| Literature DB >> 2612222 |
J Fernández-Piqueras1, P Ludeña, C Sentís.
Abstract
A method for in situ DNA methylation with the prokaryotic methylase AluI has been developed for use on fixed human chromosomes. Incorporation of methyl groups into the chromosomal DNA has been shown by autoradiography using a labeled substrate. The methylation prevents the digestion of chromosomal DNA by AluI, allowing direct visualization of clusters of nonmethylated AGCT targets along the human complement.Entities:
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Year: 1989 PMID： 2612222 DOI： 10.1159/000132850
Source DB: PubMed Journal: Cytogenet Cell Genet ISSN： 0301-0171