Literature DB >> 851430

A simple enzymic method for the synthesis of adenosine 5'-[alpha-32P]triphosphate on a preparative scale.

B R Martin, H P Voorheis.   

Abstract

A simple, rapid and inexpensive method is described for the enzymic synthesis of [alpha-32P]ATP from [32P]Pi on a preparative scale with an overall yield of 53%. The final product contained all of the detectable radioactivity (less than 99.9%) in the alpha position and has been shown to behave identically with commerically availabe [alpha-32P]ATP during the synthesis of 3':5'-cyclic AMP in the reaction catalysed by adenylate cyclase.

Entities:  

Mesh：

Substances：

Year:  1977        PMID： 851430      PMCID： PMC1164541          DOI： 10.1042/bj1610555

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  8 in total

1.  A spectrophotometric method for the determination of creatine phosphokinase and myokinase.

Authors:  I T OLIVER
Journal:  Biochem J       Date:  1955-09       Impact factor: 3.857

2.  Enzymatic phosphorylation of adenosine and 2,6-diaminopurine riboside.

Authors:  A KORNBERG; W E PRICER
Journal:  J Biol Chem       Date:  1951-12       Impact factor: 5.157

Review 3.  Role of adenine and guanine nucleotides in the activity and response of adenylate cyclase systems to hormones: evidence for multisite transition states.

Authors:  M Rodbell; M C Lin; Y Salomon; C Londos; J P Harwood; B R Martin; M Rendell; M Berman
Journal:  Adv Cyclic Nucleotide Res       Date:  1975

4.  Effects of glucagon on cyclic AMP and carbohydrate metabolism in livers from diabetic rats.

Authors:  S J Pilkis; J H Exton; R A Johnson; C R Park
Journal:  Biochim Biophys Acta       Date:  1974-03-20

5.  A highly sensitive adenylate cyclase assay.

Authors:  Y Salomon; C Londos; M Rodbell
Journal:  Anal Biochem       Date:  1974-04       Impact factor: 3.365

6.  Modified procedure for the synthesis of 32P-labelled ribonucleoside 5'-monophosphates of high specific activity.

Authors:  R H Symons
Journal:  Biochim Biophys Acta       Date:  1968-02-26

7.  A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.

Authors:  I M Glynn; J B Chappell
Journal:  Biochem J       Date:  1964-01       Impact factor: 3.857

8.  Some properties of adenosine kinase from Ehrlich ascites-tumour cells.

Authors:  A W Murray
Journal:  Biochem J       Date:  1968-01       Impact factor: 3.857
  8 in total
  17 in total

1.  Transient complexes. A new structural model for the activation of adenylate cyclase by hormone receptors (guanine nucleotides/irradiation inactivation).

Authors:  B R Martin; J M Stein; E L Kennedy; C A Doberska; J C Metcalfe
Journal:  Biochem J       Date:  1979-11-15       Impact factor: 3.857

2.  A simple, rapid preparation of alpha[32P]-labelled adenosine diphosphate.

Authors:  I L Cartwright; D W Hutchinson
Journal:  Nucleic Acids Res       Date:  1977-07       Impact factor: 16.971

3.  Activation of adenylate cyclase in human platelet membranes by guanosine 5'-[beta gamma-imido]triphosphate is inhibited by cyclic-AMP-dependent phosphorylation. Slow activation occurs in the absence of ATP.

Authors:  R W Farndale; S K Wong; B R Martin
Journal:  Biochem J       Date:  1987-03-15       Impact factor: 3.857

4.  Adenylate cyclase in bloodstream forms of Trypanosoma (Trypanozoon) brucei sp.

Authors:  B R Martin; H P Voorheis; E L Kennedy
Journal:  Biochem J       Date:  1978-10-01       Impact factor: 3.857

5.  The role of GTP in prostaglandin E1 stimulation of adenylate cyclase in platelet membranes.

Authors:  J M Stein; B R Martin
Journal:  Biochem J       Date:  1983-07-15       Impact factor: 3.857

6.  The effect of fluoride on the state of aggregation of adenylate cyclase in rat liver plasma membranes.

Authors:  B R Martin; J M Stein; E L Kennedy; C A Doberska
Journal:  Biochem J       Date:  1980-04-15       Impact factor: 3.857

7.  The effect of alpha-adrenergic agonists on the membrane potential of fat-cell mitochondria in situ.

Authors:  R J Davis; B R Martin
Journal:  Biochem J       Date:  1982-09-15       Impact factor: 3.857

8.  The role of a guanine nucleotide-binding protein in the activation of rat liver plasma-membrane adenylate cyclase by forskolin.

Authors:  S K Wong; B R Martin
Journal:  Biochem J       Date:  1983-12-15       Impact factor: 3.857

9.  Evidence for regulation of human platelet adenylate cyclase by phosphorylation. Inhibition by ATP and guanosine 5'-[beta-thio]diphosphate occur by distinct mechanisms.

Authors:  I A Wadman; R W Farndale; B R Martin
Journal:  Biochem J       Date:  1991-06-15       Impact factor: 3.857

10.  A simple enzymic method for the synthesis of [32P]phosphoenolpyruvate.

Authors:  F Parra
Journal:  Biochem J       Date:  1982-09-01       Impact factor: 3.857
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.