Pinkel; J Landegent; C Collins; J Fuscoe; R Segraves; J Lucas; J Gray
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205

10.  Oligonucleotide-primed in situ DNA synthesis (PRINS): a method for chromosome mapping, banding, and investigation of sequence organization.

Authors:  J Gosden; D Hanratty; J Starling; J Fantes; A Mitchell; D Porteous
Journal:  Cytogenet Cell Genet       Date:  1991
View more
  6 in total

1.  The high incidence of STR D21S1446 homozygosity in Han and She populations living in South Eastern China.

Authors:  Jin-Fang Wu; Lu Xia; Yu-Xin Liu; Xin Zhang; Chen Li; Ming-Li Li
Journal:  J Assist Reprod Genet       Date:  2011-12-23       Impact factor: 3.412

2.  Rapid chromosome detection in human gametes, zygotes, and preimplantation embryos using the PRINS technique.

Authors:  F Pellestor; A Girardet; G Lefort; B Andréo; J P Charlieu
Journal:  J Assist Reprod Genet       Date:  1996-09       Impact factor: 3.412

3.  Rapid detection of euchromatin by Alu-PRINS: use in clinical cytogenetics.

Authors:  D F Callen; M Y Yip; H J Eyre
Journal:  Chromosome Res       Date:  1997-04       Impact factor: 5.239

4.  Primed in situ (PRINS) labelling with Alu and satellite primers for rapid characterization of human chromosomes in hybrid cell lines.

Authors:  P Coullin; B Andréo; J P Charlieu; J J Candelier; F Pellestor
Journal:  Chromosome Res       Date:  1997-08       Impact factor: 5.239

5.  Direct detection of disomy in human sperm by the PRINS technique.

Authors:  F Pellestor; I Quennesson; L Coignet; A Girardet; B Andréo; J P Charlieu
Journal:  Hum Genet       Date:  1996-01       Impact factor: 4.132

Review 6.  Classical, Molecular, and Genomic Cytogenetics of the Pig, a Clinical Perspective.

Authors:  Brendan Donaldson; Daniel A F Villagomez; W Allan King
Journal:  Animals (Basel)       Date:  2021-04-27       Impact factor: 2.752
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.