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Literature DB >> 8486078

Non-uniform distribution of methylatable CCGG sequences on human chromosomes as shown by in situ methylation.

C Sentís¹, P Ludeña, J Fernandez-Piqueras.

Abstract

We carried out in situ methylation of human chromosomes with the HpaII methylase using [3H]methyl-S-adenosyl-L-methionine as the methyl group carrier. Autoradiographs localising [3H]methyl groups show methylatable CCGG sequences in the R-bands as well as in the short arms of the acrocentric chromosomes that include ribosomal DNA. The strongest labelling was observed over a subset of R-bands, including T-bands. Since methylatable CCGG sequences are representative of the unmethylated fraction of DNA, we suggest that differences in the degree of DNA methylation could be involved in the structure and function of chromosomal bands.

Entities: Chemical Species

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Year: 1993 PMID： 8486078 DOI： 10.1007/bf00352400

Source DB: PubMed Journal: Chromosoma ISSN： 0009-5915 Impact factor: 4.316

20 in total

1. Analysis of methylation and distribution of CpG sequences on human active and inactive X chromosomes by in situ nick translation.

Authors: G Prantera; M Ferraro
Journal: Chromosoma Date: 1990-04 Impact factor: 4.316

2. In situ nick translation of human metaphase chromosomes with the restriction enzymes MspI and HpaII reveals an R-band pattern.

Authors: S Adolph; H Hameister
Journal: Cytogenet Cell Genet Date: 1990

3. Correlation of pachytene chromomeres and metaphase bands of human chromosomes, and distinctive properties of telomeric regions.

Authors: P F Ambros; A T Sumner
Journal: Cytogenet Cell Genet Date: 1987

4. In situ methylation of human chromosomes with methylase-AluI.

Authors: J Fernández-Piqueras; P Ludeña; C Sentís
Journal: Cytogenet Cell Genet Date: 1989

Review 5. The isochore organization of the human genome.

Authors: G Bernardi
Journal: Annu Rev Genet Date: 1989 Impact factor: 16.830

6. [New system of chromosome banding: the T bands (author's transl)].

Authors: B Dutrillaux
Journal: Chromosoma Date: 1973-04-27 Impact factor: 4.316

7. Simultaneous fluorescent staining of R bands and specific heterochromatic regions (DA-DAPI bands) in human chromosomes.

Authors: D Schweizer
Journal: Cytogenet Cell Genet Date: 1980

Non-uniform distribution of methylatable CCGG sequences on human chromosomes as shown by in situ methylation.

1. Analysis of methylation and distribution of CpG sequences on human active and inactive X chromosomes by in situ nick translation.

2. In situ nick translation of human metaphase chromosomes with the restriction enzymes MspI and HpaII reveals an R-band pattern.

3. Correlation of pachytene chromomeres and metaphase bands of human chromosomes, and distinctive properties of telomeric regions.

4. In situ methylation of human chromosomes with methylase-AluI.

Review 5. The isochore organization of the human genome.

6. [New system of chromosome banding: the T bands (author's transl)].

7. Simultaneous fluorescent staining of R bands and specific heterochromatic regions (DA-DAPI bands) in human chromosomes.

8. Unmethylated domains in vertebrate DNA.

Review 9. Evolution of chromosome bands: molecular ecology of noncoding DNA.

10. Hemimethylated duplex DNAs prepared from 5-azacytidine-treated cells.

1. New sites of methylcytosine-rich DNA detected on metaphase chromosomes.

2. In human chromosomes telomeric regions are enriched in CpGs relative to R-bands.