| Literature DB >> 19073939 |
Daniel G Gibson1, Gwynedd A Benders, Kevin C Axelrod, Jayshree Zaveri, Mikkel A Algire, Monzia Moodie, Michael G Montague, J Craig Venter, Hamilton O Smith, Clyde A Hutchison.
Abstract
We previously reported assembly and cloning of the synthetic Mycoplasma genitalium JCVI-1.0 genome in the yeast Saccharomyces cerevisiae by recombination of six overlapping DNA fragments to produce a 592-kb circle. Here we extend this approach by demonstrating assembly of the synthetic genome from 25 overlapping fragments in a single step. The use of yeast recombination greatly simplifies the assembly of large DNA molecules from both synthetic and natural fragments.Entities:
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Year: 2008 PMID： 19073939 PMCID： PMC2600582 DOI： 10.1073/pnas.0811011106
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205