Literature DB >> 8524832

Mapping by multifragment cloning in vivo.

D L Marykwas1, S E Passmore.   

Abstract

An efficient method for mapping mutations is described in which hybrid genes, derived partly from mutant and partly from wild-type DNA, are obtained in vivo by homologous recombination of multiple fragments. The recombinants are formed in a strain in which their phenotypes are immediately apparent. This method was developed to identify changes that disrupt protein-protein interactions demonstrable by the two-hybrid system in yeast. However, it can be extended to any system where recombination is possible, provided an assay is available to distinguish between mutant and wild-type phenotypes.

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Year:  1995        PMID： 8524832      PMCID： PMC40470          DOI： 10.1073/pnas.92.25.11701

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  19 in total

1.  A rapid method for localized mutagenesis of yeast genes.

Authors:  D Muhlrad; R Hunter; R Parker
Journal:  Yeast       Date:  1992-02       Impact factor: 3.239

2.  The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.

Authors:  C T Chien; P L Bartel; R Sternglanz; S Fields
Journal:  Proc Natl Acad Sci U S A       Date:  1991-11-01       Impact factor: 11.205

3.  Mutants of GAL4 protein altered in an activation function.

Authors:  G Gill; M Ptashne
Journal:  Cell       Date:  1987-10-09       Impact factor: 41.582

4.  High efficiency transformation of E. coli by high voltage electroporation.

Authors:  W J Dower; J F Miller; C W Ragsdale
Journal:  Nucleic Acids Res       Date:  1988-07-11       Impact factor: 16.971

5.  Fine structure recombinational analysis of cloned genes using yeast transformation.

Authors:  S Kunes; H Ma; K Overbye; M S Fox; D Botstein
Journal:  Genetics       Date:  1987-01       Impact factor: 4.562

6.  A new class of yeast transcriptional activators.

Authors:  J Ma; M Ptashne
Journal:  Cell       Date:  1987-10-09       Impact factor: 41.582

7.  Rapid and efficient site-specific mutagenesis without phenotypic selection.

Authors:  T A Kunkel
Journal:  Proc Natl Acad Sci U S A       Date:  1985-01       Impact factor: 11.205

Review 8.  Directed mutagenesis.

Authors:  D Shortle; D DiMaio; D Nathans
Journal:  Annu Rev Genet       Date:  1981       Impact factor: 16.830

9.  Oligonucleotide-directed mutagenesis: a simple method using two oligonucleotide primers and a single-stranded DNA template.

Authors:  M J Zoller; M Smith
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

10.  In vivo cloning of PCR products in E. coli.

Authors:  J D Oliner; K W Kinzler; B Vogelstein
Journal:  Nucleic Acids Res       Date:  1993-11-11       Impact factor: 16.971
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  9 in total

1.  One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome.

Authors:  Daniel G Gibson; Gwynedd A Benders; Kevin C Axelrod; Jayshree Zaveri; Mikkel A Algire; Monzia Moodie; Michael G Montague; J Craig Venter; Hamilton O Smith; Clyde A Hutchison
Journal:  Proc Natl Acad Sci U S A       Date:  2008-12-10       Impact factor: 11.205

2.  Structural characteristic of folding/unfolding intermediate of pokeweed anti-viral protein revealed by time-resolved fluorescence.

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3.  Yeast-based recombineering of DNA fragments into plant transformation vectors by one-step transformation.

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Journal:  Plant Cell Rep       Date:  2007-08-07       Impact factor: 4.570

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Authors:  D L Marykwas; H C Berg
Journal:  J Bacteriol       Date:  1996-03       Impact factor: 3.490

5.  Shuttle vectors for facile gap repair cloning and integration into a neutral locus in Candida albicans.

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Journal:  Microbiology       Date:  2013-01-10       Impact factor: 2.777

6.  Sugar binding effects on the enzymatic reaction and conformation near the active site of pokeweed antiviral protein revealed by fluorescence spectroscopy.

Authors:  Hiromichi Nakashima; Yukihiro Fukunaga; Ryosuke Ueno; Etsuko Nishimoto
Journal:  J Fluoresc       Date:  2014-04-04       Impact factor: 2.217

7.  Efficient library construction by in vivo recombination with a telomere-originated autonomously replicating sequence of Hansenula polymorpha.

Authors:  So-Young Kim; Jung-Hoon Sohn; Jung-Hoon Bae; Yu-Ryang Pyun; Michael O Agaphonov; Michael D Ter-Avanesyan; Eui-Sung Choi
Journal:  Appl Environ Microbiol       Date:  2003-08       Impact factor: 4.792

8.  Strategies to facilitate the development of uncloned or cloned infectious full-length viral cDNAs: Apple chlorotic leaf spot virus as a case study.

Authors:  Fater Youssef; Armelle Marais; Chantal Faure; Pascal Gentit; Thierry Candresse
Journal:  Virol J       Date:  2011-10-31       Impact factor: 4.099

9.  Ultra-low background DNA cloning system.

Authors:  Kenta Goto; Yukio Nagano
Journal:  PLoS One       Date:  2013-02-08       Impact factor: 3.240
  9 in total

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