Literature DB >> 909782

A simple, rapid preparation of alpha[32P]-labelled adenosine diphosphate.

I L Cartwright, D W Hutchinson.   

Abstract

Hexokinase (EC 2.7.1.1) will convert commercially available alpha-[(32)P]-labelled ATP into alpha-[(32)P]-labelled ADP. A simple, rapid isolation procedure for the alpha-[(32)P]-labelled ADP is described and this synthetic method can be used for the preparation of other alpha-[(32)P]-labelled nucleoside diphosphates.

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Year:  1977        PMID： 909782      PMCID： PMC342583          DOI： 10.1093/nar/4.7.2507

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  4 in total

1.  Thermostable polynucleotide phosphorylases from Bacillus stearothermophilus and Thermus aquaticus.

Authors:  J N Wood; D W Hutchinson
Journal:  Nucleic Acids Res       Date:  1976-01       Impact factor: 16.971

2.  Practical methods for the routine chemical synthesis of 32P-labelled nucleoside di- and triphosphates.

Authors:  R H Symons
Journal:  Biochim Biophys Acta       Date:  1970

3.  A simple enzymic method for the synthesis of adenosine 5'-[alpha-32P]triphosphate on a preparative scale.

Authors:  B R Martin; H P Voorheis
Journal:  Biochem J       Date:  1977-03-01       Impact factor: 3.857

4.  Preparation of [alpha-32P]nucleoside and deoxynucleoside 5'-triphosphates from 32Pi and protected and unprotected nucleosides.

Authors:  R H Symons
Journal:  Biochim Biophys Acta       Date:  1969-10-22
  4 in total
  1 in total

1.  Azidopolynucleotides as photoaffinity reagents.

Authors:  I L Cartwright; D W Hutchinson
Journal:  Nucleic Acids Res       Date:  1980-04-11       Impact factor: 16.971
  1 in total

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