Literature DB >> 1561838

A rapid method for localized mutagenesis of yeast genes.

D Muhlrad1, R Hunter, R Parker.   

Abstract

We have developed a simple procedure for the localized mutagenesis of yeast genes. In this technique the region of interest is first amplified under mutagenic polymerase chain reaction (PCR) conditions. Cotransformation of the PCR product with a gapped plasmid containing homology to both ends of the PCR product allows in vivo recombination to repair the gap with the mutagenized DNA. This procedure is efficient, allows targeting of specific regions for mutagenesis, and requires no subcloning steps in Escherichia coli.

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Year:  1992        PMID： 1561838     DOI： 10.1002/yea.320080202

Source DB:  PubMed          Journal:  Yeast        ISSN： 0749-503X            Impact factor:   3.239


  245 in total

1.  A genetic strategy to eliminate self-activator baits prior to high-throughput yeast two-hybrid screens.

Authors:  A J Walhout; M Vidal
Journal:  Genome Res       Date:  1999-11       Impact factor: 9.043

2.  Activation mutants in yeast RNA polymerase II subunit RPB3 provide evidence for a structurally conserved surface required for activation in eukaryotes and bacteria.

Authors:  Q Tan; K L Linask; R H Ebright; N A Woychik
Journal:  Genes Dev       Date:  2000-02-01       Impact factor: 11.361

3.  Specific retrieval of the exocytic SNARE Snc1p from early yeast endosomes.

Authors:  M J Lewis; B J Nichols; C Prescianotto-Baschong; H Riezman; H R Pelham
Journal:  Mol Biol Cell       Date:  2000-01       Impact factor: 4.138

4.  Mutations that affect dimer formation and helicase activity of the hepatitis C virus helicase.

Authors:  Y L Khu; E Koh; S P Lim; Y H Tan; S Brenner; S G Lim; W J Hong; P Y Goh
Journal:  J Virol       Date:  2001-01       Impact factor: 5.103

5.  An activation-specific role for transcription factor TFIIB in vivo.

Authors:  W H Wu; M Hampsey
Journal:  Proc Natl Acad Sci U S A       Date:  1999-03-16       Impact factor: 11.205

6.  Genome-scale cloning and expression of individual open reading frames using topoisomerase I-mediated ligation.

Authors:  J A Heyman; J Cornthwaite; L Foncerrada; J R Gilmore; E Gontang; K J Hartman; C L Hernandez; R Hood; H M Hull; W Y Lee; R Marcil; E J Marsh; K M Mudd; M J Patino; T J Purcell; J J Rowland; M L Sindici; J P Hoeffler
Journal:  Genome Res       Date:  1999-04       Impact factor: 9.043

7.  The recruitment of RNA polymerase I on rDNA is mediated by the interaction of the A43 subunit with Rrn3.

Authors:  G Peyroche; P Milkereit; N Bischler; H Tschochner; P Schultz; A Sentenac; C Carles; M Riva
Journal:  EMBO J       Date:  2000-10-16       Impact factor: 11.598

8.  Control of microtubule dynamics by Stu2p is essential for spindle orientation and metaphase chromosome alignment in yeast.

Authors:  K A Kosco; C G Pearson; P S Maddox; P J Wang; I R Adams; E D Salmon; K Bloom; T C Huffaker
Journal:  Mol Biol Cell       Date:  2001-09       Impact factor: 4.138

9.  Identification of a functional domain within the essential core of histone H3 that is required for telomeric and HM silencing in Saccharomyces cerevisiae.

Authors:  Jeffrey S Thompson; Marilyn L Snow; Summer Giles; Leslie E McPherson; Michael Grunstein
Journal:  Genetics       Date:  2003-01       Impact factor: 4.562

10.  Clathrin light and heavy chain interface: alpha-helix binding superhelix loops via critical tryptophans.

Authors:  Chih-Ying Chen; Michael L Reese; Peter K Hwang; Nobuyuki Ota; David Agard; Frances M Brodsky
Journal:  EMBO J       Date:  2002-11-15       Impact factor: 11.598
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