Literature DB >> 1994976

Nonisotopic in situ hybridization as a method for nondisjunction studies in human spermatozoa.

E Coonen1, M H Pieters, J C Dumoulin, H Meyer, J L Evers, F C Ramaekers, J P Geraedts.   

Abstract

Human spermatozoa were studied with a nonradioactive in situ hybridization method. Using a chemically modified DNA probe and immunocytochemical reactions for visualization, it was possible to obtain hybridization signals in 31 of 32 semen samples. Positive hybridization reactions, depending on cell accessibility, varied from 40% to over 90% for the different samples. Using a chromosome 1-specific DNA probe, disomy for this chromosome was found in 0.67% of all accessible sperm cells.

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Year:  1991        PMID： 1994976     DOI： 10.1002/mrd.1080280104

Source DB:  PubMed          Journal:  Mol Reprod Dev        ISSN： 1040-452X            Impact factor:   2.609


  14 in total

1.  Fluorescence in situ hybridization with chromosome paint probes: a novel approach to assess aneuploidy in human sperm nuclei.

Authors:  N Rives; S Wust; B David; V Duchesne; G Joly; B Mace
Journal:  J Assist Reprod Genet       Date:  1999-01       Impact factor: 3.412

2.  Genetics of human sperm.

Authors:  R H Martin
Journal:  J Assist Reprod Genet       Date:  1998-05       Impact factor: 3.412

3.  Well-defined genome architecture in the human sperm nucleus.

Authors:  A O Zalensky; M J Allen; A Kobayashi; I A Zalenskaya; R Balhórn; E M Bradbury
Journal:  Chromosoma       Date:  1995-05       Impact factor: 4.316

4.  Organization of centromeres in the decondensed nuclei of mature human sperm.

Authors:  A O Zalensky; J W Breneman; I A Zalenskaya; B R Brinkley; E M Bradbury
Journal:  Chromosoma       Date:  1993-09       Impact factor: 4.316

5.  Incidence of chromosome 18 disomy in human sperm nuclei as detected by nonisotopic in situ hybridization.

Authors:  M Guttenbach; R Schakowski; M Schmid
Journal:  Hum Genet       Date:  1994-04       Impact factor: 4.132

6.  Direct detection of disomy in human sperm by the PRINS technique.

Authors:  F Pellestor; I Quennesson; L Coignet; A Girardet; B Andréo; J P Charlieu
Journal:  Hum Genet       Date:  1996-01       Impact factor: 4.132

7.  Incidence of chromosome 3, 7, 10, 11, 17 and X disomy in mature human sperm nuclei as determined by nonradioactive in situ hybridization.

Authors:  M Guttenbach; R Schakowski; M Schmid
Journal:  Hum Genet       Date:  1994-01       Impact factor: 4.132

8.  Numerical chromosome abnormalities in spermatozoa of fertile and infertile men detected by fluorescence in situ hybridization.

Authors:  N Miharu; R G Best; S R Young
Journal:  Hum Genet       Date:  1994-05       Impact factor: 4.132

9.  Detection of aneuploid human sperm by fluorescence in situ hybridization: evidence for a donor difference in frequency of sperm disomic for chromosomes 1 and Y.

Authors:  W A Robbins; R Segraves; D Pinkel; A J Wyrobek
Journal:  Am J Hum Genet       Date:  1993-04       Impact factor: 11.025

10.  Aneuploidy detection in human sperm nuclei using fluorescence in situ hybridization.

Authors:  J M Holmes; R H Martin
Journal:  Hum Genet       Date:  1993-03       Impact factor: 4.132
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